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Protein

Orotate phosphoribosyltransferase

Gene

pyrE

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei965-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1005-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei1025-phosphoribose 1-diphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei126OrotateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferaseUniRule annotation (EC:2.4.2.10UniRule annotation)
Short name:
OPRTUniRule annotation
Short name:
OPRTaseUniRule annotation
Gene namesi
Name:pyrEUniRule annotation
Ordered Locus Names:SPy_0901, M5005_Spy0704
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000750 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001107531 – 209Orotate phosphoribosyltransferaseAdd BLAST209

Proteomic databases

PaxDbiQ9A076.
PRIDEiQ9A076.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160490.SPy_0901.

Structurei

Secondary structure

1209
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 13Combined sources11
Beta strandi16 – 19Combined sources4
Helixi28 – 30Combined sources3
Beta strandi32 – 37Combined sources6
Helixi39 – 44Combined sources6
Helixi46 – 63Combined sources18
Beta strandi69 – 73Combined sources5
Turni74 – 77Combined sources4
Helixi78 – 88Combined sources11
Beta strandi92 – 95Combined sources4
Beta strandi107 – 110Combined sources4
Beta strandi117 – 127Combined sources11
Helixi129 – 140Combined sources12
Beta strandi144 – 153Combined sources10
Helixi157 – 166Combined sources10
Beta strandi170 – 173Combined sources4
Helixi176 – 185Combined sources10
Helixi191 – 202Combined sources12
Turni204 – 206Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AEEX-ray1.95A/B1-209[»]
ProteinModelPortaliQ9A076.
SMRiQ9A076.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9A076.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 1305-phosphoribose 1-diphosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9A076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLASQIATQ LLDIKAVYLK PEDPFTWASG IKSPIYTDNR VTLSYPKTRD
60 70 80 90 100
LIENGFVETI KAHFPEVEVI AGTATAGIPH GAIIADKMTL PFAYIRSKPK
110 120 130 140 150
DHGAGNQIEG RVLKGQKMVI IEDLISTGGS VLDAAAAASR EGADVLGVVA
160 170 180 190 200
IFTYELPKAS QNFKEAGIKL ITLSNYTELI AVAKLQGYIT NDGLHLLKKF

KEDQVNWQQ
Length:209
Mass (Da):22,743
Last modified:June 1, 2001 - v1
Checksum:i6113019D78A877AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK33818.1.
CP000017 Genomic DNA. Translation: AAZ51322.1.
RefSeqiNP_269097.1. NC_002737.2.

Genome annotation databases

EnsemblBacteriaiAAK33818; AAK33818; SPy_0901.
AAZ51322; AAZ51322; M5005_Spy0704.
GeneIDi901056.
KEGGispy:SPy_0901.
spz:M5005_Spy0704.
PATRICi19715412. VBIStrPyo79812_0774.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK33818.1.
CP000017 Genomic DNA. Translation: AAZ51322.1.
RefSeqiNP_269097.1. NC_002737.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AEEX-ray1.95A/B1-209[»]
ProteinModelPortaliQ9A076.
SMRiQ9A076.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160490.SPy_0901.

Proteomic databases

PaxDbiQ9A076.
PRIDEiQ9A076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK33818; AAK33818; SPy_0901.
AAZ51322; AAZ51322; M5005_Spy0704.
GeneIDi901056.
KEGGispy:SPy_0901.
spz:M5005_Spy0704.
PATRICi19715412. VBIStrPyo79812_0774.

Phylogenomic databases

eggNOGiENOG41065E1. Bacteria.
COG0461. LUCA.
HOGENOMiHOG000037975.
KOiK00762.
OMAiLQINAIK.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.

Miscellaneous databases

EvolutionaryTraceiQ9A076.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE. 1 hit.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRE_STRP1
AccessioniPrimary (citable) accession number: Q9A076
Secondary accession number(s): Q48Z96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.