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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 9615-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei100 – 10015-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei102 – 10215-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation
Binding sitei126 – 1261OrotateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-740-MONOMER.
SPYO293653:GHFC-760-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:SPy_0901, M5005_Spy0704
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000750 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Orotate phosphoribosyltransferasePRO_0000110753Add
BLAST

Proteomic databases

PaxDbiQ9A076.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160490.SPy_0901.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1311Combined sources
Beta strandi16 – 194Combined sources
Helixi28 – 303Combined sources
Beta strandi32 – 376Combined sources
Helixi39 – 446Combined sources
Helixi46 – 6318Combined sources
Beta strandi69 – 735Combined sources
Turni74 – 774Combined sources
Helixi78 – 8811Combined sources
Beta strandi92 – 954Combined sources
Beta strandi107 – 1104Combined sources
Beta strandi117 – 12711Combined sources
Helixi129 – 14012Combined sources
Beta strandi144 – 15310Combined sources
Helixi157 – 16610Combined sources
Beta strandi170 – 1734Combined sources
Helixi176 – 18510Combined sources
Helixi191 – 20212Combined sources
Turni204 – 2063Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AEEX-ray1.95A/B1-209[»]
ProteinModelPortaliQ9A076.
SMRiQ9A076. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9A076.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 13095-phosphoribose 1-diphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9A076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLASQIATQ LLDIKAVYLK PEDPFTWASG IKSPIYTDNR VTLSYPKTRD
60 70 80 90 100
LIENGFVETI KAHFPEVEVI AGTATAGIPH GAIIADKMTL PFAYIRSKPK
110 120 130 140 150
DHGAGNQIEG RVLKGQKMVI IEDLISTGGS VLDAAAAASR EGADVLGVVA
160 170 180 190 200
IFTYELPKAS QNFKEAGIKL ITLSNYTELI AVAKLQGYIT NDGLHLLKKF

KEDQVNWQQ
Length:209
Mass (Da):22,743
Last modified:June 1, 2001 - v1
Checksum:i6113019D78A877AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK33818.1.
CP000017 Genomic DNA. Translation: AAZ51322.1.
RefSeqiNP_269097.1. NC_002737.2.

Genome annotation databases

EnsemblBacteriaiAAK33818; AAK33818; SPy_0901.
AAZ51322; AAZ51322; M5005_Spy0704.
GeneIDi901056.
KEGGispy:SPy_0901.
spz:M5005_Spy0704.
PATRICi19715412. VBIStrPyo79812_0774.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK33818.1.
CP000017 Genomic DNA. Translation: AAZ51322.1.
RefSeqiNP_269097.1. NC_002737.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AEEX-ray1.95A/B1-209[»]
ProteinModelPortaliQ9A076.
SMRiQ9A076. Positions 1-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160490.SPy_0901.

Proteomic databases

PaxDbiQ9A076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK33818; AAK33818; SPy_0901.
AAZ51322; AAZ51322; M5005_Spy0704.
GeneIDi901056.
KEGGispy:SPy_0901.
spz:M5005_Spy0704.
PATRICi19715412. VBIStrPyo79812_0774.

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciSPYO160490:GJ81-740-MONOMER.
SPYO293653:GHFC-760-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9A076.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_STRP1
AccessioniPrimary (citable) accession number: Q9A076
Secondary accession number(s): Q48Z96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.