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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2

Gene

ispD2

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).By similarity

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (ispD2), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (ispD1)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei14 – 141Transition state stabilizerBy similarity
Sitei22 – 221Transition state stabilizerBy similarity
Sitei160 – 1601Positions MEP for the nucleophilic attackBy similarity
Sitei217 – 2171Positions MEP for the nucleophilic attackBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-256-MONOMER.
UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (EC:2.7.7.60)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2
MEP cytidylyltransferase 2
Short name:
MCT 2
Gene namesi
Name:ispD2
Ordered Locus Names:SAV0255
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2382382-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2PRO_0000075616Add
BLAST

Proteomic databases

PaxDbiQ99WW8.

2D gel databases

World-2DPAGE0002:Q99WW8.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV0255.

Structurei

3D structure databases

ProteinModelPortaliQ99WW8.
SMRiQ99WW8. Positions 21-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.Curated

Phylogenomic databases

eggNOGiENOG4108QHG. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiPQVFDAD.
PhylomeDBiQ99WW8.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99WW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYAGILAGG IGSRMGNVPL PKQFLDLDNK PILIHTLEKF ILINDFEKII
60 70 80 90 100
IATPQQWMTH TKDTLRKFKI SDERIEVIQG GSDRNDTIMN IVKHIESTNG
110 120 130 140 150
INDDDVIVTH DAVRPFLTHR IIKENIQAAL EYGAVDTVID AIDTIVTSKD
160 170 180 190 200
NQTIDAIPVR NEMYQGQTPQ SFNINLLKES YAQLSDEQKS ILSDACKIIV
210 220 230
ETNKPVRLVK GELYNIKVTT PYDLKVANAI IRGGIADD
Length:238
Mass (Da):26,657
Last modified:June 1, 2001 - v1
Checksum:iB19F7EA82D847A0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB56417.1.
RefSeqiWP_000872486.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB56417; BAB56417; SAV0255.
KEGGisav:SAV0255.
PATRICi19561124. VBIStaAur52173_0259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB56417.1.
RefSeqiWP_000872486.1. NC_002758.2.

3D structure databases

ProteinModelPortaliQ99WW8.
SMRiQ99WW8. Positions 21-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV0255.

2D gel databases

World-2DPAGE0002:Q99WW8.

Proteomic databases

PaxDbiQ99WW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB56417; BAB56417; SAV0255.
KEGGisav:SAV0255.
PATRICi19561124. VBIStaAur52173_0259.

Phylogenomic databases

eggNOGiENOG4108QHG. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiPQVFDAD.
PhylomeDBiQ99WW8.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.
BioCyciSAUR158878:GJJ5-256-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISPD2_STAAM
AccessioniPrimary (citable) accession number: Q99WW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.