Q99TT5 (RPOD_STAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase sigma factor RpoD

Gene names

Name:	rpoD
Synonyms:	plaC, sigA
Ordered Locus Names:	SA1390

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released By similarity.
Sequence similarities	Belongs to the sigma-70 factor family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	RNA polymerase sigma factor RpoD		PRO_0000093914
Regions
	DNA binding	329 – 348	20	H-T-H motif By similarity
	Motif	159 – 172	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q99TT5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 5B1D9CACE9CFE138	FASTA	368	42,157
	10 20 30 40 50 60 MSDNTVKIKK QTIDPTLTLE DVKKQLIEKG KKEGHLSHEE IAEKLQNFDI DSDQMDDFFD 70 80 90 100 110 120 QLNDNDISLV NEKDSSDTDE KLNPSDLSAP PGVKINDPVR MYLKEIGRVN LLSAQEEIEL 130 140 150 160 170 180 AKRIEQGDEV AKSRLAEANL RLVVSIAKRY VGRGMLFLDL IQEGNMGLIK AVEKFDFNKG 190 200 210 220 230 240 FKFSTYATWW IRQAITRAIA DQARTIRIPV HMVETINKLI RVQRQLLQDL GRDPAPEEIG 250 260 270 280 290 300 EEMDLPAEKV REVLKIAQEP VSLETPIGEE DDSHLGDFIE DQEAQSPSDH AAYELLKEQL 310 320 330 340 350 360 EDVLDTLTDR EENVLRLRFG LDDGRTRTLE EVGKVFGVTR ERIRQIEAKA LRKLRHPSRS KRLKDFMD

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: N315.

[2]

"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: N315.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB42653.1.
PIR	H89936.
RefSeq	NP_374674.1. NC_002745.2.
3D structure databases
ProteinModelPortal	Q99TT5.
SMR	Q99TT5. Positions 97-368.
ModBase	Search...
Protein-protein interaction databases
STRING	158879.SA1390.
Proteomic databases
PRIDE	Q99TT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB42653; BAB42653; BAB42653.
GeneID	1124231.
KEGG	sau:SA1390.
PATRIC	19575054. VBIStaAur116463_1496.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0568.
HOGENOM	HOG000270273.
KO	K03086.
OMA	AERMSSF.
ProtClustDB	PRK09210.
Enzyme and pathway databases
BioCyc	SAUR158879:GJCB-1460-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 2 hits.
InterPro	IPR014284. RNA_pol_sigma-70_dom. IPR000943. RNA_pol_sigma70. IPR009042. RNA_pol_sigma70_r1_2. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR007127. RNA_pol_sigma_70_r1_1. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012760. RNA_pol_sigma_RpoD_C. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF03979. Sigma70_r1_1. 1 hit. PF00140. Sigma70_r1_2. 1 hit. PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
SUPFAM	SSF88946. Sigma_r2. 1 hit. SSF88659. Sigma_r3_r4. 2 hits.
TIGRFAMs	TIGR02393. RpoD_Cterm. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOD_STAAN

Accession

Primary (citable) accession number: Q99TT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program