Q99XR2 (FTHS2_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase 2
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase 2
Short name=FHS 2
Short name=FTHFS 2

Gene names

Name:	fhs2
Synonyms:	fhs.2
Ordered Locus Names:	SPy_2085, M5005_Spy1774

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Formate--tetrahydrofolate ligase 2 HAMAP MF_01543		PRO_0000199390
Regions
	Nucleotide binding	66 – 73	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99XR2 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CB07C9FCE90B34E7	FASTA	557	59,054
	10 20 30 40 50 60 MVLSDIEIAN SVTMEPISKV ADQLGIDKEA LCLYGKYKAK IDARQLVALK NKPDGKLILV 70 80 90 100 110 120 TAISPTPAGE GKTTTSVGLV DALSAIGKKA VIALREPSLG PVFGVKGGAA GGGHAQVVPM 130 140 150 160 170 180 EDINLHFTGD FHAIGVANNL LAALIDNHIH HGNSLGIDSR RITWKRVVDM NDRQLRHIVD 190 200 210 220 230 240 GLQGKVNGIP REDGYDITVA SEIMAILCLS ENISDLKARL EKIIIGYNYQ GEPVTAKDLK 250 260 270 280 290 300 AGGALAALLK DAIHPNLVQT LEHTPALIHG GPFANIAHGC NSVLATKLAL KYGDYAVTEA 310 320 330 340 350 360 GFGADLGAEK FIDIKCRMSG LRPAAVVLVA TIRALKMHGG VPKADLATEN VQAVVDGLPN 370 380 390 400 410 420 LDKHLANIQD VYGLPVVVAI NKFPLDTDAE LQAVYDACDK RGVDVVISDV WANGGAGGRE 430 440 450 460 470 480 LAEKVVTLAE QDNQFRFVYE EDDSIETKLT KIVTKVYGGK GINLSSAAKR ELADLERLGF 490 500 510 520 530 540 GNYPICMAKT QYSFSDDAKK LGAPTDFTVT ISNLKVSAGA GFIVALTGAI MTMPGLPKVP 550 ASETIDIDEE GNITGLF

References

[1]	"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.
[2]	"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK34738.1. CP000017 Genomic DNA. Translation: AAZ52392.1.
RefSeq	NP_270017.1. NC_002737.1. YP_283137.1. NC_007297.1.
3D structure databases
ProteinModelPortal	Q99XR2.
SMR	Q99XR2. Positions 5-555.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000000998; EBSTRP00000000998; EBSTRG00000000998. EBSTRT00000028183; EBSTRP00000027201; EBSTRG00000028183.
GeneID	3571100. 901726.
GenomeReviews	Gene locus SPy_2085 in contig AE004092_GR. Gene locus M5005_Spy1774 in contig CP000017_GR.
KEGG	spy:SPy_2085. spz:M5005_Spy_1774.
PATRIC	19717527. VBIStrPyo79812_1807.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027107.
HOGENOM	HBG677721.
OMA	TDFTVTI.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY2085-MONOMER. SPYO293653:M5005_SPY1774-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS2_STRP1

Accession

Primary (citable) accession number: Q99XR2
Secondary accession number(s): Q48W83

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program