Reviewed, UniProtKB/Swiss-Prot Q9A2X6 (PROA_CAUCR)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	CC_3430

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189711

Sequences

	Sequence		Length	Mass (Da)
	Q9A2X6-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 01D77D46F6EA38DC	FASTA	413	42,493
	10 20 30 40 50 60 MAGMGRTARE GARALRLATP EQRTAAIQAM AVAIREDADA ILTANAKDLA RAEANGVSGP 70 80 90 100 110 120 MLDRLALDAA RLEGVAAGVE AVAAVPDPVG VATSRWTRPN GLDIARVRTP IGVIAMIYES 130 140 150 160 170 180 RPNVTADAAA LCVRSGNAVI LRGGSECIHS NLAIHAAIAR GLQKAGLPTA AVQAVKTPDR 190 200 210 220 230 240 AAVGMILAGL DRTIDLIIPR GGKSLVARVQ AEARAPVLGH LEGLNHVFVH AAADLKKAVE 250 260 270 280 290 300 VVVNAKLRRV SVCGSAETLL IDQAAADKLL PPIADALIKA GCELRGDAAA RAIEPTMKKA 310 320 330 340 350 360 TVEDWTTEYL APILSVAVVD GVAGAARHIA SYGSGHTDAI ITEDAAAAEA FIAEVDSAIV 370 380 390 400 410 LVNASTQFAD GGEFGFGAEI GIATDKLHAR GPVGAEQLTT FKYVVRGTGQ TRP

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK25392.1.
PIR	D87674.
RefSeq	NP_422224.1.
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Genome annotation databases
GeneID	940829.
GenomeReviews	Gene locus CC_3430 in contig AE005673_GR.
KEGG	ccr:CC_3430.
NMPDR	fig|190650.1.peg.3404.
TIGR	CC_3430.
Phylogenomic databases
HOGENOM	Q9A2X6.
OMA	Q9A2X6. QYPAACN.
Enzyme and pathway databases
BRENDA	1.2.1.41. 2191.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CAUCR

Accession

Primary (citable) accession number: Q9A2X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)