ID   Q99YS3_STRP1            Unreviewed;       242 AA.
AC   Q99YS3; Q48XL7;
DT   01-JUN-2001, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 125.
DE   RecName: Full=pre-crRNA processing endonuclease {ECO:0000256|PIRNR:PIRNR029950};
DE            EC=3.1.-.- {ECO:0000256|PIRNR:PIRNR029950};
GN   OrderedLocusNames=SPy_1566 {ECO:0000313|EMBL:AAK34353.1};
OS   Streptococcus pyogenes serotype M1.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=301447 {ECO:0000313|EMBL:AAK34353.1, ECO:0000313|Proteomes:UP000000750};
RN   [1] {ECO:0000313|EMBL:AAK34353.1, ECO:0000313|Proteomes:UP000000750}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700294 / SF370 / Serotype M1
RC   {ECO:0000313|Proteomes:UP000000750};
RX   PubMed=11296296; DOI=10.1073/pnas.071559398;
RA   Ferretti J.J., McShan W.M., Adjic D., Savic D., Savic G., Lyon K.,
RA   Primeaux C., Sezate S.S., Surorov A.N., Kenton S., Lai H., Lin S., Qian Y.,
RA   Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L., White J., Yuan X.,
RA   Clifton S.W., Roe B.A., McLaughlin R.E.;
RT   "Complete genome sequence of an M1 strain of Streptococcus pyogenes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001).
RN   [2] {ECO:0007829|PDB:3VZH}
RP   X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
RX   PubMed=23500492; DOI=10.1016/j.jmb.2013.02.032;
RA   Koo Y., Ka D., Kim E.J., Suh N., Bae E.;
RT   "Conservation and variability in the structure and function of the Cas5d
RT   endoribonuclease in the CRISPR-mediated microbial immune system.";
RL   J. Mol. Biol. 425:3799-3810(2013).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       {ECO:0000256|PIRNR:PIRNR029950}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas5 family.
CC       Subtype I-C/Dvulg subfamily. {ECO:0000256|PIRNR:PIRNR029950}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004092; AAK34353.1; -; Genomic_DNA.
DR   RefSeq; NP_269632.1; NC_002737.2.
DR   PDB; 3VZH; X-ray; 1.70 A; A=1-242.
DR   PDBsum; 3VZH; -.
DR   AlphaFoldDB; Q99YS3; -.
DR   SMR; Q99YS3; -.
DR   PaxDb; 1314-HKU360_01331; -.
DR   KEGG; spy:SPy_1566; -.
DR   PATRIC; fig|160490.10.peg.1368; -.
DR   HOGENOM; CLU_093736_0_0_9; -.
DR   OMA; HGFDYPD; -.
DR   Proteomes; UP000000750; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09752; Cas5_I-C; 1.
DR   Gene3D; 3.30.70.2660; -; 1.
DR   InterPro; IPR021124; CRISPR-assoc_prot_Cas5.
DR   InterPro; IPR013422; CRISPR-assoc_prot_Cas5_N.
DR   InterPro; IPR010155; CRISPR-assoc_prot_Cas5d.
DR   NCBIfam; TIGR01876; cas_Cas5d; 1.
DR   NCBIfam; TIGR02593; CRISPR_cas5; 1.
DR   Pfam; PF09704; Cas_Cas5d; 1.
DR   PIRSF; PIRSF029950; Cas_CT1134; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:3VZH};
KW   Antiviral defense {ECO:0000256|ARBA:ARBA00023118,
KW   ECO:0000256|PIRNR:PIRNR029950};
KW   Endonuclease {ECO:0000256|PIRNR:PIRNR029950};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR029950};
KW   Nuclease {ECO:0000256|PIRNR:PIRNR029950};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000750};
KW   RNA-binding {ECO:0000256|PIRNR:PIRNR029950}.
SQ   SEQUENCE   242 AA;  27728 MW;  55E499C60DB06098 CRC64;
     MYRSRDFYVR VSGQRALFTN PATKGGSERS SYSVPTRQAL NGIVDAIYYK PTFTNIVTEV
     KVINQIQTEL QGVRALLHDY SADLSYVSYL SDVVYLIKFH FVWNEDRKDL NSDRLPAKHE
     AIMERSIRKG GRRDVFLGTR ECLGLVDDIS QEEYETTVSY YNGVNIDLGI MFHSFAYPKD
     KKTPLKSYFT KTVMKNGVIT FKAQSECDIV NTLSSYAFKA PEEIKSVNDE CMEYDAMEKG
     EN
//