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Protein

Response regulator PleD

Gene

pleD

Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Response regulator that is part of a signal transduction pathway controlling cell differentiation in the swarmer-to-stalked cell transition.By similarity
Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger.By similarity

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.

Enzyme regulationi

Allosterically inhibited by the product c-di-GMP.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91MagnesiumBy similarity
Metal bindingi10 – 101MagnesiumBy similarity
Metal bindingi53 – 531MagnesiumBy similarity
Metal bindingi55 – 551MagnesiumBy similarity
Sitei178 – 1781Allosteric product bindingBy similarity
Sitei332 – 3321Transition state stabilizerSequence analysis
Binding sitei335 – 3351SubstrateBy similarity
Binding sitei344 – 3441SubstrateBy similarity
Sitei359 – 3591Allosteric product phosphate group bindingBy similarity
Sitei362 – 3621Allosteric product bindingBy similarity
Active sitei370 – 3701Proton acceptorSequence analysis
Sitei390 – 3901Allosteric product bindingBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer, Transferase

Keywords - Biological processi

Cell cycle, Differentiation, Two-component regulatory system

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCAULO:CC2462-MONOMER.
BRENDAi2.7.7.65. 1218.
UniPathwayiUPA00599.

Names & Taxonomyi

Protein namesi
Recommended name:
Response regulator PleD
Alternative name(s):
Stalked cell differentiation-controlling protein
Including the following 1 domains:
Diguanylate cyclase (EC:2.7.7.65)
Short name:
DGC
Alternative name(s):
Diguanylate kinase
Gene namesi
Name:pleD
Ordered Locus Names:CC_2462
OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Phosphorylated PleD localizes to the differentiating pole.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Response regulator PleDPRO_0000081206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 5314-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by PleC and DivJ. Phosphorylation stimulates cyclase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Inactive monomer in solution (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
divJQ032282EBI-1784732,EBI-1785038
pleCP378943EBI-1784732,EBI-1784742

Protein-protein interaction databases

DIPiDIP-29503N.
IntActiQ9A5I5. 2 interactions.
STRINGi190650.CC_2462.

Structurei

3D structure databases

ProteinModelPortaliQ9A5I5.
SMRiQ9A5I5. Positions 2-454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 120117Response regulatory 1PROSITE-ProRule annotationAdd
BLAST
Domaini155 – 269115Response regulatory 2PROSITE-ProRule annotationAdd
BLAST
Domaini319 – 454136GGDEFPROSITE-ProRule annotationAdd
BLAST

Domaini

Activated by phosphorylation at the first response regulatory domain, which induces dimerization mediated by the two response regulatory domains and allows the two substrate-binding sites to approach each other and the condensation reaction to occur (Probable). The diguanylate cyclase activity is harbored by the GGDEF domain (By similarity).By similarityCurated

Sequence similaritiesi

Contains 1 GGDEF domain.PROSITE-ProRule annotation
Contains 2 response regulatory domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000167204.
KOiK02488.
OMAiQESANRD.
OrthoDBiEOG6G4VQG.

Family and domain databases

InterProiIPR011006. CheY-like_superfamily.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00990. GGDEF. 1 hit.
PF00072. Response_reg. 2 hits.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
SM00448. REC. 2 hits.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 2 hits.
SSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
PS50110. RESPONSE_REGULATORY. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9A5I5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARILVVDD IEANVRLLEA KLTAEYYEVS TAMDGPTALA MAARDLPDII
60 70 80 90 100
LLDVMMPGMD GFTVCRKLKD DPTTRHIPVV LITALDGRGD RIQGLESGAS
110 120 130 140 150
DFLTKPIDDV MLFARVRSLT RFKLVIDELR QREASGRRMG VIAGAAARLD
160 170 180 190 200
GLGGRVLIVD DNERQAQRVA AELGVEHRPV IESDPEKAKI SAGGPVDLVI
210 220 230 240 250
VNAAAKNFDG LRFTAALRSE ERTRQLPVLA MVDPDDRGRM VKALEIGVND
260 270 280 290 300
ILSRPIDPQE LSARVKTQIQ RKRYTDYLRN NLDHSLELAV TDQLTGLHNR
310 320 330 340 350
RYMTGQLDSL VKRATLGGDP VSALLIDIDF FKKINDTFGH DIGDEVLREF
360 370 380 390 400
ALRLASNVRA IDLPCRYGGE EFVVIMPDTA LADALRIAER IRMHVSGSPF
410 420 430 440 450
TVAHGREMLN VTISIGVSAT AGEGDTPEAL LKRADEGVYQ AKASGRNAVV

GKAA
Length:454
Mass (Da):49,624
Last modified:June 1, 2001 - v1
Checksum:iD44909D42B581516
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 484DLPD → ICPT in AAA87378 (PubMed:7592388).Curated
Sequence conflicti274 – 2741Y → C in AAA87378 (PubMed:7592388).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42554 Genomic DNA. Translation: AAA87378.1.
AE005673 Genomic DNA. Translation: AAK24433.1.
PIRiE87554.
RefSeqiNP_421265.1. NC_002696.2.
WP_010920320.1. NC_002696.2.

Genome annotation databases

EnsemblBacteriaiAAK24433; AAK24433; CC_2462.
GeneIDi943650.
KEGGiccr:CC_2462.
PATRICi21301956. VBICauCre124313_2479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42554 Genomic DNA. Translation: AAA87378.1.
AE005673 Genomic DNA. Translation: AAK24433.1.
PIRiE87554.
RefSeqiNP_421265.1. NC_002696.2.
WP_010920320.1. NC_002696.2.

3D structure databases

ProteinModelPortaliQ9A5I5.
SMRiQ9A5I5. Positions 2-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29503N.
IntActiQ9A5I5. 2 interactions.
STRINGi190650.CC_2462.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK24433; AAK24433; CC_2462.
GeneIDi943650.
KEGGiccr:CC_2462.
PATRICi21301956. VBICauCre124313_2479.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000167204.
KOiK02488.
OMAiQESANRD.
OrthoDBiEOG6G4VQG.

Enzyme and pathway databases

UniPathwayiUPA00599.
BioCyciCAULO:CC2462-MONOMER.
BRENDAi2.7.7.65. 1218.

Family and domain databases

InterProiIPR011006. CheY-like_superfamily.
IPR000160. GGDEF_dom.
IPR029787. Nucleotide_cyclase.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF00990. GGDEF. 1 hit.
PF00072. Response_reg. 2 hits.
[Graphical view]
SMARTiSM00267. GGDEF. 1 hit.
SM00448. REC. 2 hits.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 2 hits.
SSF55073. SSF55073. 1 hit.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
PROSITEiPS50887. GGDEF. 1 hit.
PS50110. RESPONSE_REGULATORY. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel response regulator required for the swarmer-to-stalked-cell transition in Caulobacter crescentus."
    Hecht G.B., Newton A.
    J. Bacteriol. 177:6223-6229(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 19089 / CB15.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19089 / CB15.

Entry informationi

Entry nameiPLED_CAUCR
AccessioniPrimary (citable) accession number: Q9A5I5
Secondary accession number(s): Q46020
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2001
Last modified: November 11, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.