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Q9AAA5

- SYI_CAUCR

UniProt

Q9AAA5 - SYI_CAUCR

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Protein

Isoleucine--tRNA ligase

Gene
ileS, CC_0701
Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei601 – 6011Aminoacyl-adenylate By similarity
Binding sitei645 – 6451ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCAULO:CC0701-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CC_0701
OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001816: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969969Isoleucine--tRNA ligaseUniRule annotationPRO_0000098374Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9AAA5.
SMRiQ9AAA5. Positions 58-878.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 8011"HIGH" regionUniRule annotationAdd
BLAST
Motifi642 – 6465"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9AAA5-1 [UniParc]FASTAAdd to Basket

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MADDATTARD YRETVFLPDT PFPMRAGLPK KEPEILEGWA ALSEKGLYGA    50
VRQKRQAAGA PLFVFHDGPP YANGAIHIGH ALNKILKDFV VRSRFALGYD 100
VDYVPGWDCH GLPIEWKIEE QFRAKGRRKD EVPAEEFRRE CRAYAGGWIE 150
AQKTEFQRLG VLGDWWNRYA TMDFTSEAKI VEEFHKFLAT GQLYRGSKPV 200
MWSPVERTAL ADAEIEYHDH VSPTIWVKFP VVQGSDAAVG AKLVIWTTTP 250
WTIPANRAVS YNPDIPYSVF EVTALEEGLE FEPWAKPGDR LIIADKLAED 300
VFKAAKVASW KTVEAVDCEG MVLAHPLADL DSHYGYAVPM LAGDHVTDDA 350
GTGFVHTAPG HGADDYQVWL AHGHREIPDT VDPDGAYYPH VALFAGLKVL 400
ETEGKKVGKF GPANGAVMEK LIEAGNLLAR GRVEHSYPHS WRSKAPVIFR 450
NTPQWFIRMD HAVDSLDGKT LREVAVQAIA DTAFYPDGGR NRIGAMVETR 500
PDWLISRQRN WGTPLAMFVD KHTGHPLNDP EVNARILAAI REGGADAWFT 550
RPDADFLGAH DPAQYEKITD ILDVWFDSGC THAFTIEGRA DSAWPADLYL 600
EGSDQHRGWF QSSLLEGCGT RGRAPYKAVV THGFTMDENG EKMSKSRGNT 650
IEPQTITKES GAEILRLWTA MVDYQEDQRI GKTILATTTD AYRKLRNTMR 700
YLLGALAGFD EEERVTDYDQ FPALEKYILH RLWELDGQVR EAYQSYRFSD 750
VIRPLIEFCQ GDLSALYFDV RRDSLYCDRP DALKRRAYRT ALDYVFDRLT 800
IWLAPLASFT MEEAWTTRFP EAGPVAYRVM PERVDAWRND AEAARWAKVE 850
KVTSVVTGAL EVERREKRIG SALEAAPVVH FADEDLLAAF EGLDAGEVFR 900
TSSATLVAGD AGAFRVDEVK GVSVDPNKAE GKKCARSWRI LPEVGTDPRY 950
PELSLRDADA VAYWDAGRG 969
Length:969
Mass (Da):108,637
Last modified:June 1, 2001 - v1
Checksum:iC7CEE8311F59B174
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005673 Genomic DNA. Translation: AAK22686.1.
PIRiB87336.
RefSeqiNP_419518.1. NC_002696.2.

Genome annotation databases

EnsemblBacteriaiAAK22686; AAK22686; CC_0701.
GeneIDi941463.
KEGGiccr:CC_0701.
PATRICi21298350. VBICauCre124313_0710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005673 Genomic DNA. Translation: AAK22686.1 .
PIRi B87336.
RefSeqi NP_419518.1. NC_002696.2.

3D structure databases

ProteinModelPortali Q9AAA5.
SMRi Q9AAA5. Positions 58-878.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK22686 ; AAK22686 ; CC_0701 .
GeneIDi 941463.
KEGGi ccr:CC_0701.
PATRICi 21298350. VBICauCre124313_0710.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CAULO:CC0701-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19089 / CB15.

Entry informationi

Entry nameiSYI_CAUCR
AccessioniPrimary (citable) accession number: Q9AAA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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