Q99UN8 (FABD_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malonyl CoA-acyl carrier protein transacylase
Short name=MCT
EC=2.3.1.39

Gene names

Name:	fabD
Ordered Locus Names:	SAV1230

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].
Pathway	Lipid metabolism; fatty acid biosynthesis.
Sequence similarities	Belongs to the FabD family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	[acyl-carrier-protein] S-malonyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 308

308

Malonyl CoA-acyl carrier protein transacylase

PRO_0000194224

Sites

Active site

89

1

By similarity

Active site

199

1

By similarity

Secondary structure

1

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308

Helix Strand Turn

Details...

Beta strand

5 – 8

4

Turn

16 – 25

10

Helix

27 – 39

13

Helix

44 – 49

6

Helix

59 – 76

18

Beta strand

83 – 88

6

Helix

91 – 98

8

Helix

104 – 120

17

Beta strand

126 – 134

9

Helix

137 – 147

11

Beta strand

154 – 161

8

Beta strand

164 – 170

7

Helix

171 – 180

10

Turn

181 – 185

5

Beta strand

187 – 191

5

Helix

201 – 206

6

Helix

207 – 214

8

Turn

229 – 231

3

Beta strand

233 – 235

3

Helix

238 – 248

11

Helix

255 – 264

10

Beta strand

267 – 276

10

Helix

278 – 286

9

Beta strand

288 – 295

8

Helix

298 – 304

7

Sequences

	Sequence		Length	Mass (Da)
	Q99UN8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: B2FAEF8A200804C3	FASTA	308	33,649
	10 20 30 40 50 60 MSKTAIIFPG QGAQKVGMAQ DLFNNNDQAT EILTSAAKTL DFDILETMFT DEEGKLGETE 70 80 90 100 110 120 NTQPALLTHS SALLAALKNL NPDFTMGHSL GEYSSLVAAD VLSFEDAVKI VRKRGQLMAQ 130 140 150 160 170 180 AFPTGVGSMA AVLGLDFDKV DEICKSLSSD DKIIEPANIN CPGQIVVSGH KALIDELVEK 190 200 210 220 230 240 GKSLGAKRVM PLAVSGPFHS SLMKVIEEDF SSYINQFEWR DAKFPVVQNV NAQGETDKEV 250 260 270 280 290 300 IKSNMVKQLY SPVQFINSTE WLIDQGVDHF IEIGPGKVLS GLIKKINRDV KLTSIQTLED VKGWNEND

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000017 Genomic DNA. Translation: BAB57392.1.

RefSeq

NP_371754.1. NC_002758.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IM9	X-ray	1.46	A	5-308	[»]

ProteinModelPortal

Q99UN8.

SMR

Q99UN8. Positions 2-305.

ModBase

Search...

Protein-protein interaction databases

STRING

158878.SAV1230.

2D gel databases

World-2DPAGE

0002:Q99UN8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAB57392; BAB57392; SAV1230.

GeneID

1121207.

KEGG

sav:SAV1230.

PATRIC

19563173. VBIStaAur52173_1264.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0331.

HOGENOM

HOG000036504.

KO

K00645.

OMA

EVSGPFH.

ProtClustDB

CLSK885219.

Enzyme and pathway databases

BioCyc

SAUR158878:GJJ5-1248-MONOMER.

UniPathway

UPA00094.

Family and domain databases

Gene3D

3.40.366.10. 2 hits.

InterPro

IPR001227. Ac_transferase_dom.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR024925. Malonyl_CoA-ACP_transAc.
IPR004410. Malonyl_CoA-ACP_transAc_FabD.
IPR016036. Malonyl_transacylase_ACP-bd.
[Graphical view]

Pfam

PF00698. Acyl_transf_1. 1 hit.
[Graphical view]

PIRSF

PIRSF000446. Mct. 1 hit.

SUPFAM

SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
SSF55048. Malonyl_transacylase_ACP-bd. 1 hit.

TIGRFAMs

TIGR00128. fabD. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q99UN8.

Entry information

Entry name

FABD_STAAM

Accession

Primary (citable) accession number: Q99UN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program