Q99XX8 (Q99XX8_STRP1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Putative pullulanase EMBL AAK34661.1

Gene names

Name:	pulA EMBL AAK34661.1
Ordered Locus Names:	SPy_1972

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1165 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Cellular component	Cell wall RuleBase RU000373 Secreted
PTM	Peptidoglycan-anchor RuleBase RU000373
Technical term	3D-structure PDB 2J43 Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cell surface Inferred from electronic annotation. Source: InterPro cell wall Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q99XX8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7A293706D24F2010	FASTA	1,165	128,866
	10 20 30 40 50 60 MKKKVNQGSK RYQYLLKKWG IGFVIAATGT VVLGCTPSIL THQVAAKTIV GLARDEAQQG 70 80 90 100 110 120 DGNAKSGDGL QSSSKEAKPV LDSSSANPAS IAEHHLRMHF KTLPAGESLG SLGLWVWGDV 130 140 150 160 170 180 DQPSKDWPNG AITMTKAKKD DYGYYLDVPL AAKHRQQVSY LINNKAGENL SKDQHISLLT 190 200 210 220 230 240 PKMNEVWIDE NYHAHAYRPL KKGYLRINYH NQSGHYDNLA VWTFKDVKTP TTDWPNGLDL 250 260 270 280 290 300 SHKGHYGAYV DVPLKEGANE IGFLILDKSK TGDAIKVQPK DYLFKELDNH TQVFVKDTDP 310 320 330 340 350 360 KVYNNPYYID QVSLKGAEQT TPNEIKAIFT TLDGLDEDAV KQNIKITDKA GKTVAIDELT 370 380 390 400 410 420 LDRDKSVMTL KGDFKAQGAV YTVTFGEVSQ VARQSWQLKD KLYAYDGELG ATLAKDGSVD 430 440 450 460 470 480 LALWSPSADT VKVVVYDKQD QTRVVGQADL TKSDKGVWRA HLTSDSVKGI SDYTGYYYLY 490 500 510 520 530 540 EITRGQEKVM VLDPYAKSLA AWNDATATDD IKTAKAAFID PSKLGPTGLD FAKINNFKKR 550 560 570 580 590 600 EDAIIYEAHV RDFTSDKALE GKLTHPFGTF SAFVEQLDYL KDLGVTHVQL LPVLSYFYAN 610 620 630 640 650 660 ELDKSRSTAY TSSDNNYNWG YDPQHYFALS GMYSANPNDP ALRIAELKNL VNEIHKRGMG 670 680 690 700 710 720 VIFDVVYNHT ARTYLFEDLE PNYYHFMNAD GTARESFGGG RLGTTHAMSR RILVDSITYL 730 740 750 760 770 780 TREFKVDGFR FDMMGDHDAA AIEQAFKAAK AINPNTIMIG EGWRTYQGDE GKKEIAADQD 790 800 810 820 830 840 WMKATNTVGV FSDDIRNTLK SGFPNEGTAA FITGGAKNLE GLFKTIKAQP GNFEADAPGD 850 860 870 880 890 900 VVQYIAAHDN LTLHDVIAKS INKDPKVAEE EIHKRIRLGN TMILTAQGTA FIHSGQEYGR 910 920 930 940 950 960 TKQLLNPDYK TKASDDKVPN KATLIDAVAQ YPYFIHDSYD SSDAVNHFDW AKATDSIAHP 970 980 990 1000 1010 1020 ISNQTKAYTQ GLIALRRSTD AFTKATKAEV DRDVTLITQA GQDGIQQEDL IMGYQTVASN 1030 1040 1050 1060 1070 1080 GDRYAVFVNA DNKTRKVVLP QAYRYLLGAQ VLVDAEQAGV TAIAKPKGVQ FTKEGLTIEG 1090 1100 1110 1120 1130 1140 LTALVLKVSS KTANPSQQKS QTDNHQTKTP DGSKDLDKSL MTRPKRAKTN QKLPKTGEAS 1150 1160 SKGLLAAGIA LLLLAISLLM KRQKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.
[2]	"Identification and structural basis of binding to host lung glycogen by streptococcal virulence factors." van Bueren A.L., Higgins M., Wang D., Burke R.D., Boraston A.B. Nat. Struct. Mol. Biol. 14:76-84(2007) [PubMed: 17187076] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 94-310.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE004092 Genomic DNA. Translation: AAK34661.1.

RefSeq

NP_269940.1. NC_002737.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J43	X-ray	1.60	A/B	94-310	[»]

ProteinModelPortal

Q99XX8.

SMR

Q99XX8. Positions 92-310.

ModBase

Search...

Protein family/group databases

CAZy

CBM41. Carbohydrate-Binding Module Family 41.
CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBSTRT00000001387; EBSTRP00000001387; EBSTRG00000001387.

GeneID

901644.

GenomeReviews

Gene locus SPy_1972 in contig AE004092_GR.

KEGG

spy:SPy_1972.

PATRIC

19717339. VBIStrPyo79812_1715.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000026660.

HOGENOM

HBG575037.

OMA

KSGFPNE.

ProtClustDB

CLSK797669.

Enzyme and pathway databases

BioCyc

SPYO160490:SPY1972-MONOMER.

Family and domain databases

InterPro

IPR015902. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
IPR019948. Gram-positive_anchor.
IPR013783. Ig-like_fold.
IPR019931. LPXTG-motif_cell_wall_anchor.
IPR005323. PUD.
IPR011838. Pullulan_Gpos.
IPR001899. Surface_protein_Gram_pos_cocci.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.

Pfam

PF00128. Alpha-amylase. 2 hits.
PF02922. CBM_48. 1 hit.
PF00746. Gram_pos_anchor. 1 hit.
PF03714. PUD. 2 hits.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

TIGRFAMs

TIGR01167. LPXTG_anchor. 1 hit.
TIGR02102. Pullulan_Gpos. 1 hit.

PROSITE

PS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q99XX8_STRP1

Accession

Primary (citable) accession number: Q99XX8

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)