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Q9A2N5 (BIOB_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:CC_3521
OrganismCaulobacter crescentus (strain ATCC 19089 / CB15) [Reference proteome] [HAMAP]
Taxonomic identifier190650 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Biotin synthase HAMAP-Rule MF_01694
PRO_0000381292

Sites

Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1021Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1331Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1931Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2701Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9A2N5 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: E5D55FDCF19F3CB4

FASTA34136,566
        10         20         30         40         50         60 
MSEITASPRH DWTLAEVEAL FALPFMELVF QAANVHRAHF DPSEIQLSQL LSVKTGGCAE 

        70         80         90        100        110        120 
NCGYCSQSAH FKTGLKADKL MAADDVVAKA RAARDGGAQR FCMGAAWREL KDRDLPKLTE 

       130        140        150        160        170        180 
MIGEVKALGL ETCATLGMLT ADQAKALKAA GLDYYNHNLD TGPDYYKDVV TTRTYQERLD 

       190        200        210        220        230        240 
TLAHVRDAGM STCCGGIVGM GEQRRDRAGL LHQLATLPAH PDSLPINGLV PISGTPLGDK 

       250        260        270        280        290        300 
VLSEGKAIDA IEFVRTIAVA RIVCPKSMVR LSAGREGMSR ELQALCFMAG ANSIFVGGKL 

       310        320        330        340 
LTTPLPGQDE DSQLFQDLDL KPMGGAVRVE AKADAAMVAA E 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005673 Genomic DNA. Translation: AAK25483.1.
PIRG87685.
RefSeqNP_422315.1. NC_002696.2.

3D structure databases

ProteinModelPortalQ9A2N5.
SMRQ9A2N5. Positions 9-322.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK25483; AAK25483; CC_3521.
GeneID940770.
KEGGccr:CC_3521.
PATRIC21304074. VBICauCre124313_3529.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2301681.

Enzyme and pathway databases

BioCycCAULO:CC3521-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CAUCR
AccessionPrimary (citable) accession number: Q9A2N5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways