Q9AAS7 (Q9AAS7_CAUCR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase HAMAP MF_01374
EC=3.1.2.6 HAMAP MF_01374

Alternative name(s):
Glyoxalase II HAMAP MF_01374

Gene names

Name:	gloB HAMAP MF_01374
Ordered Locus Names:	CC_0519

Organism

Caulobacter crescentus (Caulobacter vibrioides)

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374 SAAS SAAS017782
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374 SAAS SAAS017782
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374 SAAS SAAS017782
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374 SAAS SAAS017782
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP MF_01374 SAAS SAAS017782 Zinc HAMAP MF_01374 SAAS SAAS017782
Molecular function	Hydrolase HAMAP MF_01374 SAAS SAAS017782 EMBL AAK22506.1
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	56	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	58	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	60	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	61	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	113	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	132	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	132	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	170	1	Zinc 2 By similarity HAMAP MF_01374

Sequences

	Sequence		Length	Mass (Da)
	Q9AAS7 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 991305F59AF21E61	FASTA	249	26,499
	10 20 30 40 50 60 MALTVHQFRC LSDNYGFLVR DEASGKVATI DTPDAGAILA ELAKLGWSLD LILNTHWHPD 70 80 90 100 110 120 HAGGNETLKT ATGATIVGPA EVTRIAPLDR VVRDGEEVML GDTRLTVIDT GGHTLGHVSY 130 140 150 160 170 180 YDAEDAIAFV GDTLFALGCG RLFEGTAEQM WDSLGRLIAL PDETTVYCAH EYTASNARFA 190 200 210 220 230 240 LSVDSSAALA ARAEAVFAAR ARDEATVPTT IGVEKATNPF LRAPLLRPDA PSPAQAFAEI RAAKDSFKG

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK22506.1.
PIR	F87313.
RefSeq	NP_419338.1. NC_002696.2.
3D structure databases
HSSP	HSSP built from PDB template 1QH5 based on UniProtKB Q16775.
ProteinModelPortal	Q9AAS7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	941843.
GenomeReviews	Gene locus CC_0519 in contig AE005673_GR.
KEGG	ccr:CC_0519.
NMPDR	fig|190650.1.peg.518.
PATRIC	21297984. VBICauCre124313_0529.
TIGR	CC_0519.
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
ProtClustDB	CLSK889979.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q9AAS7_CAUCR

Accession

Primary (citable) accession number: Q9AAS7

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)