Q9A734 (SYD_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	CC_1892

Organism

Caulobacter crescentus (Caulobacter vibrioides)

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 609	609	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110851

Sequences

	Sequence		Length	Mass (Da)
	Q9A734 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A93D8380B5928042	FASTA	609	67,988
	10 20 30 40 50 60 MHAYRTHNCG ALRASDTGAA VRLSGWIHRK RDHGGLVFID LRDHYGLTQL VLHPETPGFN 70 80 90 100 110 120 VVERLRAESV IRVDGEVIAR DASVVNPNLP TGEIEIRVSA VEVLSEAAEL PLPVFGEPDY 130 140 150 160 170 180 PEEIRLKHRY LDLRRETLHK NIVLRSRVIQ SIRNRMFAQG FNEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRLHPEK FYALPQAPQQ FKQLLMVSGF DRYFQIAPCF RDEDLRADRS LEFYQLDVEM 250 260 270 280 290 300 SFVTQEDVFA AIEPVMHGVF EEFSNGKPVS PINGTHTFTN DFGQSFEHKG FERLTYAQSM 310 320 330 340 350 360 AWYGSDKPDL RNPIKMANVS EHFRDGGFGL FAKILGADAK NQVWAIPAPT GGSRAFCDRM 370 380 390 400 410 420 NSWAQGEGQP GLGYVFWSED QGGWGGPIAK NLGEPTQALM ESLGLGAGDA AFFVAGDPAV 430 440 450 460 470 480 FAKFAGLART RVGTELKLVD ENQFKFCWIV DFPMFEWNEE EKKVDFSHNP FSMPQGGLEA 490 500 510 520 530 540 LETQDPLTIR AYQYDIVCNG YELCSGAIRN HKPEIMLKAF ATAGYGPEVV EEQFGGMLNA 550 560 570 580 590 600 FRYGAPPHGG LAPGIDRIVM LLADQVAIRE VIAFPLNQQG QDLLMNAPAN VLDKQLKELH IRTAPPIKV

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK23867.1.
PIR	G87483.
RefSeq	NP_420699.1. NC_002696.2.
3D structure databases
ProteinModelPortal	Q9A734.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	942550.
GenomeReviews	Gene locus CC_1892 in contig AE005673_GR.
KEGG	ccr:CC_1892.
NMPDR	fig|190650.1.peg.1879.
PATRIC	21300786. VBICauCre124313_1908.
TIGR	CC_1892.
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_CAUCR

Accession

Primary (citable) accession number: Q9A734

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program