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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Caulobacter crescentus (strain ATCC 19089 / CB15)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 196FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciCAULO:CC2598-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:CC_2598
OrganismiCaulobacter crescentus (strain ATCC 19089 / CB15)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117241Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190650.CC_2598.

Structurei

3D structure databases

ProteinModelPortaliQ9A566.
SMRiQ9A566. Positions 10-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9A566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTNSTYQPV HVIGGGLAGS EAAWQIAQSG VPVILHEMRK DDATGKVITD
60 70 80 90 100
AHQTDGLAEM VCSNSFRSDD WQFNAVGLLH AEMRKLGSLI MSCADQHQVP
110 120 130 140 150
AGGALAVDRD GFSTEVTKRL SQHPLVTIVR EEIAGLPPAP DGKNGWDNVI
160 170 180 190 200
VATGPLTSPA LAQAVLDLTG EGQLSFFDAI APIIHFESIN MDIAWRQSRY
210 220 230 240 250
DKEGPGGDAA AYINCPMNKE QYEAFIDALL AGPKSEFKEW ENVPYFDGCL
260 270 280 290 300
PIEVMAERGR ETLRHGPMKP VGLTNPRDPL VKAYAIVQLR QDNALGTLWN
310 320 330 340 350
MVGFQTKLKH GVQAETFRMI PGLEDAQFAR LGGLHRNTFI NSPKLLDKSL
360 370 380 390 400
RMKAQPRLRF AGQVTGVEGY VESAAMGLLT GRFAAADRKG APIDAPPPTT
410 420 430 440 450
ALGALVEHIT GGHLEAGNGP GSFQPMNINY GLLPPLEAPK VDEDGKKIPL
460 470
KERGRAKKRL MSLRALKDLD AWMA
Length:474
Mass (Da):51,368
Last modified:June 1, 2001 - v1
Checksum:iE0E12FDC7887FF1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK24567.1.
PIRiC87571.
RefSeqiNP_421399.1. NC_002696.2.
WP_010920452.1. NC_002696.2.

Genome annotation databases

EnsemblBacteriaiAAK24567; AAK24567; CC_2598.
GeneIDi943599.
KEGGiccr:CC_2598.
PATRICi21302222. VBICauCre124313_2612.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA. Translation: AAK24567.1.
PIRiC87571.
RefSeqiNP_421399.1. NC_002696.2.
WP_010920452.1. NC_002696.2.

3D structure databases

ProteinModelPortaliQ9A566.
SMRiQ9A566. Positions 10-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190650.CC_2598.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK24567; AAK24567; CC_2598.
GeneIDi943599.
KEGGiccr:CC_2598.
PATRICi21302222. VBICauCre124313_2612.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.

Enzyme and pathway databases

BioCyciCAULO:CC2598-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR016040. NAD(P)-bd_dom.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_CAUCR
AccessioniPrimary (citable) accession number: Q9A566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.