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Q99XH4 (SYW_STRP1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Synonyms:trsA
Ordered Locus Names:SPy_2207, M5005_Spy1858
OrganismStreptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]
Taxonomic identifier301447 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136692

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140
Motif202 – 2065"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99XH4 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 3FAFE2E1F4312143

FASTA34038,330
        10         20         30         40         50         60 
MTKPIILTGD RPTGKLHLGH YVGSLKNRVF LQNENKYKMF VFLADQQALT DHAKESELIQ 

        70         80         90        100        110        120 
ESIGNVALDY LSVGLDPKQS TIFIQSQIPE LAELSMYYMN LVSLARLERN PTVKTEIAQK 

       130        140        150        160        170        180 
GFGESIPSGF LVYPVSQAAD ITAFKANLVP VGNDQKPMIE QTREIVRSFN HTYHTDCLVE 

       190        200        210        220        230        240 
PEGIYPENEK AGRLPGLDGN AKMSKSLGNG IYLSDDADTV RKKVMSMYTD PNHIKIEDPG 

       250        260        270        280        290        300 
QIEGNMVFHY LDIFARKEDQ ADIEAMKEHY QIGGLGDVKT KRYLLDILER ELAPIRERRL 

       310        320        330        340 
EYAKDMGEVF RMLQEGSQKA RTVAAKTLSE VKSAMGINYF 

« Hide

References

[1]"Complete genome sequence of an M1 strain of Streptococcus pyogenes."
Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. expand/collapse author list , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E.
Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700294 / SF370 / Serotype M1.
[2]"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004092 Genomic DNA. Translation: AAK34835.1.
CP000017 Genomic DNA. Translation: AAZ52476.1.
RefSeqNP_270114.1. NC_002737.1.
YP_283221.1. NC_007297.1.

3D structure databases

ProteinModelPortalQ99XH4.
SMRQ99XH4. Positions 2-339.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160490.SPy_2207.

Proteomic databases

PaxDbQ99XH4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK34835; AAK34835; SPy_2207.
AAZ52476; AAZ52476; M5005_Spy1858.
GeneID3571030.
901899.
KEGGspy:SPy_2207.
spz:M5005_Spy_1858.
PATRIC19717737. VBIStrPyo79812_1912.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059941.
KOK01867.
OMAVFTHSAV.
OrthoDBEOG686NJQ.

Enzyme and pathway databases

BioCycSPYO160490:GJ81-1801-MONOMER.
SPYO293653:GHFC-1939-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_STRP1
AccessionPrimary (citable) accession number: Q99XH4
Secondary accession number(s): Q48VZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries