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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi284Magnesium or manganeseUniRule annotation1
Metal bindingi286Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 195ATPUniRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:SPy_0032, M5005_Spy0029
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000750 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514891 – 421Phosphoribosylamine--glycine ligaseAdd BLAST421

Proteomic databases

PaxDbiQ9A1Y7
PRIDEiQ9A1Y7

Interactioni

Protein-protein interaction databases

STRINGi160490.SPy_0032

Structurei

3D structure databases

ProteinModelPortaliQ9A1Y7
SMRiQ9A1Y7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 314ATP-graspUniRule annotationAdd BLAST207

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C12 Bacteria
COG0151 LUCA
HOGENOMiHOG000033463
KOiK01945
OMAiKATVCKY

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9A1Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLVVGSGG REHAIAKKLL ASKGVDQVFV APGNDGMTLD GLDLVNIVVS
60 70 80 90 100
EHSRLIAFAK ENEISWAFIG PDDALAAGIV DDFNSAGLRA FGPTKAAAEL
110 120 130 140 150
EWSKDFAKEI MVKYNVPTAA YGTFSDFEKA KAYIEEQGAP IVVKADGLAL
160 170 180 190 200
GKGVVVAETV EQAVEAAQEM LLDNKFGDSG ARVVIEEFLD GEEFSLFAFA
210 220 230 240 250
NGDKFYIMPT AQDHKRAFDG DKGPNTGGMG AYAPVPHLPQ SVVDTAVEMI
260 270 280 290 300
VRPVLEGMVA EGRPYLGVLY VGLILTADGP KVIEFNSRFG DPETQIILPR
310 320 330 340 350
LTSDFAQNID DIMMGIEPYI TWQKDGVTLG VVVASEGYPF DYEKGVPLPE
360 370 380 390 400
KTDGDIITYY AGVKFSENSE LLLSNGGRVY MLVTTEDSVK AGQDKIYTQL
410 420
AQQDTTGLFY RNDIGSKAIR E
Length:421
Mass (Da):45,481
Last modified:June 1, 2001 - v1
Checksum:i966A3324767E589E
GO

Sequence cautioni

The sequence AAZ50648 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA Translation: AAK33169.1
CP000017 Genomic DNA Translation: AAZ50648.1 Different initiation.
RefSeqiNP_268447.1, NC_002737.2

Genome annotation databases

EnsemblBacteriaiAAK33169; AAK33169; SPy_0032
AAZ50648; AAZ50648; M5005_Spy0029
GeneIDi900390
KEGGispy:SPy_0032
spz:M5005_Spy0029
PATRICifig|160490.10.peg.30

Similar proteinsi

Entry informationi

Entry nameiPUR2_STRP1
AccessioniPrimary (citable) accession number: Q9A1Y7
Secondary accession number(s): Q491S0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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