Q99XL5 (SYR_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	SPy_2151, M5005_Spy1808

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00123
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAZ52426.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 563	563	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_0000151620
Regions
	Motif	121 – 131	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q99XL5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: E0F2CAC28D03B613	FASTA	563	63,121
	10 20 30 40 50 60 MDTKTLIASE IAKVVPELEQ DAIFNLLETP KNSDMGDLAF PAFSLAKVLR KAPQMIASEL 70 80 90 100 110 120 AEQIDESQFE KVVAVGPYIN FFLDKAKISS QVLEQVITAG SDYAQQDEGQ GRNVAIDMSS 130 140 150 160 170 180 PNIAKPFSIG HLRSTVIGDS LAHIFAKMGY KPVKINHLGD WGKQFGMLIV AYKKWGDEAA 190 200 210 220 230 240 VQAHPIDELL KLYVRINAEA ETDPTVDEEA REWFRKLEDG DKEATELWQW FRDESLLEFN 250 260 270 280 290 300 RLYDQLHVTF DSYNGEAFYN DKMDEVLDLL EAKNLLVESK GAQVVNLEKY GIEHPALIKK 310 320 330 340 350 360 SDGATLYITR DLAAALYRKR TYDFAKSVYV VGNEQAAHFK QLKAVLKEMG YDWSDDMTHV 370 380 390 400 410 420 AFGLVTKGGA KLSTRKGNVI LLEPTVAEAI NRAASQIEAK NPNLADKEAV AHAVGVGAIK 430 440 450 460 470 480 FYDLKTDRMN GYDFDLEAMV SFEGETGPYV QYAHARIQSI LRKADFTPSA TTTYSLADAE 490 500 510 520 530 540 SWEIIKLIQD FPRIIKRTSD NFEPSIMAKF AINLAQSFNK YYAHTRILDD NSERDNRLAL 550 560 CYATATVLKE ALRLLGVDAP NEM

References

[1]

"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.

[2]

"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK34788.1. CP000017 Genomic DNA. Translation: AAZ52426.1. Different initiation.
RefSeq	NP_270067.1. NC_002737.1. YP_283171.2. NC_007297.1.
3D structure databases
ProteinModelPortal	Q99XL5.
ModBase	Search...
Protein-protein interaction databases
STRING	160490.SPy_2151.
Proteomic databases
PaxDb	Q99XL5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK34788; AAK34788; SPy_2151. AAZ52426; AAZ52426; M5005_Spy1808.
GeneID	3571095. 901785.
KEGG	spy:SPy_2151. spz:M5005_Spy_1808.
PATRIC	19717639. VBIStrPyo79812_1863.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247211.
KO	K01887.
OMA	CKSMLAW.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	SPYO160490:GJ81-1754-MONOMER. SPYO293653:GHFC-1889-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_STRP1

Accession

Primary (citable) accession number: Q99XL5
Secondary accession number(s): Q48W49

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program