Reviewed, UniProtKB/Swiss-Prot Q9AAK0 (CHEB2_CAUCR)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	CC_0597

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon HAMAP MF_00099		PRO_0000157985
Regions
	Domain	1 – 119	119	Response regulatory
	Domain	152 – 346	195	CheB-type methylesterase
Sites
	Active site	164	1	By similarity
	Active site	191	1	By similarity
	Active site	288	1	By similarity
Amino acid modifications
	Modified residue	52	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9AAK0-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 339DD0FA92B824DC	FASTA	347	36,098
	10 20 30 40 50 60 MRVMIVDDSA VVRGMVTRWL EEAGFIIAAI AVDGGQALRK LADTSVDVCL LDIEMPVMSG 70 80 90 100 110 120 LEALPKLLAA KPDLRVIMAS TLTERGAEVT LRALDLGAAD YIAKPSANKL GGADLYRKDL 130 140 150 160 170 180 IEKVRHLGAR AARPAPPQAA PRPTLAPPSS DPAGPIEAVV VASSTGGPPA LRRFIAGLGA 190 200 210 220 230 240 DWTSPILIAQ HMPPGFTRTL AQMLDPISHL TAREAQNGEP IVPGVIYVAP GDYHMTVRAA 250 260 270 280 290 300 GGGGPSIHLD QGPEVNYCRP SADPLFESAA RFWKGRVLGV VLTGMGRDGR DGAAVLAKVG 310 320 330 340 GTIIAQDEAT SVVWGMPGAV ANAGLAEAVL PLDQIGPYIA SRARSVA

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK22583.1.
PIR	C87323.
RefSeq	NP_419415.2.
3D structure databases
SMR	Q9AAK0. Positions 1-342.
ModBase	Search...
Genome annotation databases
GeneID	941679.
GenomeReviews	Gene locus CC_0597 in contig AE005673_GR.
KEGG	ccr:CC_0597.
NMPDR	fig|190650.1.peg.595.
TIGR	CC_0597.
Phylogenomic databases
HOGENOM	HBG705324.
OMA	QVVMMST.
Enzyme and pathway databases
BRENDA	3.1.1.61. 2191.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_CAUCR

Accession

Primary (citable) accession number: Q9AAK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2001
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)