Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Gene

mtnN

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively.UniRule annotation

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine.UniRule annotation
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11Proton acceptorUniRule annotation1
Binding sitei77Substrate; via amide nitrogenUniRule annotation1
Binding sitei151Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei196Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BRENDAi3.2.2.16 3352
3.2.2.9 3352
UniPathwayiUPA00904; UER00871

Names & Taxonomyi

Protein namesi
Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseUniRule annotation (EC:3.2.2.9UniRule annotation)
Short name:
MTA/SAH nucleosidaseUniRule annotation
Short name:
MTANUniRule annotation
Alternative name(s):
5'-methylthioadenosine nucleosidaseUniRule annotation
Short name:
MTA nucleosidaseUniRule annotation
S-adenosylhomocysteine nucleosidaseUniRule annotation
Short name:
AdoHcy nucleosidaseUniRule annotation
Short name:
SAH nucleosidaseUniRule annotation
Short name:
SRH nucleosidaseUniRule annotation
Gene namesi
Name:mtnNUniRule annotation
Ordered Locus Names:SAV1599
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003593691 – 2285'-methylthioadenosine/S-adenosylhomocysteine nucleosidaseAdd BLAST228

Proteomic databases

PaxDbiQ99TQ0

2D gel databases

World-2DPAGE0002:Q99TQ0

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1599

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 8Combined sources8
Helixi9 – 16Combined sources8
Beta strandi20 – 27Combined sources8
Beta strandi30 – 37Combined sources8
Beta strandi40 – 46Combined sources7
Helixi51 – 65Combined sources15
Beta strandi68 – 72Combined sources5
Beta strandi74 – 78Combined sources5
Beta strandi88 – 98Combined sources11
Helixi102 – 104Combined sources3
Beta strandi116 – 119Combined sources4
Helixi122 – 134Combined sources13
Beta strandi139 – 146Combined sources8
Helixi154 – 163Combined sources10
Beta strandi167 – 173Combined sources7
Helixi174 – 184Combined sources11
Beta strandi188 – 196Combined sources9
Helixi202 – 225Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BL6X-ray1.70A1-228[»]
4GMHX-ray2.00A1-228[»]
ProteinModelPortaliQ99TQ0
SMRiQ99TQ0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99TQ0

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 173Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DUF Bacteria
COG0775 LUCA
HOGENOMiHOG000259346
KOiK01243
OMAiDQFVHSK
PhylomeDBiQ99TQ0

Family and domain databases

HAMAPiMF_01684 Salvage_MtnN, 1 hit
InterProiView protein in InterPro
IPR010049 MTA_SAH_Nsdase
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01704 MTA/SAH-Nsdase, 1 hit

Sequencei

Sequence statusi: Complete.

Q99TQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGIIGAMEE EVTILKNKLT QLSEISVAHV KFYTGILKDR EVVITQSGIG
60 70 80 90 100
KVNAAISTTL LINKFKPDVI INTGSAGALD ESLNVGDVLI SDDVKYHDAD
110 120 130 140 150
ATAFGYEYGQ IPQMPVAFQS SKPLIEKVSQ VVQQQQLTAK VGLIVSGDSF
160 170 180 190 200
IGSVEQRQKI KKAFPNAMAV EMEATAIAQT CYQFNVPFVV VRAVSDLANG
210 220
EAEMSFEAFL EKAAVSSSQT VEALVSQL
Length:228
Mass (Da):24,534
Last modified:June 1, 2001 - v1
Checksum:iC756E9386E9B19DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA Translation: BAB57761.1
RefSeqiWP_000579275.1, NC_002758.2

Genome annotation databases

EnsemblBacteriaiBAB57761; BAB57761; SAV1599
KEGGisav:SAV1599

Similar proteinsi

Entry informationi

Entry nameiMTNN_STAAM
AccessioniPrimary (citable) accession number: Q99TQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health