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Q99UJ8

- PNP_STAAM

UniProt

Q99UJ8 - PNP_STAAM

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi490 – 4901MagnesiumUniRule annotation
Metal bindingi496 – 4961MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1292-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Synonyms:pnpA
Ordered Locus Names:SAV1274
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 698698Polyribonucleotide nucleotidyltransferasePRO_0000260057Add
BLAST

2D gel databases

World-2DPAGE0002:Q99UJ8.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1274.

Structurei

3D structure databases

ProteinModelPortaliQ99UJ8.
SMRiQ99UJ8. Positions 7-698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini557 – 61660KHUniRule annotationAdd
BLAST
Domaini626 – 69469S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.
PhylomeDBiQ99UJ8.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99UJ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQEKKVFKT EWAGRSLTIE TGQLAKQANG AVLVRYGDTV VLSTATASKE
60 70 80 90 100
PRDGDFFPLT VNYEEKMYAA GKIPGGFKKR EGRPGDDATL TARLIDRPIR
110 120 130 140 150
PLFPKGYKHD VQIMNMVLSA DPDCSPQMAA MIGSSMALSV SDIPFQGPIA
160 170 180 190 200
GVNVGYIDGK YIINPTVEEK EVSRLDLEVA GHKDAVNMVE AGASEITEQE
210 220 230 240 250
MLEAIFFGHE EIQRLVDFQQ QIVDHIQPVK QEFIPAERDE ALVERVKSLT
260 270 280 290 300
EEKGLKETVL TFDKQQRDEN LDNLKEEIVN EFIDEEDPEN ELLIKEVYAI
310 320 330 340 350
LNELVKEEVR RLIADEKIRP DGRKPDEIRP LDSEVGILPR THGSGLFTRG
360 370 380 390 400
QTQALSVLTL GALGDYQLID GLGPEEEKRF MHHYNFPNFS VGETGPVRAP
410 420 430 440 450
GRREIGHGAL GERALKYIIP DTADFPYTIR IVSEVLESNG SSSQASICGS
460 470 480 490 500
TLALMDAGVP IKAPVAGIAM GLVTREDSYT ILTDIQGMED ALGDMDFKVA
510 520 530 540 550
GTKEGITAIQ MDIKIDGLTR EIIEEALEQA RRGRLEIMNH MLQTIDQPRT
560 570 580 590 600
ELSAYAPKVV TMTIKPDKIR DVIGPGGKKI NEIIDETGVK LDIEQDGTIF
610 620 630 640 650
IGAVDQAMIN RAREIIEEIT REAEVGQTYQ ATVKRIEKYG AFVGLFPGKD
660 670 680 690
ALLHISQISK NRIEKVEDVL KIGDTIEVKI TEIDKQGRVN ASHRALEE
Length:698
Mass (Da):77,362
Last modified:June 1, 2001 - v1
Checksum:i6EABFB86EDFBFB2E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57436.1.
RefSeqiNP_371798.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57436; BAB57436; SAV1274.
GeneIDi1121251.
KEGGisav:SAV1274.
PATRICi19563261. VBIStaAur52173_1308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000017 Genomic DNA. Translation: BAB57436.1 .
RefSeqi NP_371798.1. NC_002758.2.

3D structure databases

ProteinModelPortali Q99UJ8.
SMRi Q99UJ8. Positions 7-698.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 158878.SAV1274.

2D gel databases

World-2DPAGE 0002:Q99UJ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB57436 ; BAB57436 ; SAV1274 .
GeneIDi 1121251.
KEGGi sav:SAV1274.
PATRICi 19563261. VBIStaAur52173_1308.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.
PhylomeDBi Q99UJ8.

Enzyme and pathway databases

BioCyci SAUR158878:GJJ5-1292-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiPNP_STAAM
AccessioniPrimary (citable) accession number: Q99UJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3