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Protein

Formate--tetrahydrofolate ligase 1

Gene

fhs1

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-1004-MONOMER.
SPYO293653:GHFC-986-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase 1UniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetase 1UniRule annotation
Short name:
FHS 1UniRule annotation
Short name:
FTHFS 1UniRule annotation
Gene namesi
Name:fhs1UniRule annotation
Synonyms:fhs, fhs.1
Ordered Locus Names:SPy_1213, M5005_Spy0927
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000750 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligase 1PRO_0000199389Add
BLAST

Proteomic databases

PaxDbiQ99ZI9.

Interactioni

Protein-protein interaction databases

STRINGi160490.SPy_1213.

Structurei

3D structure databases

ProteinModelPortaliQ99ZI9.
SMRiQ99ZI9. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiEEPAHYT.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99ZI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDIEIAQS VALQPITDIV KKVGIDGDDI ELYGKYKAKL SFEKMKAVEA
60 70 80 90 100
NEPGKLILVT AINPTPAGEG KSTMSIGLAD ALNQMGKKTM LALREPSLGP
110 120 130 140 150
VMGIKGGAAG GGYAQVLPME DINLHFTGDM HAITTANNAL SALIDNHLQQ
160 170 180 190 200
GNDLGIDPRR IIWKRVLDLN DRALRQVIVG LGSPVNGVPR EDGFDITVAS
210 220 230 240 250
EIMAILCLAT DLKDLKKRLA DIVVAYTYDR KPVYVRDLKV EGALTLILKD
260 270 280 290 300
AIKPNLVQTI YGTPALIHGG PFANIAHGCN SVLATSTALR LADYTVTEAG
310 320 330 340 350
FGADLGAEKF LNIKVPNLPK APDAIVIVAT LRALKMHGGV AKSDLAAENC
360 370 380 390 400
EAVRLGFANL KRHVENMRQF KVPVVVAINE FVADTEAEIA TLKALCEEIK
410 420 430 440 450
VPVELASVWA NGAEGGLALA KTVVRVIDQE AADYKRLYSD EDTLEEKVIN
460 470 480 490 500
IVTQIYGGKA VQFGPKAKTQ LKQFAEFGWD KLPVCMAKTQ YSFSDNPSLL
510 520 530 540 550
GAPTDFDITI REFVPKTGAG FIVGLTGDVM TMPGLPKVPA AMAMDVAENG

TALGLF
Length:556
Mass (Da):59,531
Last modified:June 1, 2001 - v1
Checksum:i90C6D132DF20EE3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK34072.1.
CP000017 Genomic DNA. Translation: AAZ51545.1.
RefSeqiNP_269351.1. NC_002737.1.
YP_282290.1. NC_007297.1.

Genome annotation databases

EnsemblBacteriaiAAK34072; AAK34072; SPy_1213.
AAZ51545; AAZ51545; M5005_Spy0927.
GeneIDi3571980.
901317.
KEGGispy:SPy_1213.
PATRICi19715989. VBIStrPyo79812_1059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK34072.1.
CP000017 Genomic DNA. Translation: AAZ51545.1.
RefSeqiNP_269351.1. NC_002737.1.
YP_282290.1. NC_007297.1.

3D structure databases

ProteinModelPortaliQ99ZI9.
SMRiQ99ZI9. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160490.SPy_1213.

Proteomic databases

PaxDbiQ99ZI9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK34072; AAK34072; SPy_1213.
AAZ51545; AAZ51545; M5005_Spy0927.
GeneIDi3571980.
901317.
KEGGispy:SPy_1213.
PATRICi19715989. VBIStrPyo79812_1059.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiEEPAHYT.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSPYO160490:GJ81-1004-MONOMER.
SPYO293653:GHFC-986-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700294 / SF370 / Serotype M1.
  2. "Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
    Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
    J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Entry informationi

Entry nameiFTHS1_STRP1
AccessioniPrimary (citable) accession number: Q99ZI9
Secondary accession number(s): Q48YM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.