Reviewed, UniProtKB/Swiss-Prot Q99ZM2 (LDHD_STRP1)

Names and origin

Protein names

Recommended name:
    D-lactate dehydrogenase
      Short name=D-LDH
    EC=1.1.1.28
Alternative name(s):
    D-specific D-2-hydroxyacid dehydrogenase

Gene names

Name:	ldhD
Synonyms:	ddh
Ordered Locus Names:	SPy_1170, M5005_Spy0890

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	D-lactate dehydrogenase		PRO_0000075970
Regions
	Nucleotide binding	155 – 156	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity
	Binding site	259	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99ZM2-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 34897A432723B092	FASTA	330	36,126
	10 20 30 40 50 60 MKLKLYNVRG EEAVLAKKWA DDNGIEISLT ESPLTPETVK EAEGFDGIAN AQIGPLDDAI 70 80 90 100 110 120 YPLLKEMGIK QIAQHSASVD MYNLDLATEN DIIITNVPSY SPESIAEFTV TIVLNLIRHV 130 140 150 160 170 180 ELIRENVKKQ NFTWGLPIRG RVLGDMTVAI IGTGRIGLAT AKIFKGFGCK VVGYDIYQSD 190 200 210 220 230 240 AAKAVLDYKE SVEEAIKDAD LVSLHMPPTA ENTHLFNSDL FKSFKKGAIL MNMARGAVIE 250 260 270 280 290 300 TQDLLDALDA GLLSGAGIDT YEFEGPYIPK NFEGQEITDS LFKALINHPK VIYTPHAAYY 310 320 330 TDEAVKNLVE GALNATVEII KTGTTTTRVN

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004092 Genomic DNA. Translation: AAK34037.1. CP000017 Genomic DNA. Translation: AAZ51508.1.
RefSeq	NP_269316.1. YP_282253.1.
3D structure databases
HSSP	HSSP built from PDB template 1DXY based on UniProtKB P17584.
ModBase	Search...
Genome annotation databases
GeneID	3572021. 901282.
GenomeReviews	Gene locus SPy_1170 in contig AE004092_GR. Gene locus M5005_Spy0890 in contig CP000017_GR.
KEGG	spy:SPy_1170. spz:M5005_Spy_0890.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q99ZM2.
OMA	TWGLPIR.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY1170-MON. SPYO293653:M5005_SPY0890-MON.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_STRP1

Accession

Primary (citable) accession number: Q99ZM2
Secondary accession number(s): Q48YR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)