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Protein

Glutamate 5-kinase

Gene

proB

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATPUniRule annotation1
Binding sitei55SubstrateUniRule annotation1
Binding sitei142SubstrateUniRule annotation1
Binding sitei158Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 179ATPUniRule annotation2
Nucleotide bindingi220 – 226ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:SPy_1672, M5005_Spy1371
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097341 – 273Glutamate 5-kinaseAdd BLAST273

Proteomic databases

PaxDbiQ99YJ7
PRIDEiQ99YJ7

Interactioni

Protein-protein interaction databases

STRINGi160490.SPy_1672

Structurei

3D structure databases

ProteinModelPortaliQ99YJ7
SMRiQ99YJ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiNMINCYE

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q99YJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKRQFEDVT RIVIKIGTSS LVLPTGKINL EKIDQLAFVI SSLMNKGKEV
60 70 80 90 100
ILVSSGAMGF GLDILKMEKR PTNLAKQQAV SSVGQVAMMS LYSQIFAYYQ
110 120 130 140 150
TNVSQILLTR DVVVFPESLA NVTNAFESLI SLGIVPIVNE NDAVSVDEMD
160 170 180 190 200
HATKFGDNDR LSAVVAGITK ADLLIMLSDI DGLFDKNPTI YEDAQLRSHV
210 220 230 240 250
ANITQEIIAS AGGAGSKFGT GGMLSKVQSA QMVFENKGQM VLMNGANPRD
260 270
ILRVLEGQPL GTWFKQIEEV RHD
Length:273
Mass (Da):29,756
Last modified:June 1, 2001 - v1
Checksum:i6986C6CB586DB5A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA Translation: AAK34430.1
CP000017 Genomic DNA Translation: AAZ51989.1
RefSeqiNP_269709.1, NC_002737.2

Genome annotation databases

EnsemblBacteriaiAAK34430; AAK34430; SPy_1672
AAZ51989; AAZ51989; M5005_Spy1371
GeneIDi901919
KEGGispy:SPy_1672
spz:M5005_Spy1371
PATRICifig|160490.10.peg.1455

Similar proteinsi

Entry informationi

Entry nameiPROB_STRP1
AccessioniPrimary (citable) accession number: Q99YJ7
Secondary accession number(s): Q48XD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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