Reviewed, UniProtKB/Swiss-Prot Q9A7M9 (AMPA_CAUCR)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	CC_1692

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165736
Sites
	Active site	270	1	Potential
	Active site	344	1	Potential
	Metal binding	258	1	Manganese 2 By similarity
	Metal binding	263	1	Manganese 1 By similarity
	Metal binding	263	1	Manganese 2 By similarity
	Metal binding	281	1	Manganese 2 By similarity
	Metal binding	340	1	Manganese 1 By similarity
	Metal binding	342	1	Manganese 1 By similarity
	Metal binding	342	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9A7M9-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EFD8D1075C2334A5	FASTA	493	51,175
	10 20 30 40 50 60 MRIEFVPVDT QAGATAALAV LAHEGAALSP EARAANEATG GALARAIAGG RFTGAKGQTL 70 80 90 100 110 120 DIVAPNGLEA ARVVLVGVDG SGAVEPEAVE LAAASAFQAV KTSGVVELVL KLGADAETAA 130 140 150 160 170 180 RAAFGARLAA YRFDKYRTTE KAEKKPSVQV VKIAAADPVK AQKAYEPLAA LADAIVFSRN 190 200 210 220 230 240 LVSEPANILH PEEFAARAKG LESLGLEVEI LGEAEMAKLG MGSLLGVGQG SVRESQLVIM 250 260 270 280 290 300 KWMGAADKSA QPIAFVGKGV CFDTGGISIK PADGMEDMKW DMGGAAAVAG VMHALAGRKA 310 320 330 340 350 360 KVNAIGVLGL VENMPDGNAQ RPGDVVTSMS GQTIEVINTD AEGRLVLADA LWYTQERFKP 370 380 390 400 410 420 QFMIDLATLT GAIIISLGHD YAGLFSNNDG LSEKLLAAGK AERESLWRLP LPAAYEKQIE 430 440 450 460 470 480 SPIADMKNIG GRPAGSITAG LFLQKFVNGV PWAHLDIASV AWKKPSADPT VPDGAVGYGV 490 RLLNRLVADA YEG

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK23669.1.
PIR	A87459.
RefSeq	NP_420501.1.
3D structure databases
HSSP	HSSP built from PDB template 1GYT based on UniProtKB P11648.
ModBase	Search...
Genome annotation databases
GeneID	942053.
GenomeReviews	Gene locus CC_1692 in contig AE005673_GR.
KEGG	ccr:CC_1692.
NMPDR	fig|190650.1.peg.1681.
TIGR	CC_1692.
Phylogenomic databases
HOGENOM	Q9A7M9.
OMA	ECEDLGM.
Enzyme and pathway databases
BRENDA	3.4.11.1. 2191.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_CAUCR

Accession

Primary (citable) accession number: Q9A7M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)