Reviewed, UniProtKB/Swiss-Prot Q99WW8 (ISPD2_STAAM)

Names and origin

Protein names

Recommended name:
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2
    EC=2.7.7.60
Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2
    MEP cytidylyltransferase 2
      Short name=MCT 2

Gene names

Name:	ispD2
Ordered Locus Names:	SAV0255

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the ispD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 HAMAP MF_00108		PRO_0000075616
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99WW8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B19F7EA82D847A0F	FASTA	238	26,657
	10 20 30 40 50 60 MKYAGILAGG IGSRMGNVPL PKQFLDLDNK PILIHTLEKF ILINDFEKII IATPQQWMTH 70 80 90 100 110 120 TKDTLRKFKI SDERIEVIQG GSDRNDTIMN IVKHIESTNG INDDDVIVTH DAVRPFLTHR 130 140 150 160 170 180 IIKENIQAAL EYGAVDTVID AIDTIVTSKD NQTIDAIPVR NEMYQGQTPQ SFNINLLKES 190 200 210 220 230 YAQLSDEQKS ILSDACKIIV ETNKPVRLVK GELYNIKVTT PYDLKVANAI IRGGIADD

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB56417.1.
RefSeq	NP_370779.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q99WW8.
2-D gel databases
World-2DPAGE	0002:Q99WW8.
Genome annotation databases
GeneID	1120213.
GenomeReviews	Gene locus SAV0255 in contig BA000017_GR.
KEGG	sav:SAV0255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	REQQDFW.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV0255-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD2_STAAM

Accession

Primary (citable) accession number: Q99WW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2001
Last modified:	January 19, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)