Reviewed, UniProtKB/Swiss-Prot Q9A8J0 (HPPA_CAUCR)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	CC_1363

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	712 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 712	712	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217013
Regions
	Transmembrane	2 – 24	23	Potential
	Transmembrane	54 – 73	20	Potential
	Transmembrane	80 – 102	23	Potential
	Transmembrane	128 – 150	23	Potential
	Transmembrane	163 – 185	23	Potential
	Transmembrane	232 – 251	20	Potential
	Transmembrane	258 – 280	23	Potential
	Transmembrane	290 – 312	23	Potential
	Transmembrane	333 – 355	23	Potential
	Transmembrane	392 – 414	23	Potential
	Transmembrane	472 – 489	18	Potential
	Transmembrane	515 – 537	23	Potential
	Transmembrane	588 – 610	23	Potential
	Transmembrane	614 – 636	23	Potential
Sites
	Site	466	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9A8J0-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9092102AF55990C4	FASTA	712	72,944
	10 20 30 40 50 60 MSLWLYLAIG AGLLAVLYGA VQTASLMRAS AGNARMQEIA AAIQEGAQAY LKRQYTTISI 70 80 90 100 110 120 VGVVVIAALA FFFKSWEQPV GFALGAILSG AAGFAGMLIS VRANVRTAQA SSESLAKGLS 130 140 150 160 170 180 MAFTSGAVTG MLVAGFALLG VAGYYYVLLA TGHEATGRVV IDSLVALGFG ASLISIFARL 190 200 210 220 230 240 GGGIFTKGAD VGGDLVGKVE AGIPEDDPRN AATIADNVGD NVGDCAGMAA DLFETYAVTT 250 260 270 280 290 300 VATMVLAAIF FRGTEAVSAM MLLPLAICAV CIVTSIIGAF FVRLGKSQNI MGALYQGLIV 310 320 330 340 350 360 TGVLSIPAVW YVIHQLVPTA VEVDGRSYGA DALFYCGLAG LVVTAAIVMI TEYYTGTGFR 370 380 390 400 410 420 PVKSVAQASV SGHGTNVIQG LAMSLESTAL PALTIIVGIV VTYNLAGLFG IAIATTTMLS 430 440 450 460 470 480 LAGFIVALDA FGPVTDNAGG IAEMAGLPPE VRVTTDALDA VGNTTKAVTK GYAIGSAGLG 490 500 510 520 530 540 ALVLFAAYTE DLKFFSENAA PGSFFHGMGA VTFDLSNPYV VVGLLFGGLL PFLFGGLSMT 550 560 570 580 590 600 AVGRAAESVV AEVRRQFRDN PGIMTGEVKP EYGKAVDILT KAAIREMIVP SLLPVVSPVA 610 620 630 640 650 660 LFFVIQAIAG KVDAFAALGA MLMGVIVTGL FVAISMTSGG GAWDNAKKVI EEGFTDKNGV 670 680 690 700 710 LHKKGGETHK AAVTGDTVGD PYKDTSGPAV NPMIKITNIV ALLLLAVLAH GV

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK23344.1.
PIR	D87418.
RefSeq	NP_420176.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	941699.
GenomeReviews	Gene locus CC_1363 in contig AE005673_GR.
KEGG	ccr:CC_1363.
NMPDR	fig|190650.1.peg.1356.
TIGR	CC_1363.
Phylogenomic databases
HOGENOM	Q9A8J0.
OMA	PYLFGGI.
Enzyme and pathway databases
BRENDA	3.6.1.1. 2191.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_CAUCR

Accession

Primary (citable) accession number: Q9A8J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)