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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei613 – 6131ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-174-MONOMER.
SPYO293653:GHFC-197-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:SPy_0173, M5005_Spy0147
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000750 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 833833Leucine--tRNA ligasePRO_0000152097Add
BLAST

Proteomic databases

PaxDbiQ9A1P0.

Interactioni

Protein-protein interaction databases

STRINGi160490.SPy_0173.

Structurei

3D structure databases

ProteinModelPortaliQ9A1P0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5212"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9A1P0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFYDHTAIE PKWQAFWADN HTFKTGTDAS KPKFYALDMF PYPSGAGLHV
60 70 80 90 100
GHPEGYTATD ILSRFKRAQG HNILHPMGWD AFGLPAEQYA MDTGNDPAEF
110 120 130 140 150
TAENIANFKR QINALGFSYD WDREVNTTDP NYYKWTQWIF TKLYEKGLAY
160 170 180 190 200
EAEVPVNWVE ELGTAIANEE VLPDGTSERG GYPVVRKPMR QWMLKITAYA
210 220 230 240 250
ERLLEDLEEV DWPESIKDMQ RNWIGKSTGA NVTFKVKDTD KDFTVFTTRP
260 270 280 290 300
DTLFGATYAV LAPEHALVDA ITTADQAEAV AKYKRQASLK SDLARTDLAK
310 320 330 340 350
EKTGVWTGAY AINPVNGNEM PVWIADYVLA SYGTGAIMAV PAHDERDWEF
360 370 380 390 400
AKQFKLDIIP VLEGGNVEEA AFTEDGLHIN SGFLDGLDKA SAIAKMVEWL
410 420 430 440 450
EAEGVGNEKV TYRLRDWLFS RQRYWGEPIP IIHWEDGTST AVPESELPLV
460 470 480 490 500
LPVTKDIRPS GTGESPLANV TDWLEVTRED GVKGRRETNT MPQWAGSSWY
510 520 530 540 550
YLRYIDPHNT EKLADEELLK QWLPVDIYVG GAEHAVLHLL YARFWHKVLY
560 570 580 590 600
DLGVVPTKEP FQKLFNQGMI LGTSYRDSRG ALVATDKVEK RDGSFFHVET
610 620 630 640 650
GEELEQAPAK MSKSLKNVVN PDDVVEQYGA DTLRVYEMFM GPLDASIAWS
660 670 680 690 700
EEGLEGSRKF LDRVYRLITT KEITEENSGA LDKVYNETVK AVTEQVDQMK
710 720 730 740 750
FNTAIAQLMV FVNAANKEDK LFSDYAKGFV QLIAPFAPHL GEELWQALTA
760 770 780 790 800
SGESISYVPW PSYDESKLVE NDVEIVVQIK GKVKAKLVVA KDLSREELQE
810 820 830
VALANEKVQA EIAGKDIIKV IAVPNKLVNI VIK
Length:833
Mass (Da):93,816
Last modified:June 1, 2001 - v1
Checksum:i59168EADE9B9FFB3
GO

Sequence cautioni

The sequence AAZ50766.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731I → V in AAZ50766 (PubMed:16088826).Curated
Sequence conflicti274 – 2741A → S in AAZ50766 (PubMed:16088826).Curated
Sequence conflicti282 – 2821K → D in AAZ50766 (PubMed:16088826).Curated
Sequence conflicti309 – 3091A → S in AAZ50766 (PubMed:16088826).Curated
Sequence conflicti318 – 3181N → K in AAZ50766 (PubMed:16088826).Curated
Sequence conflicti355 – 3551K → N in AAZ50766 (PubMed:16088826).Curated
Sequence conflicti382 – 3821G → D in AAZ50766 (PubMed:16088826).Curated
Sequence conflicti409 – 4091K → N in AAZ50766 (PubMed:16088826).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK33273.1.
CP000017 Genomic DNA. Translation: AAZ50766.1. Different initiation.
RefSeqiNP_268552.1. NC_002737.1.

Genome annotation databases

EnsemblBacteriaiAAK33273; AAK33273; SPy_0173.
AAZ50766; AAZ50766; M5005_Spy0147.
GeneIDi900496.
KEGGispy:SPy_0173.
spz:M5005_Spy_0147.
PATRICi19714151. VBIStrPyo79812_0151.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK33273.1.
CP000017 Genomic DNA. Translation: AAZ50766.1. Different initiation.
RefSeqiNP_268552.1. NC_002737.1.

3D structure databases

ProteinModelPortaliQ9A1P0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160490.SPy_0173.

Proteomic databases

PaxDbiQ9A1P0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK33273; AAK33273; SPy_0173.
AAZ50766; AAZ50766; M5005_Spy0147.
GeneIDi900496.
KEGGispy:SPy_0173.
spz:M5005_Spy_0147.
PATRICi19714151. VBIStrPyo79812_0151.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-174-MONOMER.
SPYO293653:GHFC-197-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700294 / SF370 / Serotype M1.
  2. "Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
    Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
    J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Entry informationi

Entry nameiSYL_STRP1
AccessioniPrimary (citable) accession number: Q9A1P0
Secondary accession number(s): Q491F2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.