Q99UN8 (FABD_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malonyl CoA-acyl carrier protein transacylase
Short name=MCT
EC=2.3.1.39

Gene names

Name:	fabD
Ordered Locus Names:	SAV1230

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].
Pathway	Lipid metabolism; fatty acid biosynthesis.
Sequence similarities	Belongs to the fabD family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	[acyl-carrier-protein] S-malonyltransferase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 308

308

Malonyl CoA-acyl carrier protein transacylase

PRO_0000194224

Sites

Active site

89

1

By similarity

Active site

199

1

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

308

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Turn

20 – 25

6

Helix

27 – 39

13

Helix

44 – 49

6

Helix

59 – 76

18

Beta strand

84 – 86

3

Helix

93 – 98

6

Helix

104 – 113

10

Helix

117 – 120

4

Beta strand

130 – 133

4

Helix

137 – 147

11

Beta strand

155 – 159

5

Beta strand

165 – 168

4

Helix

171 – 179

9

Beta strand

188 – 191

4

Helix

201 – 206

6

Helix

207 – 214

8

Turn

229 – 231

3

Helix

238 – 248

11

Helix

255 – 264

10

Beta strand

269 – 272

4

Beta strand

288 – 294

7

Helix

298 – 301

4

Sequences

	Sequence		Length	Mass (Da)
	Q99UN8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B2FAEF8A200804C3	FASTA	308	33,649
	10 20 30 40 50 60 MSKTAIIFPG QGAQKVGMAQ DLFNNNDQAT EILTSAAKTL DFDILETMFT DEEGKLGETE 70 80 90 100 110 120 NTQPALLTHS SALLAALKNL NPDFTMGHSL GEYSSLVAAD VLSFEDAVKI VRKRGQLMAQ 130 140 150 160 170 180 AFPTGVGSMA AVLGLDFDKV DEICKSLSSD DKIIEPANIN CPGQIVVSGH KALIDELVEK 190 200 210 220 230 240 GKSLGAKRVM PLAVSGPFHS SLMKVIEEDF SSYINQFEWR DAKFPVVQNV NAQGETDKEV 250 260 270 280 290 300 IKSNMVKQLY SPVQFINSTE WLIDQGVDHF IEIGPGKVLS GLIKKINRDV KLTSIQTLED VKGWNEND

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000017 Genomic DNA. Translation: BAB57392.1.

RefSeq

NP_371754.1. NC_002758.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IM9	X-ray	1.46	A	5-308	[»]

ProteinModelPortal

Q99UN8.

ModBase

Search...

Protein-protein interaction databases

STRING

Q99UN8.

2D gel databases

World-2DPAGE

0002:Q99UN8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBSTAT00000006544; EBSTAP00000006362; EBSTAG00000006543.

GeneID

1121207.

GenomeReviews

Gene locus SAV1230 in contig BA000017_GR.

KEGG

sav:SAV1230.

PATRIC

19563173. VBIStaAur52173_1264.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0331.

GeneTree

EBGT00050000023910.

HOGENOM

HBG687898.

OMA

NAMQAAV.

PhylomeDB

Q99UN8.

ProtClustDB

CLSK885219.

Enzyme and pathway databases

BioCyc

SAUR158878:SAV1230-MONOMER.

Family and domain databases

InterPro

IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR024925. Malonyl_CoA-ACP_transAc.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]

Gene3D

G3DSA:3.40.366.10. Ac_transferase_reg. 2 hits.

KO

K00645.

Pfam

PF00698. Acyl_transf_1. 1 hit.
[Graphical view]

PIRSF

PIRSF000446. Mct. 1 hit.

SUPFAM

SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.

TIGRFAMs

TIGR00128. FabD. 1 hit.

ProtoNet

Search...

Entry information

Entry name

FABD_STAAM

Accession

Primary (citable) accession number: Q99UN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program