Q9A253 (DUT_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	CC_3713

Organism

Caulobacter crescentus (strain ATCC 19089 / CB15) [Reference proteome] [HAMAP]

Taxonomic identifier

190650 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter › Caulobacter crescentus

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_0000182843
Regions
	Region	75 – 77	3	Substrate binding By similarity
	Region	92 – 94	3	Substrate binding By similarity
Sites
	Binding site	88	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9A253 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 534EC085942C4118	FASTA	155	16,215
	10 20 30 40 50 60 MSAIPAGLAI RFKRWEGNAD LPVPAYATVG AAGFDLRAHV PDDAPIVLKP GARCMAPTGF 70 80 90 100 110 120 SVAIPDGYEM QVRPRSGLAL KNGVTVVNAP GTVDSDYRGQ VCVLLINLGE EDFTIRRGDR 130 140 150 IAQGVIAAAP QWPLVEVEDL DATERGAGGF GSTGV

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005673 Genomic DNA. Translation: AAK25675.1.
PIR	G87709.
RefSeq	NP_422507.1. NC_002696.2.
3D structure databases
ProteinModelPortal	Q9A253.
SMR	Q9A253. Positions 14-136.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK25675; AAK25675; CC_3713.
GeneID	940629.
KEGG	ccr:CC_3713.
PATRIC	21304462. VBICauCre124313_3714.
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028966.
KO	K01520.
OMA	ILPRSGM.
ProtClustDB	CLSK2461482.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_CAUCR

Accession

Primary (citable) accession number: Q9A253

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program