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Q99Y18 (LACG_STRP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phospho-beta-galactosidase

EC=3.2.1.85
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG
Gene names
Name:lacG
Ordered Locus Names:SPy_1916, M5005_Spy1632
OrganismStreptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]
Taxonomic identifier301447 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP-Rule MF_01574

Pathway

Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP-Rule MF_01574

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence AAZ52250.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlactose catabolic process via tagatose-6-phosphate

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phospho-beta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4684686-phospho-beta-galactosidase HAMAP-Rule MF_01574
PRO_0000260734

Sites

Active site1601Proton donor By similarity
Active site3751Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99Y18 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: C9EEDECAE68BA3F4

FASTA46853,788
        10         20         30         40         50         60 
MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYNRYPVDL 

        70         80         90        100        110        120 
KLSEEFGVNG IRISIAWSRI FPTGKGEVNP KGVEYYHNLF AECHKRHVEP FVTLHHFDTP 

       130        140        150        160        170        180 
EALHSDGDFL NRENIEHFVN YAEFCFKEFS EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 

       190        200        210        220        230        240 
DLAKVFQSHH NMMVSHARAV KLFKDSGYSG EIGVVHALPT KYPFDANNPD DVRAAELEDI 

       250        260        270        280        290        300 
IHNKFILDAT YLGKYSDKTM EGVNHILEVN GGELDLREED FAALDAAKDL NDFLGINYYM 

       310        320        330        340        350        360 
SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRKAPVDV PKTDWDWIIF PQGLYDQIMR 

       370        380        390        400        410        420 
VKADYPNYKK IYITENGLGY KDEFVDNTVY DGGRIDYVKK HLEVISDAIS DGANVKGYFM 

       430        440        450        460 
WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAYWYKK VAETQVIE 

« Hide

References

[1]"Complete genome sequence of an M1 strain of Streptococcus pyogenes."
Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. expand/collapse author list , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E.
Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700294 / SF370 / Serotype M1.
[2]"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004092 Genomic DNA. Translation: AAK34620.1.
CP000017 Genomic DNA. Translation: AAZ52250.1. Different initiation.
RefSeqNP_269899.1. NC_002737.1.
YP_282995.2. NC_007297.1.

3D structure databases

ProteinModelPortalQ99Y18.
SMRQ99Y18. Positions 1-468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160490.SPy_1916.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbQ99Y18.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK34620; AAK34620; SPy_1916.
AAZ52250; AAZ52250; M5005_Spy1632.
GeneID3571264.
901600.
KEGGspy:SPy_1916.
spz:M5005_Spy_1632.
PATRIC19717242. VBIStrPyo79812_1665.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088632.
KOK01220.
OMAIGRREAP.
OrthoDBEOG60658J.

Enzyme and pathway databases

BioCycSPYO160490:GJ81-1581-MONOMER.
SPYO293653:GHFC-1711-MONOMER.
UniPathwayUPA00542; UER00605.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
HAMAPMF_01574. LacG.
InterProIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01233. lacG. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLACG_STRP1
AccessionPrimary (citable) accession number: Q99Y18
Secondary accession number(s): Q48WM5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries