Q9A3J3 (DNAE2_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	CC_3211

Organism

Caulobacter crescentus (Caulobacter vibrioides)

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	1083 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). Along with imuA and imuB is required for the error-prone processing of DNA lesions By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1083	1083	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103375

Sequences

	Sequence		Length	Mass (Da)
	Q9A3J3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 124E5F216A555E64	FASTA	1,083	120,800
	10 20 30 40 50 60 MRPPVYAELQ ATTNFSFLRG ASHAEELALT AEALGLTAIG IVDRNSLAGV VRAWTAAKRR 70 80 90 100 110 120 SIRVLTGCRL DFMDGAPSLL CYPTDREAFG RLTRLLTIGQ LRAEKGECHL TWRDFLDHSE 130 140 150 160 170 180 GQLGLIVPPR VLDDSFEQHL TRMAGDLRGR SWLAASRAYA ARDLQRLARL ESLGRTSGAP 190 200 210 220 230 240 IVATNDVLYH GPERRPLQDV ISCVREHCAI QEAGFRLEAN AERHIKSPEE MARLFDRWPR 250 260 270 280 290 300 AVERTVEIVE RIGFDLKDIR EQYPDEPVPP GKTAMQHLTD LTWKGAAWRY PNGVSPKVTA 310 320 330 340 350 360 QIQEELRLIE KMDYPNYFIT VHDIVREARS MGILCQGRGS AANSSVCFCL GVTAIDPTEH 370 380 390 400 410 420 RLLFTRFISE NRGEPPDIDV DFEHDRREEV MQYVFQRYGR AYAAICGTVI HYRPRSAIRD 430 440 450 460 470 480 VGKALGLTED VTSLLAGTVW GSWGDGLPED HLRNAGLDPK APEIARAVGL ANDLIGFPRH 490 500 510 520 530 540 LSQHVGGFVL TKRRLDETVP IGKAAMKDRT FIEWDKDDID SLGLMKVDIL ALGMLHAIQR 550 560 570 580 590 600 AMTMLREDHG QDWLKDLADI PKEVPGVYDM LCAADSVGVF QVESRAQMSM LPRLRPREFY 610 620 630 640 650 660 DLVIQVAIVR PGPIQGDMVH PYLKRRNKEE PVDWPKPSPE HGPPDELQEI LGKTFGVPLF 670 680 690 700 710 720 QEQAMSLAIE AAKFTPDEAD GLRKAMATFR NLGSPDAYRN KFIEGMVGRG YERAFAERCF 730 740 750 760 770 780 KQIEGFSHYG FPESHAASFA KLVYVSAWIK WAWPDVFCAA LINAQPMGFY QPAQLVRDAR 790 800 810 820 830 840 EHGVEVLPPD ILTSDWDCTL APISTGFRPP RVRHDKVACQ ETRPRWKAVR LGFRQIKGLR 850 860 870 880 890 900 ETLDIPPLLK ARGEGARTPA EFAQGGVPQK ALELLAEADA FASVGLSRRE ALWAVKGLKG 910 920 930 940 950 960 EHKAPVQAPL LAGLPLFEER VALPAMAATQ EVAEDYRTTS LSLKAHPIGF YRSMLAARGV 970 980 990 1000 1010 1020 VPAERLLSLK DGARVSVAGL VLIRQRPGTA KGVVFVTLED ETGVANAVVW KDRFDAARNV 1030 1040 1050 1060 1070 1080 VMTASFLIVH GRVQRADNVI HVVAERFTDL SAELSSLRDE PGAPAPRIRQ KVSGRLLRSR DFH

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK25173.1.
PIR	A87647.
RefSeq	NP_422005.1. NC_002696.2.
3D structure databases
ProteinModelPortal	Q9A3J3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	940962.
GenomeReviews	Gene locus CC_3211 in contig AE005673_GR.
KEGG	ccr:CC_3211.
NMPDR	fig|190650.1.peg.3185.
PATRIC	21303450. VBICauCre124313_3217.
TIGR	CC_3211.
Phylogenomic databases
HOGENOM	HBG734490.
OMA	LATNRNG.
ProtClustDB	CLSK891351.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_CAUCR

Accession

Primary (citable) accession number: Q9A3J3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program