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Q99YW3

- SYI_STRP1

UniProt

Q99YW3 - SYI_STRP1

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Protein

Isoleucine--tRNA ligase

Gene
ileS, SPy_1513, M5005_Spy1243
Organism
Streptococcus pyogenes serotype M1
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylate By similarity
Binding sitei598 – 5981ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-1246-MONOMER.
SPYO293653:GHFC-1302-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SPy_1513, M5005_Spy1243
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000750: Chromosome, UP000002702: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Isoleucine--tRNA ligaseUniRule annotationPRO_0000098481Add
BLAST

Proteomic databases

PaxDbiQ99YW3.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi160490.SPy_1513.

Structurei

3D structure databases

ProteinModelPortaliQ99YW3.
SMRiQ99YW3. Positions 1-918.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99YW3-1 [UniParc]FASTAAdd to Basket

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MKLKETLNLG KTAFPMRAGL PNKEPQWQAA WEQAELYKKR QELNAGKPAF    50
HLHDGPPYAN GNIHVGHALN KISKDIIVRS KSMSGFQAPY VPGWDTHGLP 100
IEQVLAKQGI KRKEMDLAEY LEMCRQYALS QVDKQRDDFK RLGVSADWEN 150
PYVTLDPQFE ADQIRVFGAM AEKGYIYRGA KPVYWSWSSE SALAEAEIEY 200
HDIDSTSLYY ANKVKDGKGI LDTNTYIVVW TTTPFTVTAS RGLTVGPDMD 250
YLVVKPAGSD RQYVVAEGLL DSLAGKFGWE SFETLASHKG ADLEYIVTEH 300
PWDTDVEELV ILGDHVTLES GTGIVHTAPG FGEDDYNVGT KYKLEVAVTV 350
DERGLMMENA GPDFHGQFYN KVTPIVIDKL GDLLLAQEVI NHSYPFDWRT 400
KKPIIWRAVP QWFASVSDFR QDILDEIEKT TFHPSWGETR LYNMIRDRGD 450
WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVADLFQEN GSIIWWQKEA 500
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSWNGVMN TKENLSYPAD 550
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AILSQGFVLD GKGEKMSKSK 600
GNIISPNDVA KQYGADILRL WVASVDTDND VRVSMEILGQ VSETYRKIRN 650
TLRFLIANTS DFNPATDTVA YADLGTVDKY MTIVFNQLVA TITDAYERYD 700
FMAIYKAVVN FVTVDLSAFY LDFAKDVVYI EAANSLERRR MQTVFYDILV 750
KITKLLTPIL PHTTEEIWSY LEHESEAFVQ LAEMPVAETF SAQEDILEAW 800
SAFMTLRTQA QKALEEARNA KIIGKSLEAH LTIYASEEVK TLLTALDSDI 850
ALLLIVSQLT IADLADAPAD AVAFEGVAFI VEHAIGEVCE RSRRIDPTTR 900
MRSYNAFVCD HSAKIIEENF PEAVAEGFEE SGK 933
Length:933
Mass (Da):105,039
Last modified:June 1, 2001 - v1
Checksum:i652D104FD2AF2C5F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti773 – 7731H → Y in AAZ51861. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004092 Genomic DNA. Translation: AAK34309.1.
CP000017 Genomic DNA. Translation: AAZ51861.1.
RefSeqiNP_269588.1. NC_002737.1.
YP_282606.1. NC_007297.1.

Genome annotation databases

EnsemblBacteriaiAAK34309; AAK34309; SPy_1513.
AAZ51861; AAZ51861; M5005_Spy1243.
GeneIDi3571672.
901559.
KEGGispy:SPy_1513.
spz:M5005_Spy_1243.
PATRICi19716512. VBIStrPyo79812_1320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004092 Genomic DNA. Translation: AAK34309.1 .
CP000017 Genomic DNA. Translation: AAZ51861.1 .
RefSeqi NP_269588.1. NC_002737.1.
YP_282606.1. NC_007297.1.

3D structure databases

ProteinModelPortali Q99YW3.
SMRi Q99YW3. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160490.SPy_1513.

Chemistry

ChEMBLi CHEMBL2364671.

Proteomic databases

PaxDbi Q99YW3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK34309 ; AAK34309 ; SPy_1513 .
AAZ51861 ; AAZ51861 ; M5005_Spy1243 .
GeneIDi 3571672.
901559.
KEGGi spy:SPy_1513.
spz:M5005_Spy_1243.
PATRICi 19716512. VBIStrPyo79812_1320.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPYO160490:GJ81-1246-MONOMER.
SPYO293653:GHFC-1302-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700294 / SF370 / Serotype M1.
  2. "Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
    Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
    J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Entry informationi

Entry nameiSYI_STRP1
AccessioniPrimary (citable) accession number: Q99YW3
Secondary accession number(s): Q48XR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi