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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei601Aminoacyl-adenylateUniRule annotation1
Binding sitei645ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCAULO:CC0701-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CC_0701
OrganismiCaulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983741 – 969Isoleucine--tRNA ligaseAdd BLAST969

Proteomic databases

PRIDEiQ9AAA5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi190650.CC_0701

Structurei

3D structure databases

ProteinModelPortaliQ9AAA5
SMRiQ9AAA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi70 – 80"HIGH" regionAdd BLAST11
Motifi642 – 646"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiPSWYIRT

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9AAA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDATTARD YRETVFLPDT PFPMRAGLPK KEPEILEGWA ALSEKGLYGA
60 70 80 90 100
VRQKRQAAGA PLFVFHDGPP YANGAIHIGH ALNKILKDFV VRSRFALGYD
110 120 130 140 150
VDYVPGWDCH GLPIEWKIEE QFRAKGRRKD EVPAEEFRRE CRAYAGGWIE
160 170 180 190 200
AQKTEFQRLG VLGDWWNRYA TMDFTSEAKI VEEFHKFLAT GQLYRGSKPV
210 220 230 240 250
MWSPVERTAL ADAEIEYHDH VSPTIWVKFP VVQGSDAAVG AKLVIWTTTP
260 270 280 290 300
WTIPANRAVS YNPDIPYSVF EVTALEEGLE FEPWAKPGDR LIIADKLAED
310 320 330 340 350
VFKAAKVASW KTVEAVDCEG MVLAHPLADL DSHYGYAVPM LAGDHVTDDA
360 370 380 390 400
GTGFVHTAPG HGADDYQVWL AHGHREIPDT VDPDGAYYPH VALFAGLKVL
410 420 430 440 450
ETEGKKVGKF GPANGAVMEK LIEAGNLLAR GRVEHSYPHS WRSKAPVIFR
460 470 480 490 500
NTPQWFIRMD HAVDSLDGKT LREVAVQAIA DTAFYPDGGR NRIGAMVETR
510 520 530 540 550
PDWLISRQRN WGTPLAMFVD KHTGHPLNDP EVNARILAAI REGGADAWFT
560 570 580 590 600
RPDADFLGAH DPAQYEKITD ILDVWFDSGC THAFTIEGRA DSAWPADLYL
610 620 630 640 650
EGSDQHRGWF QSSLLEGCGT RGRAPYKAVV THGFTMDENG EKMSKSRGNT
660 670 680 690 700
IEPQTITKES GAEILRLWTA MVDYQEDQRI GKTILATTTD AYRKLRNTMR
710 720 730 740 750
YLLGALAGFD EEERVTDYDQ FPALEKYILH RLWELDGQVR EAYQSYRFSD
760 770 780 790 800
VIRPLIEFCQ GDLSALYFDV RRDSLYCDRP DALKRRAYRT ALDYVFDRLT
810 820 830 840 850
IWLAPLASFT MEEAWTTRFP EAGPVAYRVM PERVDAWRND AEAARWAKVE
860 870 880 890 900
KVTSVVTGAL EVERREKRIG SALEAAPVVH FADEDLLAAF EGLDAGEVFR
910 920 930 940 950
TSSATLVAGD AGAFRVDEVK GVSVDPNKAE GKKCARSWRI LPEVGTDPRY
960
PELSLRDADA VAYWDAGRG
Length:969
Mass (Da):108,637
Last modified:June 1, 2001 - v1
Checksum:iC7CEE8311F59B174
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA Translation: AAK22686.1
PIRiB87336
RefSeqiNP_419518.1, NC_002696.2
WP_010918587.1, NC_002696.2

Genome annotation databases

EnsemblBacteriaiAAK22686; AAK22686; CC_0701
GeneIDi941463
KEGGiccr:CC_0701
PATRICifig|190650.5.peg.710

Similar proteinsi

Entry informationi

Entry nameiSYI_CAUVC
AccessioniPrimary (citable) accession number: Q9AAA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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