Reviewed, UniProtKB/Swiss-Prot Q99YT1 (MSRAB_STRP1)

Names and origin

Protein names

Recommended name:
    Peptide methionine sulfoxide reductase msrA/msrB
Including the following 2 domains:
    1- Recommended name:
            Peptide methionine sulfoxide reductase msrA
                Short name=Protein-methionine-S-oxide reductase
              EC=1.8.4.11
        Alternative name(s):
            Peptide-methionine (S)-S-oxide reductase
              Short name=Peptide Met(O) reductase
    2- Recommended name:
            Peptide methionine sulfoxide reductase msrB
              EC=1.8.4.12
        Alternative name(s):
            Peptide-methionine (R)-S-oxide reductase

Gene names

Name:	msrAB
Synonyms:	msrA
Ordered Locus Names:	SPy_1557, M5005_Spy1282

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine By similarity. HAMAP MF_01400
Catalytic activity	Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin. HAMAP MF_01400 Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin.
Sequence similarities	In the N-terminal section; belongs to the msrA Met sulfoxide reductase family. In the C-terminal section; belongs to the msrB Met sulfoxide reductase family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: HAMAP
Molecular function	peptide-methionine (R)-S-oxide reductase activity Inferred from electronic annotation. Source: EC peptide-methionine-(S)-S-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Peptide methionine sulfoxide reductase msrA/msrB HAMAP MF_01400		PRO_0000138521
Regions
	Region	1 – 153	153	Peptide methionine sulfoxide reductase A HAMAP MF_01400
	Region	170 – 293	124	Peptide methionine sulfoxide reductase B HAMAP MF_01400
Sites
	Active site	8	1	By similarity
	Active site	282	1	By similarity
Experimental info
	Sequence conflict	294	1	A → S in AAZ51900. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q99YT1-1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 226E1374F1038ADF	FASTA	309	35,103
	10 20 30 40 50 60 MIYLAGGCFW GVEEYFSQVD GVLDAVSGYA NGRGDTTNYQ LIHQTGHAET VEVAYDANRI 70 80 90 100 110 120 SLKELLLHFF RIIDPTSLNK QGNDRGSQYR TGIYYTDKAD LAIIDEVFKE KAKDYKKKIV 130 140 150 160 170 180 VEKAPLKHFI KAEDYHQDYL KKNPNGYCHI DINQATYPVI DESKYPKPSA TEIKEKLSAD 190 200 210 220 230 240 EYRVTQKNET EKAFSNRYWD SFDAGIYVDV VTGEPLFSSK DKFESGCGWP SFSRPISPDV 250 260 270 280 290 300 VRYKEDKSFN MTRTEVRSRS GNSHLGHVFT DGPKDQGGLR YCINSLSITF IPKADMEAKG YGYLLSSVE

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK34345.1. Different initiation. CP000017 Genomic DNA. Translation: AAZ51900.1. Different initiation.
RefSeq	NP_269624.1. YP_282645.1.
3D structure databases
SMR	Q99YT1. Positions 2-159, 163-308.
ModBase	Search...
Genome annotation databases
GeneID	3571633. 901767.
GenomeReviews	Gene locus SPy_1557 in contig AE004092_GR. Gene locus M5005_Spy1282 in contig CP000017_GR.
KEGG	spy:SPy_1557. spz:M5005_Spy_1282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG715255.
OMA	GIYYTDK.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY1557-MONOMER. SPYO293653:M5005_SPY1282-MONOMER.
Family and domain databases
HAMAP	MF_01400. MsrB. Fused. [Tree] MF_01401. MsrA. Fused. [Tree]
InterPro	IPR002579. Methionine_sulphoxide_MsrB. IPR011057. Mss4-like. IPR002569. Peptide_Met_Sox_Rdtase_MsrA. [Graphical view]
Gene3D	G3DSA:3.30.1060.10. MsrA. 1 hit. G3DSA:2.170.150.20. MsrB. 1 hit.
Pfam	PF01625. PMSR. 1 hit. PF01641. SelR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00401. msrA. 1 hit. TIGR00357. MsrB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MSRAB_STRP1

Accession

Primary (citable) accession number: Q99YT1
Secondary accession number(s): Q48XM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	February 9, 2010
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)