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Q99ZM2 (LDHD_STRP1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-lactate dehydrogenase
Short name=D-LDH
EC=1.1.1.28
Alternative name(s):
D-specific D-2-hydroxyacid dehydrogenase
Gene names
Name:ldhD
Synonyms:ddh
Ordered Locus Names:SPy_1170, M5005_Spy0890
OrganismStreptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]
Taxonomic identifier301447 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(R)-lactate + NAD+ = pyruvate + NADH.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionD-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

NAD binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330D-lactate dehydrogenase
PRO_0000075970

Regions

Nucleotide binding155 – 1562NAD By similarity
Nucleotide binding233 – 2353NAD By similarity
Nucleotide binding296 – 2994NAD By similarity

Sites

Active site2351 By similarity
Active site2641 By similarity
Active site2961Proton donor By similarity
Binding site2591NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99ZM2 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 34897A432723B092

FASTA33036,126
        10         20         30         40         50         60 
MKLKLYNVRG EEAVLAKKWA DDNGIEISLT ESPLTPETVK EAEGFDGIAN AQIGPLDDAI 

        70         80         90        100        110        120 
YPLLKEMGIK QIAQHSASVD MYNLDLATEN DIIITNVPSY SPESIAEFTV TIVLNLIRHV 

       130        140        150        160        170        180 
ELIRENVKKQ NFTWGLPIRG RVLGDMTVAI IGTGRIGLAT AKIFKGFGCK VVGYDIYQSD 

       190        200        210        220        230        240 
AAKAVLDYKE SVEEAIKDAD LVSLHMPPTA ENTHLFNSDL FKSFKKGAIL MNMARGAVIE 

       250        260        270        280        290        300 
TQDLLDALDA GLLSGAGIDT YEFEGPYIPK NFEGQEITDS LFKALINHPK VIYTPHAAYY 

       310        320        330 
TDEAVKNLVE GALNATVEII KTGTTTTRVN

« Hide

References

[1]"Complete genome sequence of an M1 strain of Streptococcus pyogenes."
Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. expand/collapse author list , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E.
Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700294 / SF370 / Serotype M1.
[2]"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004092 Genomic DNA. Translation: AAK34037.1.
CP000017 Genomic DNA. Translation: AAZ51508.1.
RefSeqNP_269316.1. NC_002737.1.
YP_282253.1. NC_007297.1.

3D structure databases

ProteinModelPortalQ99ZM2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTRT00000000715; EBSTRP00000000715; EBSTRG00000000715.
EBSTRT00000028763; EBSTRP00000027781; EBSTRG00000028763.
GeneID3572021.
901282.
GenomeReviewsGene locus SPy_1170 in contig AE004092_GR.
Gene locus M5005_Spy0890 in contig CP000017_GR.
KEGGspy:SPy_1170.
spz:M5005_Spy_0890.
PATRIC19715913. VBIStrPyo79812_1021.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000027840.
HOGENOMHBG731446.
OMATWGLPIR.
ProtClustDBPRK08605.

Enzyme and pathway databases

BioCycSPYO160490:SPY1170-MONOMER.
SPYO293653:M5005_SPY0890-MONOMER.

Family and domain databases

InterProIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KOK03778.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. False negative.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLDHD_STRP1
AccessionPrimary (citable) accession number: Q99ZM2
Secondary accession number(s): Q48YR4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2001
Last modified: January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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