Q9A5F0 (PURL_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	CC_2500

Organism

Caulobacter crescentus (strain ATCC 19089 / CB15) [Reference proteome] [HAMAP]

Taxonomic identifier

190650 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter › Caulobacter crescentus

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 739	739	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_0000100448
Regions
	Nucleotide binding	109 – 120	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9A5F0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 942A0741D9E858F5	FASTA	739	77,704
	10 20 30 40 50 60 MSTSSTPSAK PMAEMAAEFG LKPAEYDVVL KRLGREPNLV ELGVFSVMWS EHCSYKSSKN 70 80 90 100 110 120 QLKKFPIDGP RVICGPGENA GVIDIGDGDA IIFKMESHNH PSYIEPYQGA ATGVGGIMRD 130 140 150 160 170 180 VFTMGARPIA LLNALRFGDP SHPKTKRLVD GVVAGIAGYG NCVGVPTVAG ETNFHKGYNG 190 200 210 220 230 240 NILVNAMCVG LAKADSIFYS AAPGPGLAVV YFGSKTGRDG IHGATMSSAE FSEDSEEKRP 250 260 270 280 290 300 TVQVGDPFAE KLLIEATLEL MATGAVAAIQ DMGAAGLTSS SVEMAGKGGV GIELNMDMVP 310 320 330 340 350 360 QRETGMSAYE MMLSESQERM LAVLKPGREQ DGHAIFEKWG LDAAVIGYTT DTGRLVLKHH 370 380 390 400 410 420 GETVCDVPLA PLFDDAPLYD RPWVQPALQP RLDPAAVPAP TNWNEAVLKI IGCPDMASKR 430 440 450 460 470 480 WLWEQYDRHV MADTLEDSAT GCDAGIVRIH GTGKAIAVTS DCTPRYVQAD PYEGGKQAVA 490 500 510 520 530 540 EAWRNLTAAG ALPIAITDNL NFGSPEKPET MGQIVRATDG MAEACRALDF PVVSGNVSLY 550 560 570 580 590 600 NETNGVAIPP TPTVGGVGLL EDYDLRTGFG NVAEGDTLVL VGETRGELGA SIYLREILGR 610 620 630 640 650 660 EDGAPPPVDL ALERKTGDFV RGLISSGLVA GVHDLSDGGL LVAAADVALA SKIGVTLNAT 670 680 690 700 710 720 SQTHAHAYLL GEDQARYLIA TPDPDAVLEA AKEAGVHANV AGVAGGEAFA SDGLFSVSLD 730 ALRAAHEAWL PGYMSAPKA

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK24471.1.
PIR	C87559.
RefSeq	NP_421303.1. NC_002696.2.
3D structure databases
ProteinModelPortal	Q9A5F0.
SMR	Q9A5F0. Positions 12-730.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK24471; AAK24471; CC_2500.
GeneID	944037.
KEGG	ccr:CC_2500.
PATRIC	21302034. VBICauCre124313_2518.
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_CAUCR

Accession

Primary (citable) accession number: Q9A5F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program