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Q99W51

- TADA_STAAM

UniProt

Q99W51 - TADA_STAAM

Protein

tRNA-specific adenosine deaminase

Gene

tadA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).UniRule annotation

    Catalytic activityi

    Adenine(34) in tRNA + H2O = hypoxanthine(34) in tRNA + NH3.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.1 PublicationUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Zinc; via pros nitrogen; catalytic1 PublicationUniRule annotation
    Active sitei55 – 551Proton donorUniRule annotation
    Metal bindingi83 – 831Zinc; catalytic1 PublicationUniRule annotation
    Metal bindingi86 – 861Zinc; catalytic1 PublicationUniRule annotation

    GO - Molecular functioni

    1. tRNA-specific adenosine-34 deaminase activity Source: UniProtKB-HAMAP
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA wobble adenosine to inosine editing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSAUR158878:GJJ5-576-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific adenosine deaminaseUniRule annotation (EC:3.5.4.33UniRule annotation)
    Gene namesi
    Name:tadAUniRule annotation
    Ordered Locus Names:SAV0558
    OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
    Taxonomic identifieri158878 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002481: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 156156tRNA-specific adenosine deaminasePRO_0000423949Add
    BLAST

    2D gel databases

    World-2DPAGE0002:Q99W51.

    Interactioni

    Subunit structurei

    Homodimer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    DIPiDIP-29048N.
    STRINGi158878.SAV0558.

    Structurei

    Secondary structure

    1
    156
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 2019
    Beta strandi27 – 326
    Beta strandi35 – 417
    Helixi44 – 474
    Helixi54 – 6613
    Beta strandi74 – 807
    Helixi84 – 929
    Beta strandi96 – 1027
    Turni105 – 1073
    Beta strandi127 – 1293
    Helixi134 – 15017

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2B3JX-ray2.00A/B/C/D1-156[»]
    ProteinModelPortaliQ99W51.
    SMRiQ99W51. Positions 1-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ99W51.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 101101CMP/dCMP deaminase zinc-bindingUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation
    Contains 1 CMP/dCMP deaminase zinc-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0590.
    HOGENOMiHOG000085050.
    KOiK11991.
    OMAiGWRLEGC.
    OrthoDBiEOG64FKGZ.
    PhylomeDBiQ99W51.

    Family and domain databases

    HAMAPiMF_00972. tRNA_aden_deaminase.
    InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    IPR028883. tRNA_aden_deaminase.
    [Graphical view]
    PfamiPF00383. dCMP_cyt_deam_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53927. SSF53927. 1 hit.
    PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99W51-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNDIYFMTL AIEEAKKAAQ LGEVPIGAII TKDDEVIARA HNLRETLQQP    50
    TAHAEHIAIE RAAKVLGSWR LEGCTLYVTL EPCVMCAGTI VMSRIPRVVY 100
    GADDPKGGCS GSLMNLLQQS NFNHRAIVDK GVLKEACSTL LTTFFKNLRA 150
    NKKSTN 156
    Length:156
    Mass (Da):17,090
    Last modified:June 1, 2001 - v1
    Checksum:iAABDDD24AF14DC12
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB56720.1.
    PIRiH89823.
    RefSeqiNP_371082.1. NC_002758.2.

    Genome annotation databases

    EnsemblBacteriaiBAB56720; BAB56720; SAV0558.
    GeneIDi1120531.
    KEGGisav:SAV0558.
    PATRICi19561768. VBIStaAur52173_0564.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB56720.1 .
    PIRi H89823.
    RefSeqi NP_371082.1. NC_002758.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2B3J X-ray 2.00 A/B/C/D 1-156 [» ]
    ProteinModelPortali Q99W51.
    SMRi Q99W51. Positions 1-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29048N.
    STRINGi 158878.SAV0558.

    2D gel databases

    World-2DPAGE 0002:Q99W51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB56720 ; BAB56720 ; SAV0558 .
    GeneIDi 1120531.
    KEGGi sav:SAV0558.
    PATRICi 19561768. VBIStaAur52173_0564.

    Phylogenomic databases

    eggNOGi COG0590.
    HOGENOMi HOG000085050.
    KOi K11991.
    OMAi GWRLEGC.
    OrthoDBi EOG64FKGZ.
    PhylomeDBi Q99W51.

    Enzyme and pathway databases

    BioCyci SAUR158878:GJJ5-576-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q99W51.

    Family and domain databases

    HAMAPi MF_00972. tRNA_aden_deaminase.
    InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR002125. CMP_dCMP_Zn-bd.
    IPR016193. Cytidine_deaminase-like.
    IPR028883. tRNA_aden_deaminase.
    [Graphical view ]
    Pfami PF00383. dCMP_cyt_deam_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53927. SSF53927. 1 hit.
    PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mu50 / ATCC 700699.
    2. "Crystal structure of Staphylococcus aureus tRNA adenosine deaminase TadA in complex with RNA."
      Losey H.C., Ruthenburg A.J., Verdine G.L.
      Nat. Struct. Mol. Biol. 13:153-159(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ZINC AND RNA, COFACTOR, SUBUNIT.
      Strain: Mu50 / ATCC 700699.

    Entry informationi

    Entry nameiTADA_STAAM
    AccessioniPrimary (citable) accession number: Q99W51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3