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Protein

K(+)-insensitive pyrophosphate-energized proton pump

Gene

hppA

Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei179 – 1791Important for ion transportBy similarity
Binding sitei187 – 1871SubstrateBy similarity
Metal bindingi190 – 1901Magnesium 1By similarity
Metal bindingi194 – 1941Magnesium 1By similarity
Metal bindingi217 – 2171Magnesium 2By similarity
Metal bindingi220 – 2201Magnesium 2By similarity
Sitei224 – 2241Important for ion transportBy similarity
Sitei231 – 2311Important for ion transportBy similarity
Metal bindingi436 – 4361Magnesium 2By similarity
Sitei466 – 4661Important for potassium independenceBy similarity
Metal bindingi644 – 6441Calcium or magnesiumBy similarity
Metal bindingi676 – 6761Calcium or magnesiumBy similarity
Metal bindingi680 – 6801Calcium or magnesiumBy similarity
Binding sitei683 – 6831SubstrateBy similarity
Sitei684 – 6841Important for ion transportBy similarity
Sitei695 – 6951Important for ion transportBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCAULO:CC1363-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-insensitive pyrophosphate-energized proton pumpUniRule annotation (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound proton-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
H(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:CC_1363
OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001816 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalUniRule annotationAdd
BLAST
Transmembranei56 – 7621HelicalUniRule annotationAdd
BLAST
Transmembranei79 – 9921HelicalUniRule annotationAdd
BLAST
Transmembranei130 – 15021HelicalUniRule annotationAdd
BLAST
Transmembranei165 – 18521HelicalUniRule annotationAdd
BLAST
Transmembranei231 – 25121HelicalUniRule annotationAdd
BLAST
Transmembranei262 – 28221HelicalUniRule annotationAdd
BLAST
Transmembranei293 – 31321HelicalUniRule annotationAdd
BLAST
Transmembranei330 – 35021HelicalUniRule annotationAdd
BLAST
Transmembranei393 – 41321HelicalUniRule annotationAdd
BLAST
Transmembranei414 – 43421HelicalUniRule annotationAdd
BLAST
Transmembranei467 – 48721HelicalUniRule annotationAdd
BLAST
Transmembranei519 – 53921HelicalUniRule annotationAdd
BLAST
Transmembranei588 – 60821HelicalUniRule annotationAdd
BLAST
Transmembranei614 – 63421HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 712712K(+)-insensitive pyrophosphate-energized proton pumpPRO_0000217013Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi190650.CC_1363.

Structurei

3D structure databases

ProteinModelPortaliQ9A8J0.
SMRiQ9A8J0. Positions 38-711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiGWKGILF.
OrthoDBiEOG6TXQSF.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9A8J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLWLYLAIG AGLLAVLYGA VQTASLMRAS AGNARMQEIA AAIQEGAQAY
60 70 80 90 100
LKRQYTTISI VGVVVIAALA FFFKSWEQPV GFALGAILSG AAGFAGMLIS
110 120 130 140 150
VRANVRTAQA SSESLAKGLS MAFTSGAVTG MLVAGFALLG VAGYYYVLLA
160 170 180 190 200
TGHEATGRVV IDSLVALGFG ASLISIFARL GGGIFTKGAD VGGDLVGKVE
210 220 230 240 250
AGIPEDDPRN AATIADNVGD NVGDCAGMAA DLFETYAVTT VATMVLAAIF
260 270 280 290 300
FRGTEAVSAM MLLPLAICAV CIVTSIIGAF FVRLGKSQNI MGALYQGLIV
310 320 330 340 350
TGVLSIPAVW YVIHQLVPTA VEVDGRSYGA DALFYCGLAG LVVTAAIVMI
360 370 380 390 400
TEYYTGTGFR PVKSVAQASV SGHGTNVIQG LAMSLESTAL PALTIIVGIV
410 420 430 440 450
VTYNLAGLFG IAIATTTMLS LAGFIVALDA FGPVTDNAGG IAEMAGLPPE
460 470 480 490 500
VRVTTDALDA VGNTTKAVTK GYAIGSAGLG ALVLFAAYTE DLKFFSENAA
510 520 530 540 550
PGSFFHGMGA VTFDLSNPYV VVGLLFGGLL PFLFGGLSMT AVGRAAESVV
560 570 580 590 600
AEVRRQFRDN PGIMTGEVKP EYGKAVDILT KAAIREMIVP SLLPVVSPVA
610 620 630 640 650
LFFVIQAIAG KVDAFAALGA MLMGVIVTGL FVAISMTSGG GAWDNAKKVI
660 670 680 690 700
EEGFTDKNGV LHKKGGETHK AAVTGDTVGD PYKDTSGPAV NPMIKITNIV
710
ALLLLAVLAH GV
Length:712
Mass (Da):72,944
Last modified:June 1, 2001 - v1
Checksum:i9092102AF55990C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK23344.1.
PIRiD87418.
RefSeqiNP_420176.1. NC_002696.2.
WP_010919240.1. NC_002696.2.

Genome annotation databases

EnsemblBacteriaiAAK23344; AAK23344; CC_1363.
GeneIDi941699.
KEGGiccr:CC_1363.
PATRICi21299742. VBICauCre124313_1393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK23344.1.
PIRiD87418.
RefSeqiNP_420176.1. NC_002696.2.
WP_010919240.1. NC_002696.2.

3D structure databases

ProteinModelPortaliQ9A8J0.
SMRiQ9A8J0. Positions 38-711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190650.CC_1363.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK23344; AAK23344; CC_1363.
GeneIDi941699.
KEGGiccr:CC_1363.
PATRICi21299742. VBICauCre124313_1393.

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiGWKGILF.
OrthoDBiEOG6TXQSF.

Enzyme and pathway databases

BioCyciCAULO:CC1363-MONOMER.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19089 / CB15.

Entry informationi

Entry nameiHPPA_CAUCR
AccessioniPrimary (citable) accession number: Q9A8J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2001
Last modified: November 11, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.