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Q99W51

- TADA_STAAM

UniProt

Q99W51 - TADA_STAAM

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Protein

tRNA-specific adenosine deaminase

Gene

tadA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).UniRule annotation

Catalytic activityi

Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.UniRule annotation

Cofactori

Zn2+1 PublicationUniRule annotationNote: Binds 1 zinc ion per subunit.1 PublicationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Zinc; via pros nitrogen; catalytic1 PublicationUniRule annotation
Active sitei55 – 551Proton donorUniRule annotation
Metal bindingi83 – 831Zinc; catalytic1 PublicationUniRule annotation
Metal bindingi86 – 861Zinc; catalytic1 PublicationUniRule annotation

GO - Molecular functioni

  1. tRNA-specific adenosine-34 deaminase activity Source: UniProtKB-HAMAP
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA wobble adenosine to inosine editing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-576-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminaseUniRule annotation (EC:3.5.4.33UniRule annotation)
Gene namesi
Name:tadAUniRule annotation
Ordered Locus Names:SAV0558
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 156156tRNA-specific adenosine deaminasePRO_0000423949Add
BLAST

2D gel databases

World-2DPAGE0002:Q99W51.

Interactioni

Subunit structurei

Homodimer.1 PublicationUniRule annotation

Protein-protein interaction databases

DIPiDIP-29048N.
STRINGi158878.SAV0558.

Structurei

Secondary structure

1
156
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 2019Combined sources
Beta strandi27 – 326Combined sources
Beta strandi35 – 417Combined sources
Helixi44 – 474Combined sources
Helixi54 – 6613Combined sources
Beta strandi74 – 807Combined sources
Helixi84 – 929Combined sources
Beta strandi96 – 1027Combined sources
Turni105 – 1073Combined sources
Beta strandi127 – 1293Combined sources
Helixi134 – 15017Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B3JX-ray2.00A/B/C/D1-156[»]
ProteinModelPortaliQ99W51.
SMRiQ99W51. Positions 1-152.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99W51.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 101101CMP/dCMP deaminase zinc-bindingUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation
Contains 1 CMP/dCMP deaminase zinc-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0590.
HOGENOMiHOG000085050.
KOiK11991.
OMAiGWRLEGC.
OrthoDBiEOG64FKGZ.
PhylomeDBiQ99W51.

Family and domain databases

HAMAPiMF_00972. tRNA_aden_deaminase.
InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view]
PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99W51-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNDIYFMTL AIEEAKKAAQ LGEVPIGAII TKDDEVIARA HNLRETLQQP
60 70 80 90 100
TAHAEHIAIE RAAKVLGSWR LEGCTLYVTL EPCVMCAGTI VMSRIPRVVY
110 120 130 140 150
GADDPKGGCS GSLMNLLQQS NFNHRAIVDK GVLKEACSTL LTTFFKNLRA

NKKSTN
Length:156
Mass (Da):17,090
Last modified:June 1, 2001 - v1
Checksum:iAABDDD24AF14DC12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB56720.1.
PIRiH89823.
RefSeqiNP_371082.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB56720; BAB56720; SAV0558.
GeneIDi1120531.
KEGGisav:SAV0558.
PATRICi19561768. VBIStaAur52173_0564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB56720.1 .
PIRi H89823.
RefSeqi NP_371082.1. NC_002758.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2B3J X-ray 2.00 A/B/C/D 1-156 [» ]
ProteinModelPortali Q99W51.
SMRi Q99W51. Positions 1-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29048N.
STRINGi 158878.SAV0558.

2D gel databases

World-2DPAGE 0002:Q99W51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB56720 ; BAB56720 ; SAV0558 .
GeneIDi 1120531.
KEGGi sav:SAV0558.
PATRICi 19561768. VBIStaAur52173_0564.

Phylogenomic databases

eggNOGi COG0590.
HOGENOMi HOG000085050.
KOi K11991.
OMAi GWRLEGC.
OrthoDBi EOG64FKGZ.
PhylomeDBi Q99W51.

Enzyme and pathway databases

BioCyci SAUR158878:GJJ5-576-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q99W51.

Family and domain databases

HAMAPi MF_00972. tRNA_aden_deaminase.
InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view ]
Pfami PF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view ]
SUPFAMi SSF53927. SSF53927. 1 hit.
PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.
  2. "Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA."
    Losey H.C., Ruthenburg A.J., Verdine G.L.
    Nat. Struct. Mol. Biol. 13:153-159(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ZINC AND RNA, COFACTOR, SUBUNIT.
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiTADA_STAAM
AccessioniPrimary (citable) accession number: Q99W51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3