Q9A4E8 (T23O_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	CC_2886

Organism

Caulobacter crescentus (Caulobacter vibrioides)

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Tryptophan 2,3-dioxygenase		PRO_0000360113
Regions
	Region	7 – 11	5	Substrate binding By similarity
	Region	32 – 36	5	Substrate binding By similarity
Sites
	Metal binding	221	1	Iron (heme axial ligand) By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	Heme By similarity
	Binding site	235	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9A4E8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 17220BFF32FBA370	FASTA	263	29,919
	10 20 30 40 50 60 MAQDMTYARY LALDELLSAQ KPLSDRHDEL LFIVIHQTKE LWLKEILHEV ALALKLIAGG 70 80 90 100 110 120 DVEPAYKALA RVSRIQTVMT LSWDILATMT PADYLSFRDD LGTSSGFQSH QFRALEYLLG 130 140 150 160 170 180 LKDQSFLKFH TERPEALAML KSALESPSLY DVAIAQLPRH GLTVPDAALH RDFSQTYVPS 190 200 210 220 230 240 PEVEAAWLEV YRDPKRYWEL YQLAEKLVDL DDALVTWRHK HVLTVERIIG GRPGTGGTDG 250 260 VGYLASTLRR RAFPELWSLR TKL

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK24850.1.
PIR	F87606.
RefSeq	NP_421682.1. NC_002696.2.
3D structure databases
HSSP	HSSP built from PDB template 1YW0 based on UniProtKB Q8PDA8.
ProteinModelPortal	Q9A4E8.
SMR	Q9A4E8. Positions 3-263.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	940572.
GenomeReviews	Gene locus CC_2886 in contig AE005673_GR.
KEGG	ccr:CC_2886.
NMPDR	fig|190650.1.peg.2862.
PATRIC	21302792. VBICauCre124313_2890.
TIGR	CC_2886.
Phylogenomic databases
HOGENOM	HBG647485.
OMA	KLLVDQV.
ProtClustDB	CLSK2301668.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_CAUCR

Accession

Primary (citable) accession number: Q9A4E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program