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Q99W51

- TADA_STAAM

UniProt

Q99W51 - TADA_STAAM

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Protein

tRNA-specific adenosine deaminase

Gene
tadA, SAV0558
Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2) By similarity.UniRule annotation

Catalytic activityi

Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Zinc; via pros nitrogen; catalytic
Active sitei55 – 551Proton donor By similarity
Metal bindingi83 – 831Zinc; catalytic
Metal bindingi86 – 861Zinc; catalytic

GO - Molecular functioni

  1. tRNA-specific adenosine-34 deaminase activity Source: UniProtKB-HAMAP
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA wobble adenosine to inosine editing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-576-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase (EC:3.5.4.33)
Gene namesi
Name:tadA
Ordered Locus Names:SAV0558
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 156156tRNA-specific adenosine deaminaseUniRule annotationPRO_0000423949Add
BLAST

2D gel databases

World-2DPAGE0002:Q99W51.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-29048N.
STRINGi158878.SAV0558.

Structurei

Secondary structure

1
156
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 2019
Beta strandi27 – 326
Beta strandi35 – 417
Helixi44 – 474
Helixi54 – 6613
Beta strandi74 – 807
Helixi84 – 929
Beta strandi96 – 1027
Turni105 – 1073
Beta strandi127 – 1293
Helixi134 – 15017

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B3JX-ray2.00A/B/C/D1-156[»]
ProteinModelPortaliQ99W51.
SMRiQ99W51. Positions 1-152.

Miscellaneous databases

EvolutionaryTraceiQ99W51.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 101101CMP/dCMP deaminase zinc-bindingAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0590.
HOGENOMiHOG000085050.
KOiK11991.
OMAiGWRLEGC.
OrthoDBiEOG64FKGZ.
PhylomeDBiQ99W51.

Family and domain databases

HAMAPiMF_00972. tRNA_aden_deaminase.
InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99W51-1 [UniParc]FASTAAdd to Basket

« Hide

MTNDIYFMTL AIEEAKKAAQ LGEVPIGAII TKDDEVIARA HNLRETLQQP    50
TAHAEHIAIE RAAKVLGSWR LEGCTLYVTL EPCVMCAGTI VMSRIPRVVY 100
GADDPKGGCS GSLMNLLQQS NFNHRAIVDK GVLKEACSTL LTTFFKNLRA 150
NKKSTN 156
Length:156
Mass (Da):17,090
Last modified:June 1, 2001 - v1
Checksum:iAABDDD24AF14DC12
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB56720.1.
PIRiH89823.
RefSeqiNP_371082.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB56720; BAB56720; SAV0558.
GeneIDi1120531.
KEGGisav:SAV0558.
PATRICi19561768. VBIStaAur52173_0564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB56720.1 .
PIRi H89823.
RefSeqi NP_371082.1. NC_002758.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2B3J X-ray 2.00 A/B/C/D 1-156 [» ]
ProteinModelPortali Q99W51.
SMRi Q99W51. Positions 1-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29048N.
STRINGi 158878.SAV0558.

2D gel databases

World-2DPAGE 0002:Q99W51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB56720 ; BAB56720 ; SAV0558 .
GeneIDi 1120531.
KEGGi sav:SAV0558.
PATRICi 19561768. VBIStaAur52173_0564.

Phylogenomic databases

eggNOGi COG0590.
HOGENOMi HOG000085050.
KOi K11991.
OMAi GWRLEGC.
OrthoDBi EOG64FKGZ.
PhylomeDBi Q99W51.

Enzyme and pathway databases

BioCyci SAUR158878:GJJ5-576-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q99W51.

Family and domain databases

HAMAPi MF_00972. tRNA_aden_deaminase.
InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view ]
Pfami PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53927. SSF53927. 1 hit.
PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.
  2. "Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA."
    Losey H.C., Ruthenburg A.J., Verdine G.L.
    Nat. Struct. Mol. Biol. 13:153-159(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ZINC AND RNA, COFACTOR, SUBUNIT.
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiTADA_STAAM
AccessioniPrimary (citable) accession number: Q99W51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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