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Q99W51 (TADA_STAAM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-specific adenosine deaminase

EC=3.5.4.33
Gene names
Name:tadA
Ordered Locus Names:SAV0558
OrganismStaphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]
Taxonomic identifier158878 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length156 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2) By similarity. HAMAP-Rule MF_00972

Catalytic activity

Adenine34 in tRNA + H2O = hypoxanthine34 in tRNA + NH3. HAMAP-Rule MF_00972

Cofactor

Binds 1 zinc ion per subunit. Ref.2

Subunit structure

Homodimer. Ref.2

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Contains 1 CMP/dCMP deaminase zinc-binding domain.

Ontologies

Keywords
   Biological processtRNA processing
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processtRNA wobble adenosine to inosine editing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functiontRNA-specific adenosine-34 deaminase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 156156tRNA-specific adenosine deaminase HAMAP-Rule MF_00972
PRO_0000423949

Regions

Domain1 – 101101CMP/dCMP deaminase zinc-binding

Sites

Active site551Proton donor By similarity
Metal binding531Zinc; via pros nitrogen; catalytic
Metal binding831Zinc; catalytic
Metal binding861Zinc; catalytic

Secondary structure

....................... 156
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q99W51 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: AABDDD24AF14DC12

FASTA15617,090
        10         20         30         40         50         60 
MTNDIYFMTL AIEEAKKAAQ LGEVPIGAII TKDDEVIARA HNLRETLQQP TAHAEHIAIE 

        70         80         90        100        110        120 
RAAKVLGSWR LEGCTLYVTL EPCVMCAGTI VMSRIPRVVY GADDPKGGCS GSLMNLLQQS 

       130        140        150 
NFNHRAIVDK GVLKEACSTL LTTFFKNLRA NKKSTN 

« Hide

References

« Hide 'large scale' references
[1]"Whole genome sequencing of meticillin-resistant Staphylococcus aureus."
Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. expand/collapse author list , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K.
Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Mu50 / ATCC 700699.
[2]"Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA."
Losey H.C., Ruthenburg A.J., Verdine G.L.
Nat. Struct. Mol. Biol. 13:153-159(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ZINC AND RNA, COFACTOR, SUBUNIT.
Strain: Mu50 / ATCC 700699.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000017 Genomic DNA. Translation: BAB56720.1.
PIRH89823.
RefSeqNP_371082.1. NC_002758.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2B3JX-ray2.00A/B/C/D1-156[»]
ProteinModelPortalQ99W51.
SMRQ99W51. Positions 1-152.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-29048N.
STRING158878.SAV0558.

2D gel databases

World-2DPAGE0002:Q99W51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB56720; BAB56720; SAV0558.
GeneID1120531.
KEGGsav:SAV0558.
PATRIC19561768. VBIStaAur52173_0564.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0590.
HOGENOMHOG000085050.
KOK11991.
OMAIQARMGK.
OrthoDBEOG64FKGZ.
ProtClustDBCLSK884723.

Enzyme and pathway databases

BioCycSAUR158878:GJJ5-576-MONOMER.

Family and domain databases

HAMAPMF_00972. tRNA_aden_deaminase.
InterProIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view]
PfamPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMSSF53927. SSF53927. 1 hit.
PROSITEPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ99W51.

Entry information

Entry nameTADA_STAAM
AccessionPrimary (citable) accession number: Q99W51
Entry history
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references