Reviewed, UniProtKB/Swiss-Prot Q99YI8 (GLPO_STRP1)

Names and origin

Protein names

Recommended name:
    Alpha-glycerophosphate oxidase
    EC=1.1.3.21
Alternative name(s):
    Glycerol-3-phosphate oxidase

Gene names

Name:	glpO
Ordered Locus Names:	SPy_1683, M5005_Spy1380

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Caution	As S.pyogenes is unable to produce acid from glycerol, the significance and/or function of the glpO gene in this organism is at present unknown.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Alpha-glycerophosphate oxidase		PRO_0000126110
Regions
	Nucleotide binding	21 – 49	29	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q99YI8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: C5016720426C4BB4	FASTA	612	67,640
	10 20 30 40 50 60 MEFSRETRRL ALQKMQERDL DLLIIGGGIT GAGVALQAAA SGLDTGLIEM QDFAQGTSSR 70 80 90 100 110 120 STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKP DPMLLPVYDE PGSTFSMFRL 130 140 150 160 170 180 KVAMDLYDLL AGVSNTPAAN KVLTKEEVLK REPDLKQEGL LGGGVYLDFR NNDARLVIEN 190 200 210 220 230 240 IKRANRDGAL IASHVKAEDF LLDDNGKIIG VKARDLLSDQ EIIIKAKLVI NTTGPWSDEI 250 260 270 280 290 300 RQFSHKGQPI HQMRPTKGVH LVVDRQKLPV SQPVYVDTGL NDGRMVFVLP REEKTYFGTT 310 320 330 340 350 360 DTDYTGDLEH PQVTQEDVDY LLGVVNNRFP NANVTIDDIE SSWAGLRPLL SGNSASDYNG 370 380 390 400 410 420 GNSGKVSDDS FDHLVDTVKA YINHEDSREA VEKAIKQVET STSEKELDPS AVSRGSSFER 430 440 450 460 470 480 DENGLFTLAG GKITDYRKMA EGALTGIIQI LKEEFGKSFK LINSKTYPVS GGEINPANVD 490 500 510 520 530 540 SEIEAYAQLG TLSGLSMDDA RYLANLYGSN APKVFALTRQ LTAAEGLSLA ETLSLHYAMD 550 560 570 580 590 600 YEMALKPTDY FLRRTNHLLF MRDSLDALID PVINEMAKHF EWSDQERVAQ EDDLRRVIAD 610 NDLSALKGHQ EG

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004092 Genomic DNA. Translation: AAK34439.1. CP000017 Genomic DNA. Translation: AAZ51998.1.
RefSeq	NP_269718.1. YP_282743.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3571534. 901929.
GenomeReviews	Gene locus SPy_1683 in contig AE004092_GR. Gene locus M5005_Spy1380 in contig CP000017_GR.
KEGG	spy:SPy_1683. spz:M5005_Spy_1380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q99YI8.
OMA	Q99YI8. ASRDGQG.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY1683-MON. SPYO293653:M5005_SPY1380-MON.
Family and domain databases
InterPro	IPR000447. FAD-dep_Gly3P_DH. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPO_STRP1

Accession

Primary (citable) accession number: Q99YI8
Secondary accession number(s): Q48XC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)