Reviewed, UniProtKB/Swiss-Prot Q99ZR0 (KPRS2_STRP1)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 2
      Short name=RPPK 2
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 2
      Short name=P-Rib-PP synthetase 2
      Short name=PRPP synthetase 2

Gene names

Name:	prs2
Ordered Locus Names:	SPy_1123, M5005_Spy0845

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Ribose-phosphate pyrophosphokinase 2 HAMAP MF_00583		PRO_0000141209
Regions
	Region	218 – 231	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q99ZR0-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 43E9795E6E7462A9	FASTA	326	35,708
	10 20 30 40 50 60 MTERYADKQI KLFSLTSNLP IAEKIAKAAG IPLGKMSSRQ FSNGEIMINI EETVRGDDIY 70 80 90 100 110 120 IIQSTSFPVN DNLWELLIMI DACKRASANT VNIVLPYFGY SRQDRVAKPR EPITAKLVAN 130 140 150 160 170 180 MLTKAGIDRV VTLDLHAVQV QGFFDIPVDN LFTVPLFAER YSKLGLSGSD VVVVSPKNSG 190 200 210 220 230 240 IKRARSLAEY LDSPIAIIDY AQDDSEREQG YIIGDVSGKK AILIDDILNT GKTFAEAAKI 250 260 270 280 290 300 LERSGATDTY AVASHGLFAG GAADVLETAP IKEIIVTDSV KTKNRVPENV TYLSASDLIA 310 320 EAIIRIHERR PLSPLFSYQP KGKNNA

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004092 Genomic DNA. Translation: AAK33998.1. CP000017 Genomic DNA. Translation: AAZ51463.1.
RefSeq	NP_269277.1. YP_282208.1.
3D structure databases
HSSP	HSSP built from PDB template 1IBS based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	3572054. 901241.
GenomeReviews	Gene locus SPy_1123 in contig AE004092_GR. Gene locus M5005_Spy0845 in contig CP000017_GR.
KEGG	spy:SPy_1123. spz:M5005_Spy_0845.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q99ZR0.
OMA	NDHLWEL.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY1123-MON. SPYO293653:M5005_SPY0845-MON.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_STRP1

Accession

Primary (citable) accession number: Q99ZR0
Secondary accession number(s): Q48YV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)