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Q9A1Y7 (PUR2_STRP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:SPy_0032, M5005_Spy0029
OrganismStreptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]
Taxonomic identifier301447 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAZ50648.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151489

Regions

Domain108 – 314207ATP-grasp
Nucleotide binding134 – 19562ATP By similarity

Sites

Metal binding2841Magnesium or manganese By similarity
Metal binding2861Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9A1Y7 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 966A3324767E589E

FASTA42145,481
        10         20         30         40         50         60 
MKLLVVGSGG REHAIAKKLL ASKGVDQVFV APGNDGMTLD GLDLVNIVVS EHSRLIAFAK 

        70         80         90        100        110        120 
ENEISWAFIG PDDALAAGIV DDFNSAGLRA FGPTKAAAEL EWSKDFAKEI MVKYNVPTAA 

       130        140        150        160        170        180 
YGTFSDFEKA KAYIEEQGAP IVVKADGLAL GKGVVVAETV EQAVEAAQEM LLDNKFGDSG 

       190        200        210        220        230        240 
ARVVIEEFLD GEEFSLFAFA NGDKFYIMPT AQDHKRAFDG DKGPNTGGMG AYAPVPHLPQ 

       250        260        270        280        290        300 
SVVDTAVEMI VRPVLEGMVA EGRPYLGVLY VGLILTADGP KVIEFNSRFG DPETQIILPR 

       310        320        330        340        350        360 
LTSDFAQNID DIMMGIEPYI TWQKDGVTLG VVVASEGYPF DYEKGVPLPE KTDGDIITYY 

       370        380        390        400        410        420 
AGVKFSENSE LLLSNGGRVY MLVTTEDSVK AGQDKIYTQL AQQDTTGLFY RNDIGSKAIR 


E 

« Hide

References

[1]"Complete genome sequence of an M1 strain of Streptococcus pyogenes."
Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. expand/collapse author list , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E.
Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700294 / SF370 / Serotype M1.
[2]"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004092 Genomic DNA. Translation: AAK33169.1.
CP000017 Genomic DNA. Translation: AAZ50648.1. Different initiation.
RefSeqNP_268447.1. NC_002737.1.
YP_281393.1. NC_007297.1.

3D structure databases

ProteinModelPortalQ9A1Y7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160490.SPy_0032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK33169; AAK33169; SPy_0032.
AAZ50648; AAZ50648; M5005_Spy0029.
GeneID3572913.
900390.
KEGGspy:SPy_0032.
spz:M5005_Spy_0029.
PATRIC19713876. VBIStrPyo79812_0030.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMACIAGLKN.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycSPYO160490:GJ81-51-MONOMER.
SPYO293653:GHFC-63-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_STRP1
AccessionPrimary (citable) accession number: Q9A1Y7
Secondary accession number(s): Q491S0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways