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Q99YW3

- SYI_STRP1

UniProt

Q99YW3 - SYI_STRP1

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-1246-MONOMER.
SPYO293653:GHFC-1302-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SPy_1513, M5005_Spy1243
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000750: Chromosome, UP000002702: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Isoleucine--tRNA ligasePRO_0000098481Add
BLAST

Proteomic databases

PaxDbiQ99YW3.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi160490.SPy_1513.

Structurei

3D structure databases

ProteinModelPortaliQ99YW3.
SMRiQ99YW3. Positions 1-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99YW3-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKLKETLNLG KTAFPMRAGL PNKEPQWQAA WEQAELYKKR QELNAGKPAF
60 70 80 90 100
HLHDGPPYAN GNIHVGHALN KISKDIIVRS KSMSGFQAPY VPGWDTHGLP
110 120 130 140 150
IEQVLAKQGI KRKEMDLAEY LEMCRQYALS QVDKQRDDFK RLGVSADWEN
160 170 180 190 200
PYVTLDPQFE ADQIRVFGAM AEKGYIYRGA KPVYWSWSSE SALAEAEIEY
210 220 230 240 250
HDIDSTSLYY ANKVKDGKGI LDTNTYIVVW TTTPFTVTAS RGLTVGPDMD
260 270 280 290 300
YLVVKPAGSD RQYVVAEGLL DSLAGKFGWE SFETLASHKG ADLEYIVTEH
310 320 330 340 350
PWDTDVEELV ILGDHVTLES GTGIVHTAPG FGEDDYNVGT KYKLEVAVTV
360 370 380 390 400
DERGLMMENA GPDFHGQFYN KVTPIVIDKL GDLLLAQEVI NHSYPFDWRT
410 420 430 440 450
KKPIIWRAVP QWFASVSDFR QDILDEIEKT TFHPSWGETR LYNMIRDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVADLFQEN GSIIWWQKEA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSWNGVMN TKENLSYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AILSQGFVLD GKGEKMSKSK
610 620 630 640 650
GNIISPNDVA KQYGADILRL WVASVDTDND VRVSMEILGQ VSETYRKIRN
660 670 680 690 700
TLRFLIANTS DFNPATDTVA YADLGTVDKY MTIVFNQLVA TITDAYERYD
710 720 730 740 750
FMAIYKAVVN FVTVDLSAFY LDFAKDVVYI EAANSLERRR MQTVFYDILV
760 770 780 790 800
KITKLLTPIL PHTTEEIWSY LEHESEAFVQ LAEMPVAETF SAQEDILEAW
810 820 830 840 850
SAFMTLRTQA QKALEEARNA KIIGKSLEAH LTIYASEEVK TLLTALDSDI
860 870 880 890 900
ALLLIVSQLT IADLADAPAD AVAFEGVAFI VEHAIGEVCE RSRRIDPTTR
910 920 930
MRSYNAFVCD HSAKIIEENF PEAVAEGFEE SGK
Length:933
Mass (Da):105,039
Last modified:June 1, 2001 - v1
Checksum:i652D104FD2AF2C5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti773 – 7731H → Y in AAZ51861. (PubMed:16088826)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK34309.1.
CP000017 Genomic DNA. Translation: AAZ51861.1.
RefSeqiNP_269588.1. NC_002737.1.
YP_282606.1. NC_007297.1.

Genome annotation databases

EnsemblBacteriaiAAK34309; AAK34309; SPy_1513.
AAZ51861; AAZ51861; M5005_Spy1243.
GeneIDi3571672.
901559.
KEGGispy:SPy_1513.
spz:M5005_Spy_1243.
PATRICi19716512. VBIStrPyo79812_1320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK34309.1 .
CP000017 Genomic DNA. Translation: AAZ51861.1 .
RefSeqi NP_269588.1. NC_002737.1.
YP_282606.1. NC_007297.1.

3D structure databases

ProteinModelPortali Q99YW3.
SMRi Q99YW3. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160490.SPy_1513.

Chemistry

ChEMBLi CHEMBL2364671.

Proteomic databases

PaxDbi Q99YW3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK34309 ; AAK34309 ; SPy_1513 .
AAZ51861 ; AAZ51861 ; M5005_Spy1243 .
GeneIDi 3571672.
901559.
KEGGi spy:SPy_1513.
spz:M5005_Spy_1243.
PATRICi 19716512. VBIStrPyo79812_1320.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPYO160490:GJ81-1246-MONOMER.
SPYO293653:GHFC-1302-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700294 / SF370 / Serotype M1.
  2. "Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
    Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
    J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Entry informationi

Entry nameiSYI_STRP1
AccessioniPrimary (citable) accession number: Q99YW3
Secondary accession number(s): Q48XR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3