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Q9AAA5

- SYI_CAUCR

UniProt

Q9AAA5 - SYI_CAUCR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei601 – 6011Aminoacyl-adenylateUniRule annotation
    Binding sitei645 – 6451ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCAULO:CC0701-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CC_0701
    OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
    Taxonomic identifieri190650 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
    ProteomesiUP000001816: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 969969Isoleucine--tRNA ligasePRO_0000098374Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ9AAA5.
    SMRiQ9AAA5. Positions 58-878.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi70 – 8011"HIGH" regionAdd
    BLAST
    Motifi642 – 6465"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9AAA5-1 [UniParc]FASTAAdd to Basket

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    MADDATTARD YRETVFLPDT PFPMRAGLPK KEPEILEGWA ALSEKGLYGA    50
    VRQKRQAAGA PLFVFHDGPP YANGAIHIGH ALNKILKDFV VRSRFALGYD 100
    VDYVPGWDCH GLPIEWKIEE QFRAKGRRKD EVPAEEFRRE CRAYAGGWIE 150
    AQKTEFQRLG VLGDWWNRYA TMDFTSEAKI VEEFHKFLAT GQLYRGSKPV 200
    MWSPVERTAL ADAEIEYHDH VSPTIWVKFP VVQGSDAAVG AKLVIWTTTP 250
    WTIPANRAVS YNPDIPYSVF EVTALEEGLE FEPWAKPGDR LIIADKLAED 300
    VFKAAKVASW KTVEAVDCEG MVLAHPLADL DSHYGYAVPM LAGDHVTDDA 350
    GTGFVHTAPG HGADDYQVWL AHGHREIPDT VDPDGAYYPH VALFAGLKVL 400
    ETEGKKVGKF GPANGAVMEK LIEAGNLLAR GRVEHSYPHS WRSKAPVIFR 450
    NTPQWFIRMD HAVDSLDGKT LREVAVQAIA DTAFYPDGGR NRIGAMVETR 500
    PDWLISRQRN WGTPLAMFVD KHTGHPLNDP EVNARILAAI REGGADAWFT 550
    RPDADFLGAH DPAQYEKITD ILDVWFDSGC THAFTIEGRA DSAWPADLYL 600
    EGSDQHRGWF QSSLLEGCGT RGRAPYKAVV THGFTMDENG EKMSKSRGNT 650
    IEPQTITKES GAEILRLWTA MVDYQEDQRI GKTILATTTD AYRKLRNTMR 700
    YLLGALAGFD EEERVTDYDQ FPALEKYILH RLWELDGQVR EAYQSYRFSD 750
    VIRPLIEFCQ GDLSALYFDV RRDSLYCDRP DALKRRAYRT ALDYVFDRLT 800
    IWLAPLASFT MEEAWTTRFP EAGPVAYRVM PERVDAWRND AEAARWAKVE 850
    KVTSVVTGAL EVERREKRIG SALEAAPVVH FADEDLLAAF EGLDAGEVFR 900
    TSSATLVAGD AGAFRVDEVK GVSVDPNKAE GKKCARSWRI LPEVGTDPRY 950
    PELSLRDADA VAYWDAGRG 969
    Length:969
    Mass (Da):108,637
    Last modified:June 1, 2001 - v1
    Checksum:iC7CEE8311F59B174
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005673 Genomic DNA. Translation: AAK22686.1.
    PIRiB87336.
    RefSeqiNP_419518.1. NC_002696.2.

    Genome annotation databases

    EnsemblBacteriaiAAK22686; AAK22686; CC_0701.
    GeneIDi941463.
    KEGGiccr:CC_0701.
    PATRICi21298350. VBICauCre124313_0710.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005673 Genomic DNA. Translation: AAK22686.1 .
    PIRi B87336.
    RefSeqi NP_419518.1. NC_002696.2.

    3D structure databases

    ProteinModelPortali Q9AAA5.
    SMRi Q9AAA5. Positions 58-878.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK22686 ; AAK22686 ; CC_0701 .
    GeneIDi 941463.
    KEGGi ccr:CC_0701.
    PATRICi 21298350. VBICauCre124313_0710.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CAULO:CC0701-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19089 / CB15.

    Entry informationi

    Entry nameiSYI_CAUCR
    AccessioniPrimary (citable) accession number: Q9AAA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3