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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotation

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.Curated

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (CC_0843)
  2. Aspartate-semialdehyde dehydrogenase (asd), Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADUniRule annotation1
Binding sitei38NADPUniRule annotation1
Active sitei144Proton donor/acceptorUniRule annotation1
Binding sitei145SubstrateUniRule annotation1
Active sitei148Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16NAD(P)UniRule annotation6
Nucleotide bindingi86 – 88NAD(P)UniRule annotation3
Nucleotide bindingi110 – 113NAD(P)UniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandNAD, NADP

Enzyme and pathway databases

BioCyciCAULO:CC3550-MONOMER
UniPathwayiUPA00034; UER00018

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotation (EC:1.17.1.8UniRule annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:CC_3550
OrganismiCaulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus)
Taxonomic identifieri190650 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
Proteomesi
  • UP000001816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001414251 – 2574-hydroxy-tetrahydrodipicolinate reductaseAdd BLAST257

Interactioni

Protein-protein interaction databases

STRINGi190650.CC_3550

Structurei

3D structure databases

ProteinModelPortaliQ9A2L1
SMRiQ9A2L1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 155Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DUK Bacteria
COG0289 LUCA
HOGENOMiHOG000227153
KOiK00215
OMAiEHEVIFA

Family and domain databases

HAMAPiMF_00102 DapB, 1 hit
InterProiView protein in InterPro
IPR022663 DapB_C
IPR000846 DapB_N
IPR022664 DapB_N_CS
IPR023940 DHDPR_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR20836 PTHR20836, 1 hit
PfamiView protein in Pfam
PF05173 DapB_C, 1 hit
PF01113 DapB_N, 1 hit
PIRSFiPIRSF000161 DHPR, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits
TIGRFAMsiTIGR00036 dapB, 1 hit
PROSITEiView protein in PROSITE
PS01298 DAPB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9A2L1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPVKIAIA GANGRMGRAV AQALEGRSDA VVAARFERPG DAGEGLVARE
60 70 80 90 100
TALAAAEAVI DFTLPEASVA LAEEAAANGG PALVIGSTGF SDEQLDRIDA
110 120 130 140 150
AATKIVIVRS GNYSLGVNML MGLVRQAAAA LPAQDWDIEV FEAHHKRKID
160 170 180 190 200
APSGTALMLG EAAAEGRGIN LAKVSDRGRD GVTGPRKDGD IGFSVVRGGG
210 220 230 240 250
IIGEHSVIFA GESESLTLSH SAIDRGLFAR GAIAAAVWVK GKPPGLYDMQ

DVLGFKK
Length:257
Mass (Da):26,425
Last modified:June 1, 2001 - v1
Checksum:iB8AD3BB269A47AE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005673 Genomic DNA Translation: AAK25512.1
PIRiD87689
RefSeqiNP_422344.1, NC_002696.2
WP_010921379.1, NC_002696.2

Genome annotation databases

EnsemblBacteriaiAAK25512; AAK25512; CC_3550
GeneIDi943731
KEGGiccr:CC_3550
PATRICifig|190650.5.peg.3553

Similar proteinsi

Entry informationi

Entry nameiDAPB_CAUVC
AccessioniPrimary (citable) accession number: Q9A2L1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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