Phenylalanine--tRNA ligase beta subunit - Caulobacter crescentus (strain ATCC 19089 / CB15)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	CC_1043

Organism

Caulobacter crescentus (strain ATCC 19089 / CB15) [Reference proteome] [HAMAP]

Taxonomic identifier

190650 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter ›

Protein attributes

Sequence length	799 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 799

799

Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283

PRO_0000126863

Regions

Domain

39 – 148

110

tRNA-binding

Domain

403 – 477

75

B5

Domain

706 – 798

93

FDX-ACB

Sites

Metal binding

455

1

Magnesium By similarity

Metal binding

461

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

464

1

Magnesium By similarity

Metal binding

465

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9A9E5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 70EA95DEB73B4180
Show »

FASTA

799

83,872

        10         20         30         40         50         60 
MKFTLSWLKD HLETTATVPE IVAAMTMAGL EVEHVIDPAT KLAPFTVCKI VEAARHPNAD 

        70         80         90        100        110        120 
RLQVCQVDTV DGRKEIVCGA PNARPGLTTI YAPIGAYVPG LDVTLVEKPV RGVVSNGMLC 

       130        140        150        160        170        180 
SAAELEYASE SDGIMELEGS LAVGTPAVDA LGLEAVIDFE VTPNRPDWLG VAGIARDLAA 

       190        200        210        220        230        240 
AGVGTLKDLS IAPIAGTYPS PISVTVDGEA CPAFAGRYIR GVKNGPSPKW LQDRLTAIGL 

       250        260        270        280        290        300 
RPINALVDVT NLISYDRARP LHVYDAHKLS GTQISTRLGR HGEHGDEHLI ALDGKTYELT 

       310        320        330        340        350        360 
PEMCVIADAN GERPIGLGGV MGGESTGCSE DTVDVFVESA WFEPIRIAQT GRTTGIASDA 

       370        380        390        400        410        420 
QYRFARTVDT GSLVPGIELA TKLILELCGG EPSEVVYVGE VPAAPKGFAF DPDYVGQLSG 

       430        440        450        460        470        480 
LAVTPEKTHQ ILAALGFDVV LGSPWTVTPP TFRRDVEGKA DLVEEVARIA GYGALPSTPL 

       490        500        510        520        530        540 
PEVPRAVGGI LTAKQARARA ARRALAAAGY AEAVTFSFTN RKTASLFGGG QDVLVLANPI 

       550        560        570        580        590        600 
ASELDCMRPS LLPNLIEAAG RNARQGFADA ALFEIGPTFH GDKPADQRTV VSAILAPKAP 

       610        620        630        640        650        660 
RGWDKAAQGD VFTVKADLLA LLEELGAPVA SLQTAQGSAS SWWHPGRSAR LQLGPKAVMA 

       670        680        690        700        710        720 
EFGEIHPAVL KALDVAGPVF GFEITLEAIP EPKKKSIKTK PAFSPSALMP LTRDFAFLVE 

       730        740        750        760        770        780 
KAKAAGDLVK AAAGADKALI TAARVFDVYE GPGVPDGFKS VAIEVVVQPR EATLTDAEIE 

       790 
ALSAKVVAAA EKAGGKLRS

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References

[1]

"Complete genome sequence of Caulobacter crescentus."
Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J.

Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK23027.1.
PIR	G87378.
RefSeq	NP_419859.1. NC_002696.2.
3D structure databases
ProteinModelPortal	Q9A9E5.
SMR	Q9A9E5. Positions 1-798.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK23027; AAK23027; CC_1043.
GeneID	940568.
KEGG	ccr:CC_1043.
PATRIC	21299058. VBICauCre124313_1059.
Phylogenomic databases
eggNOG	COG0073.
HOGENOM	HOG000292085.
KO	K01890.
OMA	ELERKCK.
ProtClustDB	PRK00629.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.56.20. 1 hit. 3.30.70.380. 1 hit. 3.50.40.10. 1 hit.
HAMAP	MF_00283. Phe_tRNA_synth_beta1.
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR004532. Phe-tRNA-ligase_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. pheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_CAUCR

Accession

Primary (citable) accession number: Q9A9E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents