Reviewed, UniProtKB/Swiss-Prot Q9A8J4 (RISB2_CAUCR)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase 2
      Short name=DMRL synthase 2
      Short name=Lumazine synthase 2
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase 2 beta chain

Gene names

Name:	ribH2
Ordered Locus Names:	CC_1359

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	6,7-dimethyl-8-ribityllumazine synthase 2 HAMAP MF_00178		PRO_0000134736

Sequences

	Sequence		Length	Mass (Da)
	Q9A8J4-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7EB2B6B48E7D83F1	FASTA	154	16,393
	10 20 30 40 50 60 MVEDKIRLLI VEGRSHSGIS DELLRGATQA IEARGADYDV ITVSNALQIP TVIAMAEDAG 70 80 90 100 110 120 HRPVGVRYDG YVALGVVIRG ETYHFELVAN ETARGLQDLG VAKRLPIGFG VLAVDEEAQA 130 140 150 LSRARVSEGD RGGAAAKACL ETIGLKRQLL GQAR

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK23340.1.
PIR	H87417.
RefSeq	NP_420172.1.
3D structure databases
HSSP	HSSP built from PDB template 1C41 based on UniProtKB Q9UVT8.
ModBase	Search...
Genome annotation databases
GeneID	941698.
GenomeReviews	Gene locus CC_1359 in contig AE005673_GR.
KEGG	ccr:CC_1359.
NMPDR	fig|190650.1.peg.1352.
TIGR	CC_1359.
Phylogenomic databases
HOGENOM	Q9A8J4.
OMA	TIHFEIV.
Enzyme and pathway databases
BRENDA	2.5.1.9. 2191.
Family and domain databases
HAMAP	MF_00178. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
ProDom	PD003664. DMRL_synthase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00114. lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB2_CAUCR

Accession

Primary (citable) accession number: Q9A8J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)