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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei283 – 2831Proton acceptorUniRule annotation
Metal bindingi326 – 3261IronUniRule annotation
Metal bindingi332 – 3321IronUniRule annotation
Binding sitei341 – 3411homogentisateUniRule annotation
Metal bindingi362 – 3621IronUniRule annotation
Binding sitei362 – 3621homogentisateUniRule annotation

GO - Molecular functioni

  1. homogentisate 1,2-dioxygenase activity Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-phenylalanine catabolic process Source: UniProtKB-HAMAP
  2. tyrosine catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciCAULO:CC2532-MONOMER.
UniPathwayiUPA00139; UER00339.

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:CC_2532
OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001816: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Homogentisate 1,2-dioxygenasePRO_0000220247Add
BLAST

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9A5B8.
SMRiQ9A5B8. Positions 2-420.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9A5B8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLRYQTGFG SYFETEAHPG ALPVGRNSPQ KVPFGLYAEQ LSGSAFTAPR
60 70 80 90 100
HENRRTWLYR LRPSAGHEAY QPYAQDKLVS AFPGPATPNR LRWSPLEIPA
110 120 130 140 150
APTDFVDGLV SLAGNADAAT LGGIAAHVYL VNVSMKNRVF YNADGEMLIV
160 170 180 190 200
PQMGELTLVT EMGVLKAGPG HIAVIPRGVR FRVEVEGPAR GYVCENYGAA
210 220 230 240 250
FRLPELGPIG ANGLANPRDF EAPVAAFEDI DTPTQVIQKF QGALWAATWD
260 270 280 290 300
HSPLDVVAWH GNLTPYRYDL SRFNTINTVS YDHPDPSIFT VLTSPSDTPG
310 320 330 340 350
TANCDFVIFP PRWMVAEDTF RPPWFHRNVM SEFMGLIHGA YDAKAGGFVP
360 370 380 390 400
GGASLHNCMS DHGPDVASHK KATEVVLAPH KIDGTMAFMF ESRWVFRPTH
410 420
LALESAALQS DYDACWTGFP KARLS
Length:425
Mass (Da):46,607
Last modified:June 1, 2001 - v1
Checksum:iB61AE29016AD9C27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK24503.1.
PIRiC87563.
RefSeqiNP_421335.1. NC_002696.2.

Genome annotation databases

EnsemblBacteriaiAAK24503; AAK24503; CC_2532.
GeneIDi943950.
KEGGiccr:CC_2532.
PATRICi21302090. VBICauCre124313_2546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK24503.1.
PIRiC87563.
RefSeqiNP_421335.1. NC_002696.2.

3D structure databases

ProteinModelPortaliQ9A5B8.
SMRiQ9A5B8. Positions 2-420.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK24503; AAK24503; CC_2532.
GeneIDi943950.
KEGGiccr:CC_2532.
PATRICi21302090. VBICauCre124313_2546.

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.
BioCyciCAULO:CC2532-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19089 / CB15.

Entry informationi

Entry nameiHGD_CAUCR
AccessioniPrimary (citable) accession number: Q9A5B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.