Reviewed, UniProtKB/Swiss-Prot Q99U74 (ODO1_STAAN)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	odhA
Ordered Locus Names:	SA1245

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2).
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 910	910	2-oxoglutarate dehydrogenase E1 component		PRO_0000162178

Sequences

	Sequence		Length	Mass (Da)
	Q99U74-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7803AAED537CF247	FASTA	910	103,112
	10 20 30 40 50 60 MTNERKEVSE APVNFGANLG LMLDLYDDFL QDPSSVPEDL QVLFSTIKRV MRLIDNIRQY 70 80 90 100 110 120 GHLKADIYPV NPPKRKHVPK LEIEDFDLDQ QTLEGISAGI VSDHFADIYD NAYEAILRME 130 140 150 160 170 180 KRYKGPIAFE YTHINNNTER GWLKRRIETP YKVTLNNNEK RALFKQLAYV EGFEKYLHKN 190 200 210 220 230 240 FVGAKRFSIE GVDALVPMLQ RTITIAAKEG IKNIQIGMAH RGRLNVLTHV LEKPYEMMIS 250 260 270 280 290 300 EFMHTDPMKF LPEDGSLQLT AGWTGDVKYH LGGIKTTDSY GTMQRIALAN NPSHLEIVAP 310 320 330 340 350 360 VVEGRTRAAQ DDTQRAGAPT TDHHKAMPII IHGDAAYPGQ GINFETMNLG NLKGYSTGGS 370 380 390 400 410 420 LHIITNNRIG FTTEPIDARS TTYSTDVAKG YDVPIFHVNA DDVEATIEAI DIAMEFRKEF 430 440 450 460 470 480 HKDVVIDLVG YRRFGHNEMD EPSITNPVPY QNIRKHDSVE YVFGKKLVNE GVISEDEMHS 490 500 510 520 530 540 FIEQVQKELR QAHDKINKAD KMDNPDMEKP AELALPLQAD EQSFTFDHLK EINDALLTYP 550 560 570 580 590 600 DGFNILKKLN KVLEKRHEPF NKEDGLVDWA QAEQLAFATI LQDGTPIRLT GQDSERGTFS 610 620 630 640 650 660 HRHAVLHDEQ TGETYTPLHH VPDQKATFDI HNSPLSEAAV VGFEYGYNVE NKKSFNIWEA 670 680 690 700 710 720 QYGDFANMSQ MIFDNFLFSS RSKWGERSGL TLFLPHAYEG QGPEHSSARL ERFLQLAAEN 730 740 750 760 770 780 NCTVVNLSSS SNYFHLLRAQ AASLDSEQMR PLVVMSPKSL LRNKTVAKPI DEFTSGGFEP 790 800 810 820 830 840 ILTESYQADK VTKVILATGK MFIDLKEALA KNPDESVLLV AIERLYPFPE EEIEALLAQL 850 860 870 880 890 900 PKLEEVSWVQ EEPKNQGAWL YVYPYVKVLV ADKYDLSYHG RIQRAAPAEG DGEIHKLVQN 910 KIIENALKNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuye P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000018 Genomic DNA. Translation: BAB42505.1.
PIR	E89918.
RefSeq	NP_374526.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1124084.
GenomeReviews	Gene locus SA1245 in contig BA000018_GR.
KEGG	sau:SA1245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q99U74.
Enzyme and pathway databases
BioCyc	SAUR158879:SA1245-MON.
Family and domain databases
HAMAP	MF_01169. [Tree]
InterPro	IPR011603. 2oxoglutarate_DHase_E1. IPR001017. DHase_E1. IPR005475. Transketo_Cen_R. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

ODO1_STAAN

Accession

Primary (citable) accession number: Q99U74

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	June 1, 2001
Last modified:	November 4, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)