Reviewed, UniProtKB/Swiss-Prot Q9A4C3 (NADB_CAUCR)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	CC_2913

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2).
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	L-aspartate oxidase		PRO_0000184381
Regions
	Nucleotide binding	15 – 29	15	FAD Potential
Sites
	Active site	236	1	By similarity
	Active site	255	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9A4C3-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 46B565ABC80B5EAF	FASTA	511	51,598
	10 20 30 40 50 60 MTQYRITFEG PVILGAGLAG LTAALSATTG AAKTALVLSP TPLASGCSSA WAQGGMAAAL 70 80 90 100 110 120 SGDDSPALHA ADTIAAGAGL CDPQAVDLLT REGPQAVRDL AALGAPFDRK ADDGFVLSLE 130 140 150 160 170 180 AAHSAARVAR VGGDGAGAAI MAAVIAAVRA TPGIEVRENA RARRLLQDAN GRVVGVLADV 190 200 210 220 230 240 DGALVEIRST AVILATGGVG GLYAVTTTPA QVRGEGLGLA ALAGAMIADP EFVQFHPTAI 250 260 270 280 290 300 DIGRDPAPLA TEALRGEGAI LRNADGKAFM ADYHPAKELA PRDVVARALH AERAAGRGAF 310 320 330 340 350 360 LDATAAVGAH FPHEFPAVFE ACMSAGIDPR RQMIPVTPAV HYHMGGVATD LDGRASLPGL 370 380 390 400 410 420 YAAGECASTG VQGANRLASN SLLEAAVFGA RAGRAAAAEG ATGGPPVSLE PLPDLPDAAL 430 440 450 460 470 480 QGLRKAMSRD AGVIRDADGL TRLLGEIETL EAGHGQGPIL VAARLIVTAA LAREESRGGH 490 500 510 CRIDFPATDP VGVRTFVTLD GREPGLRYAA E

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK24875.1.
PIR	G87609.
RefSeq	NP_421707.1.
3D structure databases
HSSP	HSSP built from PDB template 1D4D based on UniProtKB P83223.
ModBase	Search...
Genome annotation databases
GeneID	942841.
GenomeReviews	Gene locus CC_2913 in contig AE005673_GR.
KEGG	ccr:CC_2913.
NMPDR	fig|190650.1.peg.2887.
TIGR	CC_2913.
Phylogenomic databases
HOGENOM	Q9A4C3.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

NADB_CAUCR

Accession

Primary (citable) accession number: Q9A4C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	June 1, 2001
Last modified:	November 4, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)