Reviewed, UniProtKB/Swiss-Prot Q99WQ6 (LIP2_STAAM)

Names and origin

Protein names

Recommended name:
    Lipase 2
    EC=3.1.1.3
Alternative name(s):
    Glycerol ester hydrolase 2

Gene names

Name:	lip2
Synonyms:	geh
Ordered Locus Names:	SAV0320

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Propeptide	38 – 296	259	By similarity		PRO_0000045188
	Chain	297 – 691	395	Lipase 2		PRO_0000045189
Sites
	Active site	413	1	Charge relay system By similarity
	Active site	646	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q99WQ6-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 11F2117D26C54C7A	FASTA	691	76,543
	10 20 30 40 50 60 MLRGQEERKY SIRKYSIGVV SVLAATMFVV SSHEAQASEK TPTSNAAAQK ETLNQPGEQG 70 80 90 100 110 120 NAITSHQMQS GKQLDDMHKE NGKSGTVTEG KDTLQSSKHQ STQNSKTIRT QNDNQVKQDS 130 140 150 160 170 180 ERQGSKQSHQ NNATNNTERQ NDQVQNTHHA ERNGSQSTTS QSNDVDKSQP SIPAQKVLPN 190 200 210 220 230 240 HDKAAPTSTT PPSNDKTAPK STKAQDATTD KHPNQQDTHQ PAHQIIDAKQ DDTVRQSEQK 250 260 270 280 290 300 PQVGDLSKHI DGQNSPEKPT DKNTDNKQLI KDALQAPKTR STTNAAADAK KVRPLKANQV 310 320 330 340 350 360 QPLNKYPVVF VHGFLGLVGD NAPALYPNYW GGNKFKVIEE LRKQGYNVHQ ASVSAFGSNY 370 380 390 400 410 420 DRAVELYYYI KGGRVDYGAA HAAKYGHERY GKTYKGIMPN WEPGKKVHLV GHSMGGQTIR 430 440 450 460 470 480 LMEEFLRNGN KEEIAYHKAH GGEISPLFTG GHNNMVASIT TLATPHNGSQ AADKFGNTEA 490 500 510 520 530 540 VRKIMFALNR FMGNKYSNID LGLTQWGFKQ LPNESYIDYI KRVSKSKIWT SDDNAAYDLT 550 560 570 580 590 600 LDGSAKLNNM TSMNPNITYT TYTGVSSHTG PLGYENPDLG TFFLMDTTSR IIGHDAREEW 610 620 630 640 650 660 RKNDGVVPVI SSLHPSNQPF INVTNDEPAT RRGIWQVKPI IQGWDHVDFI GVDFLDFKRK 670 680 690 GAELANFYTG IINDLLRVEA TESKGTQLKA S

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000017 Genomic DNA. Translation: BAB56482.1.
RefSeq	NP_370844.1.
3D structure databases
HSSP	HSSP built from PDB template 1KU0 based on UniProtKB O66015.
ModBase	Search...
Protein-protein interaction databases
STRING	Q99WQ6.
Genome annotation databases
GeneID	1120278.
GenomeReviews	Gene locus SAV0320 in contig BA000017_GR.
KEGG	sav:SAV0320.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q99WQ6.
OMA	ETINRIG.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV0320-MON.
Family and domain databases
InterPro	IPR005877. Gpos_YSIRK. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF04650. YSIRK_signal. 1 hit. [Graphical view]
TIGRFAMs	TIGR01168. YSIRK_signal. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP2_STAAM

Accession

Primary (citable) accession number: Q99WQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)