Q9A155 (FTSK_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA translocase FtsK

Gene names

Name:	ftsK
Ordered Locus Names:	SPy_0458, M5005_Spy0372

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the difSL recombination site, which is located within the replication terminus region. Required for activation of the XerS recombinase, allowing activation of chromosome unlinking by recombination By similarity.
Subunit structure	Homohexamer. Forms a ring that surrounds DNA By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Note: Located at the septum By similarity.
Domain	Consists of an N-terminal domain, which is sufficient for the localization to the septal ring and is required for cell division, followed by a linker domain, and a C-terminal domain, which forms the translocation motor involved in chromosome segregation. The C-terminal domain can be further subdivided into alpha, beta and gamma subdomains. The alpha and beta subdomains form the DNA pump, and the gamma subdomain is a regulatory subdomain By similarity.
Sequence similarities	Belongs to the FtsK/SpoIIIE/SftA family. Contains 1 FtsK domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 801	801	DNA translocase FtsK		PRO_0000098308
Regions
	Transmembrane	31 – 53	23	Helical; Potential
	Transmembrane	58 – 80	23	Helical; Potential
	Transmembrane	89 – 111	23	Helical; Potential
	Transmembrane	131 – 150	20	Helical; Potential
	Transmembrane	155 – 177	23	Helical; Potential
	Topological domain	178 – 801	624	Cytoplasmic Potential
	Domain	464 – 660	197	FtsK
	Nucleotide binding	484 – 489	6	ATP By similarity
Experimental info
	Sequence conflict	711	1	T → A in AAZ50990. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9A155 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 2AEE3CF9E6A93495	FASTA	801	89,328
	10 20 30 40 50 60 MVKRNQRKKS APKKRLTKAE VEKQRAIKRM ILSVLMALLL IFAMLRLGVF GVTTYNMIRF 70 80 90 100 110 120 LVGSLAYPFM FAWLIYLFCF KWLRQKDGMI AGVVIAFLGL LVEWHAFLFA MPRMLDQDIF 130 140 150 160 170 180 LGTARLITRD LLALRVTEFV GGGMLGALLY KPIAFLFSNI GSYFIGFLFI LLGLFLMTPW 190 200 210 220 230 240 DIYDVSHFVK EAVDKLAVAY QENKEKRFIK REEHRLQAEK EALEKQAQEE EKRLAELTVD 250 260 270 280 290 300 PETGEIVEDS QSQVSYDLAE DMTKEPEILA YDSHLKDDET SLFDQEDLAY AHEEIGAYDS 310 320 330 340 350 360 LSALASSEDE MDMDEPVEVD FTPKTHLLYK LPTIDLFAPD KPKNQSKEKN LVRKNIKVLE 370 380 390 400 410 420 DTFQSFGIDV KVERAEIGPS VTKYEIKPAV GVRVNRISNL ADDLALALAA KDVRIEAPIP 430 440 450 460 470 480 GKSLIGIEVP NSEIATVSFR ELWEQSDANP ENLLEVPLGK AVNGNARSFN LARMPHLLVA 490 500 510 520 530 540 GSTGSGKSVA VNGIISSILM KARPDQVKFM MIDPKMVELS VYNDIPHLLI PVVTNPRKAS 550 560 570 580 590 600 KALQKVVDEM ENRYELFSKI GVRNIAGYNT KVEEFNASSE QKQIPLPLIV VIVDELADLM 610 620 630 640 650 660 MVASKEVEDA IIRLGQKARA AGIHMILATQ RPSVDVISGL IKANVPSRMA FAVSSGTDSR 670 680 690 700 710 720 TILDENGAEK LLGRGDMLFK PIDENHPVRL QGSFISDDDV ERIVNFIKDQ TEADYDDAFD 730 740 750 760 770 780 PGEVSDNDPG FSGNGGAAEG DPLFEEAKAL VLETQKASAS MIQRRLSVGF NRATRLMDEL 790 800 EEAGVIGPAE GTKPRKVLQT N

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK33472.1. CP000017 Genomic DNA. Translation: AAZ50990.1.
RefSeq	NP_268751.1. NC_002737.1. YP_281735.1. NC_007297.1.
3D structure databases
ProteinModelPortal	Q9A155.
ModBase	Search...
Protein-protein interaction databases
STRING	160490.SPy_0458.
Proteomic databases
PaxDb	Q9A155.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK33472; AAK33472; SPy_0458. AAZ50990; AAZ50990; M5005_Spy0372.
GeneID	3572544. 900700.
KEGG	spy:SPy_0458. spz:M5005_Spy_0372.
PATRIC	19714635. VBIStrPyo79812_0386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1674.
HOGENOM	HOG000010003.
KO	K03466.
OMA	FLMTPWD.
ProtClustDB	CLSK877051.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR002543. Cell_div_FtsK/SpoIIIE. IPR018541. DNA_translocase_Ftsk_gamma. [Graphical view]
Pfam	PF09397. Ftsk_gamma. 1 hit. PF01580. FtsK_SpoIIIE. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00843. Ftsk_gamma. 1 hit. [Graphical view]
PROSITE	PS50901. FTSK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTSK_STRP1

Accession

Primary (citable) accession number: Q9A155
Secondary accession number(s): Q490H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2003
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program