Reviewed, UniProtKB/Swiss-Prot Q9A4J7 (CARA_CAUCR)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	CC_2834

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112265
Regions
	Domain	202 – 388	187	Glutamine amidotransferase type-1
	Region	1 – 198	198	CPSase HAMAP MF_01209
Sites
	Active site	279	1	Nucleophile By similarity
	Active site	363	1	By similarity
	Active site	365	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9A4J7-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 013EBC6EC91BE0EA	FASTA	388	40,701
	10 20 30 40 50 60 MSQDLLPGVT GVLALADGAI LQGVGCGAVG DAVGEVCFNT AMTGYQEILT DPSYMAQIVA 70 80 90 100 110 120 FTFPHVGNVG VNGEDVEQIT GVAETAARGA LFRDVPTVQA NWRADGDFDA WMKARGVVGL 130 140 150 160 170 180 AGVDTRALTR KIRETGMPHG VIAHSPDGQF DLAALVAKAK AWAGLEGLDL AKDASTTQTF 190 200 210 220 230 240 TWDEGLWSWP EGYAKLDKPK YEVVVIDYGV KRNILRALAH VGARATVVPA STSAEDILAR 250 260 270 280 290 300 NPDGVMLSNG PGDPAATGQY AVPEIQKLVA SGKPVFGICL GHQMLALALG AKTVKMEQGH 310 320 330 340 350 360 HGANHPVKDL TTGKVEIVSM NHGFTVDTAS LPAPVKETHV SLFDGTNAGI ALDGKPVFSV 370 380 QHHPEASPGP TDSLYLFERF AGLMDAAK

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK24798.1.
PIR	B87600.
RefSeq	NP_421630.1.
3D structure databases
SMR	Q9A4J7. Positions 10-388.
ModBase	Search...
Genome annotation databases
GeneID	943889.
GenomeReviews	Gene locus CC_2834 in contig AE005673_GR.
KEGG	ccr:CC_2834.
NMPDR	fig|190650.1.peg.2810.
TIGR	CC_2834.
Phylogenomic databases
HOGENOM	HBG286341.
OMA	FTYPELG.
Enzyme and pathway databases
BRENDA	6.3.5.5. 2191.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CAUCR

Accession

Primary (citable) accession number: Q9A4J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)