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Q99YU0 (ASNA_STRP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartate--ammonia ligase

EC=6.3.1.1
Alternative name(s):
Asparagine synthetase A
Gene names
Name:asnA
Ordered Locus Names:SPy_1539, M5005_Spy1269
OrganismStreptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]
Taxonomic identifier301447 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP-Rule MF_00555

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP-Rule MF_00555

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00555.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Asparagine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-asparagine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

tRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA ligase activity

Inferred from electronic annotation. Source: InterPro

aspartate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Aspartate--ammonia ligase HAMAP-Rule MF_00555
PRO_0000195893

Sequences

Sequence LengthMass (Da)Tools
Q99YU0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: B95B324CB73382D1

FASTA33037,385
        10         20         30         40         50         60 
MKKSFIHQQE EISFVKNTFT QYLIAKLDVV EVQGPILSRV GDGMQDNLSG TENPVSVNVL 

        70         80         90        100        110        120 
KIPNATFEVV HSLAKWKRHT LARFGFNEGE GLVVNMKALR PDEDSLDQTH SVYVDQWDWE 

       130        140        150        160        170        180 
KVIPDGKRNL AYLKETVETI YKVIRLTELA VEARYDIEAV LPKKITFIHT EELVAKYPDL 

       190        200        210        220        230        240 
TPKERENAIT KEFGAVFLIG IGGVLPDGKP HDGRAPDYDD WTTETENGYH GLNGDILVWN 

       250        260        270        280        290        300 
DQLGSAFELS SMGIRVDEEA LKRQVEMTGD QDRLGFDWHK SLLNGLFPLT IGGGIGQSRM 

       310        320        330 
VMFLLRKQHI GEVQTSVWPQ EVRDSYDNIL 

« Hide

References

[1]"Complete genome sequence of an M1 strain of Streptococcus pyogenes."
Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. expand/collapse author list , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E.
Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700294 / SF370 / Serotype M1.
[2]"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004092 Genomic DNA. Translation: AAK34333.1.
CP000017 Genomic DNA. Translation: AAZ51887.1.
RefSeqNP_269612.1. NC_002737.1.
YP_282632.1. NC_007297.1.

3D structure databases

ProteinModelPortalQ99YU0.
SMRQ99YU0. Positions 4-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160490.SPy_1539.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK34333; AAK34333; SPy_1539.
AAZ51887; AAZ51887; M5005_Spy1269.
GeneID3571620.
901583.
KEGGspy:SPy_1539.
spz:M5005_Spy_1269.
PATRIC19716564. VBIStrPyo79812_1346.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2502.
HOGENOMHOG000284502.
KOK01914.
OMAKLPFHQM.
OrthoDBEOG64V2FB.

Enzyme and pathway databases

BioCycSPYO160490:GJ81-1270-MONOMER.
SPYO293653:GHFC-1328-MONOMER.
UniPathwayUPA00134; UER00194.

Family and domain databases

HAMAPMF_00555. AsnA.
InterProIPR006195. aa-tRNA-synth_II.
IPR004618. AsnA.
[Graphical view]
PfamPF03590. AsnA. 1 hit.
[Graphical view]
PIRSFPIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMsTIGR00669. asnA. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNA_STRP1
AccessionPrimary (citable) accession number: Q99YU0
Secondary accession number(s): Q48XN8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways