Q99YU0 (ASNA_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	SPy_1539, M5005_Spy1269

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP-Rule MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP-Rule MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP-Rule MF_00555		PRO_0000195893

Sequences

	Sequence		Length	Mass (Da)
	Q99YU0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B95B324CB73382D1	FASTA	330	37,385
	10 20 30 40 50 60 MKKSFIHQQE EISFVKNTFT QYLIAKLDVV EVQGPILSRV GDGMQDNLSG TENPVSVNVL 70 80 90 100 110 120 KIPNATFEVV HSLAKWKRHT LARFGFNEGE GLVVNMKALR PDEDSLDQTH SVYVDQWDWE 130 140 150 160 170 180 KVIPDGKRNL AYLKETVETI YKVIRLTELA VEARYDIEAV LPKKITFIHT EELVAKYPDL 190 200 210 220 230 240 TPKERENAIT KEFGAVFLIG IGGVLPDGKP HDGRAPDYDD WTTETENGYH GLNGDILVWN 250 260 270 280 290 300 DQLGSAFELS SMGIRVDEEA LKRQVEMTGD QDRLGFDWHK SLLNGLFPLT IGGGIGQSRM 310 320 330 VMFLLRKQHI GEVQTSVWPQ EVRDSYDNIL

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK34333.1. CP000017 Genomic DNA. Translation: AAZ51887.1.
RefSeq	NP_269612.1. NC_002737.1. YP_282632.1. NC_007297.1.
3D structure databases
ProteinModelPortal	Q99YU0.
SMR	Q99YU0. Positions 4-330.
ModBase	Search...
Protein-protein interaction databases
STRING	160490.SPy_1539.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK34333; AAK34333; SPy_1539. AAZ51887; AAZ51887; M5005_Spy1269.
GeneID	3571620. 901583.
KEGG	spy:SPy_1539. spz:M5005_Spy_1269.
PATRIC	19716564. VBIStrPyo79812_1346.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2502.
HOGENOM	HOG000284502.
KO	K01914.
OMA	QSRICMF.
ProtClustDB	PRK05425.
Enzyme and pathway databases
UniPathway	UPA00134; UER00194.
Family and domain databases
HAMAP	MF_00555. AsnA.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_STRP1

Accession

Primary (citable) accession number: Q99YU0
Secondary accession number(s): Q48XN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program