Reviewed, UniProtKB/Swiss-Prot Q9A5A7 (MURB_CAUCR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	CC_2545

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179193
Regions
	Domain	27 – 194	168	FAD-binding PCMH-type
Sites
	Active site	172	1	By similarity
	Active site	223	1	Proton donor By similarity
	Active site	293	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9A5A7-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 05D974DCB04C16E7	FASTA	301	31,918
	10 20 30 40 50 60 MTWKTQLPTA RGKLLIDEAL APFTWFRVGG PADVVFLPAD EQDLSDFLKG LDPSVPVMAI 70 80 90 100 110 120 GVGSNLLVRD GGVDGVVIRL GKGFNGVEAL GDNRIKAGSA VPDAILARKA AEAGIAGLEF 130 140 150 160 170 180 YVGVPGTIGG AVIMNAGCYG AETVNVVKSV RVMNRAGVVR ELSVEDLHYT YRHSALQDGE 190 200 210 220 230 240 PVIVLDAIFE GTPDEPEAIK ARMAEITARR ETTQPIREKT GGSTFKNPPG HSSWKLVDEA 250 260 270 280 290 300 GWRGKPYGGA MFSPLHSNFL INTGEATAAD LEGLGEAVRA DVLAKTGVQL DWEIKRIGRA G

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK24516.1.
PIR	H87564.
RefSeq	NP_421348.1.
3D structure databases
HSSP	HSSP built from PDB template 1HSK based on UniProtKB Q93G02.
ModBase	Search...
Genome annotation databases
GeneID	941332.
GenomeReviews	Gene locus CC_2545 in contig AE005673_GR.
KEGG	ccr:CC_2545.
NMPDR	fig|190650.1.peg.2528.
TIGR	CC_2545.
Phylogenomic databases
HOGENOM	Q9A5A7.
OMA	QPIREKT.
Enzyme and pathway databases
BRENDA	1.1.1.158. 2191.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_CAUCR

Accession

Primary (citable) accession number: Q9A5A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)