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Q99WZ6 (PFLA_STAAM) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate formate-lyase-activating enzyme

Short name=PFL-activating enzyme
EC=1.97.1.4
Gene names
Name:pflA
Ordered Locus Names:SAV0227
OrganismStaphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]
Taxonomic identifier158878 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Pyruvate formate-lyase-activating enzyme
PRO_0000271712

Sites

Metal binding291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding331Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding361Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99WZ6 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 892A603E273F6C89

FASTA25128,499
        10         20         30         40         50         60 
MLKGHLHSVE SLGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKISEPSRE VTVDEMVNEI 

        70         80         90        100        110        120 
LPYKPYFDAS GGGVTVSGGE PLLQMPFLEK LFAELKENGV HTCLDTSAGC ANDTKAFQRH 

       130        140        150        160        170        180 
FEELQKHTDL ILLDIKHIDN DKHIRLTGKP NTHILNFARK LSDMKQPVWI RHVLVPGYSD 

       190        200        210        220        230        240 
DKDDLIKLGE FINSLDNVEK FEILPYHQLG VHKWKTLGIA YELEDVEAPD DEAVKAAYRY 

       250 
VNFKGKIPVE L 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000017 Genomic DNA. Translation: BAB56389.1.
RefSeqNP_370751.1. NC_002758.2.

3D structure databases

ProteinModelPortalQ99WZ6.
SMRQ99WZ6. Positions 3-250.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING158878.SAV0227.

Proteomic databases

PRIDEQ99WZ6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB56389; BAB56389; SAV0227.
GeneID1120185.
KEGGsav:SAV0227.
PATRIC19561064. VBIStaAur52173_0229.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1180.
HOGENOMHOG000011458.
KOK04069.
OMAYVHSVES.
OrthoDBEOG64FKHC.
ProtClustDBCLSK884495.

Enzyme and pathway databases

BioCycSAUR158878:GJJ5-228-MONOMER.

Family and domain databases

InterProIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsTIGR02493. PFLA. 1 hit.
PROSITEPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFLA_STAAM
AccessionPrimary (citable) accession number: Q99WZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: June 1, 2001
Last modified: March 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families