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Q99X26 (HSDR_STAAM) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-1 restriction enzyme R protein

EC=3.1.21.3
Alternative name(s):
Type I restriction enzyme R protein
Gene names
Name:hsdR
Ordered Locus Names:SAV0195
OrganismStaphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]
Taxonomic identifier158878 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length929 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Subunit R is required for both nuclease and ATPase activities, but not for modification By similarity.

Catalytic activity

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Subunit structure

The type I restriction/modification system is composed of three polypeptides R, M and S By similarity.

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases By similarity.

Sequence similarities

Belongs to the HsdR family.

Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Type I site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 929929Type-1 restriction enzyme R protein
PRO_0000077266

Regions

Domain254 – 418165Helicase ATP-binding
Nucleotide binding268 – 2747ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99X26 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 7814981B5A925187

FASTA929109,294
        10         20         30         40         50         60 
MAYQSEYALE NEMMNQLEQL GYERVTIRDN KQLLDNFRTI LNERHADKLE GNPLTDKEFQ 

        70         80         90        100        110        120 
RLLTMIDGKS IFESARILRD KLPLRRDDES EIYLSFLDKK SWCKNKFQVT NQVSVEDTYK 

       130        140        150        160        170        180 
ARYDVTILIN GLPLVQVELK RRGIDINEAF NQVKRYRKQN YTGLFRYIQM FIISNGVETR 

       190        200        210        220        230        240 
YFSNNDSELL KSHMFYWSDK QNNRINTLQS FAESFMRPCQ LAKMISRYMI INETDRILMA 

       250        260        270        280        290        300 
MRPYQVYAVE ALIQQATETG NNGYVWHTTG SGKTLTSFKA SQILSQQDDI KKVIFLVDRK 

       310        320        330        340        350        360 
DLDSQTEEEF NKFAKGAVDK TFNTSQLVRQ LNDKSLPLIV TTIQKMAKAI QGNAHLLEQY 

       370        380        390        400        410        420 
KTNKVVFIID ECHRSQFGDM HRLVKQHFKN AQYFGFTGTP RFPENSSQDG RTTADIFGRC 

       430        440        450        460        470        480 
LHTYLIRDAI HDGNVLGFSV DYINTFKNKA LKAEDNSMVE AIDTEEVWLA DKRVELVTRH 

       490        500        510        520        530        540 
IINNHDKYTR NRQYSSIFTV QSIHALIKYY ETFKRLNKKL EQPLTIAGIF TFKPNEDDRD 

       550        560        570        580        590        600 
GEVPYHSREK LEIMISDYNK KFETNFSTDT TNEYFNHISK NVKKGVKDSK IDILIVVNMF 

       610        620        630        640        650        660 
LTGFDSKVLN TLYVDKNLMY HDLIQAYSRT NRVEKESKPF GKIVNYRDLK KETDDALRVF 

       670        680        690        700        710        720 
SQTNDTDTIL MRSYEEYKKE FMDAYRELKM IVPTPHMVDD IQDEEELKRF VEAYRLLAKI 

       730        740        750        760        770        780 
ILRLKAFDEF EFTIDEIGMD EQENEDYKSK YLAVYDQVKR ATAEKNKVSI LNDIDFEIEM 

       790        800        810        820        830        840 
MRNDTINVNY IMNILRQIDL EDKAEQRRNQ EQIRRILDHA DDPTLRLKRD LIREFIDNVV 

       850        860        870        880        890        900 
PSLNKDDDID QEYVNFESIK KEAEFKGFAG ERSIDEQALK TISNDYQYSG VVNPHHLKKM 

       910        920 
IGDLPLKEKR KARKAIESFV AETTEKYGV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000017 Genomic DNA. Translation: BAB56357.1.
RefSeqNP_370719.1. NC_002758.2.

3D structure databases

ProteinModelPortalQ99X26.
SMRQ99X26. Positions 2-783.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING158878.SAV0195.

Protein family/group databases

REBASE5123. SauMu50ORF195P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB56357; BAB56357; SAV0195.
GeneID1120154.
KEGGsav:SAV0195.
PATRIC19560997. VBIStaAur52173_0196.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0610.
HOGENOMHOG000003518.
KOK01153.
OMANNGYIWH.
OrthoDBEOG6JTCB4.
PhylomeDBQ99X26.

Enzyme and pathway databases

BioCycSAUR158878:GJJ5-195-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
IPR022625. TypeI_RM_Rsu_C.
[Graphical view]
PfamPF12008. EcoR124_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00348. hsdR. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHSDR_STAAM
AccessionPrimary (citable) accession number: Q99X26
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families