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Q9A217 (SYL_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CC_3749
OrganismCaulobacter crescentus (strain ATCC 19089 / CB15) [Reference proteome] [HAMAP]
Taxonomic identifier190650 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151995

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif623 – 6275"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6261ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9A217 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: C546C7C0BD7DB374

FASTA86195,536
        10         20         30         40         50         60 
MARYNPKDTE PKWREAWAKA DVFKTGEIND GRPKYYVLEM FPYPSGRIHM GHVRNYAMGD 

        70         80         90        100        110        120 
VVARYKRAQG FNVLHPMGWD AFGMPAENAA MERGVHPKGW TYDNIAAMRE QLKSLGISVD 

       130        140        150        160        170        180 
WSREFATCDP EYYGKQQAWF LRLLKRGLVY RKEASVNWDP VDMTVLANEQ VIDGKGWRSG 

       190        200        210        220        230        240 
AVVEKRKLTQ WFLRITDYAD ALIDGLKTLD RWPDKVRLMQ ENWIGRSKGL RFKFQFDGEA 

       250        260        270        280        290        300 
PDGMAEGLEV YTTRPDTLFG ASFVGIAPEH PLAEQLAAAN PQIQTFIADC RKGGTSEAEI 

       310        320        330        340        350        360 
ESAEKLGYDT GLRVKHPLDP SITLPVWIAN FILMDYGTGA IFACPAHDQR DLDFARKYDL 

       370        380        390        400        410        420 
PVLPVVLPNG EDPATFTVGK EAYVGPGKIF NSKFLDGLDV EAAKAEAIAR IEAANQGQGA 

       430        440        450        460        470        480 
TVYRLRDWGV SRQRYWGCPI PVIHCEACGV VPVPEDQLPV ALPDDVTFDK PGNPLLRHPT 

       490        500        510        520        530        540 
WRHTTCPSCG GKAERETDTL DTFIDSSWYF ARFADTQAAE PVGKDAADHW LPVDQYIGGV 

       550        560        570        580        590        600 
EHAILHLLYA RFITRALKDE GLLSVEEPFA GLFTQGMVTH EAYKNEAGEW VEPSDVVITT 

       610        620        630        640        650        660 
EGSTRTAKHA KTGAPIIIGD IEKMSKSKKN VVAPEDIFEA YGVDSARLFV MSDSPPERDV 

       670        680        690        700        710        720 
QWTNSGVEGS WRFTHRLWNE FDSQPAGDFA HDDSDEAALA LRKAAHKLIG FVTDSIEGFR 

       730        740        750        760        770        780 
FNSGVARLYE FLNALKAAPA EGASQAVLAA RAEALNILAR LVAPFTPHLA EEAWARMGGE 

       790        800        810        820        830        840 
GMVVDAPWPK ADPALAADDE RVLPIQINGK RRGEIKVKAG APDDEVTKIA LADPNVMAHL 

       850        860 
EGLTVRKVIV VKDRIVNIVA N 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005673 Genomic DNA. Translation: AAK25711.1.
PIRC87714.
RefSeqNP_422543.1. NC_002696.2.

3D structure databases

ProteinModelPortalQ9A217.
SMRQ9A217. Positions 3-859.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9A217.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK25711; AAK25711; CC_3749.
GeneID940606.
KEGGccr:CC_3749.
PATRIC21304536. VBICauCre124313_3751.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCAULO:CC3749-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CAUCR
AccessionPrimary (citable) accession number: Q9A217
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries